The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads

Guan, Yue; Кемп, Р. Б.

doi:10.1007/0-306-46860-3_58

Cited by 4 publications

(8 citation statements)

References 12 publications

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“…The application of the Biomass Monitor (BM) to animal cell culture monitoring has only occurred in earnest in the last few years Degouys et al 1993;Beving et al 1994;Davey et al 1997a;Guan and Kemp 1997;Guan et al 1998;Zeiser et al 1999;Ducommun et al 2002a, b;Dowd et al 2003) for studying the growth of a wide variety of animal cells, whether in suspension or in an immobilized state. Cerkel et al (1993) investigated the dielectric properties of Chinese Hamster Ovary (CHO 320) cells and HeLa cells grown in suspension culture at a concentration of 0.5 À 3 · 10 6 cells/ml and scanned at frequencies between 0.2 and 10 MHz using a BM.…”

Section: Probe Developmentsmentioning

confidence: 99%

“…Macroporous carriers are a useful means of increasing the numbers of cells in a culture which can be low, particularly in batch cultures using CHO 320 cells (Guan and Kemp 1997). One of the problems in using macroporous carriers to cultivate animal cells in culture has been to assess cell viability on-line; this is because many of the cells inhabit the macroporous infrastructure of the bead.…”

Section: Probe Developmentsmentioning

confidence: 99%

“…One of the problems in using macroporous carriers to cultivate animal cells in culture has been to assess cell viability on-line; this is because many of the cells inhabit the macroporous infrastructure of the bead. Guan and Kemp (1997) measured the cell concentration of CHO 320 cells grown on Cytopore 1 microcarrier beads using off-line protein estimations and compared them to dielectric measurements using a BM. The results indicated that the dielectric estimations for biomasss in the microcarriers were more accurate than the protein estimations, and were also able to give a viable cell-count.…”

Section: Probe Developmentsmentioning

confidence: 99%

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On-line Measurements and Control of Viable Cell Density in Cell Culture Manufacturing Processes using Radio-frequency Impedance

Carvell¹,

2006

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In this work, radio-frequency (RF) impedance is reviewed as a method for monitoring and controlling cell culture manufacturing processes. It is clear from the many publications cited that RF Impedance is regarded as an accurate and reliable method for measuring the live cell bio-volume both on-line and off-line and the technology is also sutable for animal cells in suspension, attached to micro-carriers or immobilized in fixed beds. In cGMP production, RF Impedance is being used in three main areas. Firstly, it is being used as a control instrument for maintaining consistent perfusion culture allowing the bioreactor to operate under optimum conditions for maximum production of recombinant proteins. In the second application it has not replaced traditional off-line live cell counting techniques but it is being used as an additional monitoring tool to check product conformance. Finally, RF Impedance is being used to monitor the concentration of live cells immobilized on micro-carriers or packed beds in cGMP processes where traditional off-line live cell counting methods are inaccurate or impossible to perform.

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Section: Probe Developmentsmentioning

confidence: 99%

Section: Probe Developmentsmentioning

confidence: 99%

Section: Probe Developmentsmentioning

confidence: 99%

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On-line Measurements and Control of Viable Cell Density in Cell Culture Manufacturing Processes using Radio-frequency Impedance

Carvell¹,

2006

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“…Examples include the monitoring of HCT cells grown on Cytodex 3 in spinner flasks (Degouys et al 1993), hybridoma cells on macroporous carrier in a fluidized bed (Noll and Biselli 1998), immobilized CHO in a packed bed of FibraCell discs (Ducommun et al 2002a, b) or CHO cultures on Cytopore1 microcarrier. Results showed that with data gained from DS, more accurate information can be obtained than with data obtained by protein content determination (Guan and Kemp 2002).…”

Section: Introductionmentioning

confidence: 97%

Online- and offline- monitoring of stem cell expansion on microcarrier

et al. 2011

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In the biopharmaceutical industry, adherent growing stem cell cultures gain worldwide importance as cell products. The cultivation process of these cells, such as in stirred tank reactors or in fixed bed reactors, is highly sophisticated. Cultivations need to be monitored and controlled to guarantee product quality and to satisfy GMP requirements. With the process analytical technology (PAT) initiative, requirements regarding process monitoring and control have changed and real-time on-line monitoring tools are recommended. A tool meeting the new requirements may be the dielectric spectroscopy for online viable cell mass determination by measurement of the permittivity. To establish these tools, proper offline methods for data correlation are required. The cell number determination of adherent cells on microcarrier is difficult, as it requires cell detachment from the carrier, which highly increases the statistical error. As an offline method, a fluorescence assay based on SYBR Ò GreenI was developed allowing fast and easy total cell concentration determination without the need to detach the cells from the carrier. The assay is suitable for glass carriers used in stirred tank reactor systems or in fixed bed systems, may be suitable for different cell lines and can be applied to high sample numbers easily. The linear dependency of permittivity to cell concentration of suspended stem cells with the dielectric spectroscopy is shown for even very small cell concentrations. With this offline-method, a correlation of the cell concentration grown on carrier to the permittivity data measured by the dielectric spectroscopy was done successfully.

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“…The application of the Biomass Monitor to animal cell culture monitoring has only occurred in earnest in the last few years Degouys et al, 1993;Beving et al, 1994;Noll, 1995;Noll et al, 1996;Noll and Biselli, 1998;Davey et al, 1997;Guan and Kemp, 1997;Guan et al, 1998;Zeiser et al, 1999). In these works the Biomass Monitor has been used to study the growth of a wide variety of animal cells whether in suspension or in an immobilised state.…”

mentioning

confidence: 99%

On-Line, Real-Time Measurements of Cellular Biomass using Dielectric Spectroscopy

Yardley

Kell

Barrett

et al. 2000

Biotechnology and Genetic Engineering Reviews

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The Viable Cell Monitor: A Dielectric spectroscope for Growth and Metabolic Studies of Animal Cells on Macroporous Beads

Cited by 4 publications

References 12 publications

On-line Measurements and Control of Viable Cell Density in Cell Culture Manufacturing Processes using Radio-frequency Impedance

On-line Measurements and Control of Viable Cell Density in Cell Culture Manufacturing Processes using Radio-frequency Impedance

Online- and offline- monitoring of stem cell expansion on microcarrier

On-Line, Real-Time Measurements of Cellular Biomass using Dielectric Spectroscopy

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