Previous research has established that pitch and timbre perceptually interact within a variety of tasks, but has not fully established the causes of this interaction. Using synthesized missing fundamental stimuli, the present study investigated the possibility that 2 acoustic characteristics commonly associated with timbre, spectral centroid and spectral envelope shape, affect pitch judgments. Listeners were asked to determine the pitch relationship (ascending/descending) between 2 sequential tones: a standard tone (A 4 ) and a comparison tone (either A 4 , A # 4 , or F 5 ). The comparison tone either included, or lacked, fundamental frequency (F 0 ). For A 4 and A # 4 , alternative versions of the Missing F 0 comparison tones also were created that were low-pass filtered such that the spectral centroid matched the standard. When the fundamental of the comparison tone was higher than that of the standard A 4 tone, removing its F 0 (and thus, increasing centroid) led to increased reports of ascending pitch change. When shifts in centroid were eliminated through filtering (A 4 and A # 4 comparison tones), the impact of removing F 0 was not observed, suggesting that spectral centroid influenced pitch comparison to a greater degree than envelope shape. In contrast, when the chroma for the standard and comparison tones were identical (i.e., both were A 4 ), differences in spectral envelope shape led to increased reports of different pitches, while shifts in spectral centroid had no apparent effect. Furthermore, listeners with more years of musical training tended to be less affected by spectral manipulations in making pitch judgments. Thus, it appears that different aspects of timbre affect pitch judgments in different ways depending on whether or not the tones to be compared are based on the same fundamental frequency.