The viscoelastic properties of the cervical mucus plug

Bastholm, Sara Kjær; Becher, Naja; Stubbe, P.; Chronakis, Ioannis S.; Uldbjerg, Niels

doi:10.1111/aogs.12308

Cited by 9 publications

(15 citation statements)

References 27 publications

(37 reference statements)

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“…A G ″ overshoot during stress amplitude sweeps was rarely mentioned in the literature on the rheological characterization of mucus. In the work of Bastholm, a G ″ overshoot seems to occur with some of the tested cervical mucus samples, without any comment concerning this phenomenon. Celli et al, performing a SAOS stress amplitude sweep on a healthy gastric mucus sample (compared to a Helicobacter pylori infected one), also observed a “weak strain overshoot” on G ″ and refered to the work of Huyn et al for interpretation.…”

Section: Discussionmentioning

confidence: 92%

“…Previous works devoted to the study of mucus rheology (synthetic or native mucus from different organs), have only described part of its rheological properties. For instance, many studies have only used dynamic oscillatory shear measurements to characterize mucus rheology . Under small deformations (SAOS) these measurements mostly reflect the properties of mucus under its unperturbed state and are useful to describe the linear viscoelastic (LVE) response of mucus.…”

Section: Introductionmentioning

confidence: 99%

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Rheological properties of synthetic mucus for airway clearance

Lafforgue

Seyssiecq

Poncet

et al. 2017

J Biomedical Materials Res

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In this work, a complete rheological characterization of bronchial mucus simulants based on the composition proposed by Zahm et al. (Eur Respir J 1991; 4:311-315) is presented. Dynamic small amplitude oscillatory shear (SAOS) experiments, steady state (SS) flow measurements and three intervals thixotropy tests (3ITT), are carried out to investigate the global rheological complexities of simulants (viscoelasticity, viscoplasticity, shear-thinning, and thixotropy) as a function of scleroglucan concentrations (0.5-2 wt %) and under temperatures of 20 and 37°C. SAOS measurements show that the limit of the linear viscoelastic range as well as the elasticity both increase with increasing sclerogucan concentrations. Depending on the sollicitation frequency, the 0.5 wt % gel response is either liquid-like or solid-like, whereas more concentrated gels show a solid-like response over the whole frequency range. The temperature dependence of gels response is negligible in the 20-37°C range. The Herschel-Bulkley (HB) model is chosen to fit the SS flow curve of simulants. The evolution of HB parameters versus polymer concentration show that both shear-thinning and viscoplasticity increase with increasing concentrations. 3ITTs allow calculation of recovery thixotropic times after shearings at 100 or 1.6 s . Empiric correlations are proposed to quantify the effect of polymer concentration on rheological parameters of mucus simulants. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 386-396, 2018.

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Section: Discussionmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Rheological properties of synthetic mucus for airway clearance

Lafforgue

Seyssiecq

Poncet

et al. 2017

J Biomedical Materials Res

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“…All CMP specimens showed solid-like viscoelastic behavior with predominating elastic properties, as seen in the representative example shown in Figure 1(a,b). We found that the elastic modulus (G') was about three to four times greater than the viscous modulus (G") at all frequencies (13). Likelihood ratio tests evaluated to what extent the TFF variation between the specimens reflected differences between CMPs or differences within the CMPs, i.e.…”

Section: Resultsmentioning

confidence: 98%

“…The method for assessing rheological properties of the CMP including determining the linear viscoelastic region has previously been described (13). Briefly, the rheological properties were assessed using a dynamic oscillatory rheometer (Stress Tech, Reologica instruments AB, Lund, Sweden) within the linear viscoelastic region and at 37.0°C.…”

Section: Methodsmentioning

confidence: 99%

“…The Trefoil factor peptides (TFFs) constitute a family of small (12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22) kDa) molecules containing one (TFF1 and TFF3) or two (TFF2) trefoil domains (7). TFFs are most abundantly expressed in the gastrointestinal tract, yet they can be detected in virtually all tissues containing mucin-secreting cells including the endocervix and the uterus (7,8).…”

Section: Key Messagementioning

confidence: 99%

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Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

Bastholm

Samson

Becher

et al. 2016

Acta Obstet Gynecol Scand

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Introduction The viscoelastic properties of the cervical mucus plug are considered essential for the occlusion of the cervical canal and thereby for protection against ascending infections during pregnancy. Factors controlling this property are virtually unknown. This study explores a possible role of trefoil factor peptides 1, 2 and 3 (TFF1–3); peptides believed to influence mucus viscosity. Material and methods The study is based on spontaneously shed cervical mucus plugs from 14 women in active labor. The viscoelastic properties; the elastic modulus (G') and the viscous modulus (G”) were determined by an oscillatory rheometer. The concentrations of TFF1–3 were measured by an in‐house enzyme‐linked immunosorbent assay. Associations were analyzed by random‐effects generalized least‐squares regression analyses. Results Median (range) concentrations of TFF1, TFF2 and TFF3 were 3.1 (1.2–8.6), 1.1 (<0.006–3.7) and 1000 (170–5300) nmol/g cervical mucus plug, respectively. The TFF3 concentration was associated with G' (regression coefficient 11.7 Pa/Log nm; 95% CI 3.0–20.4, p = 0.009) and G” (regression coefficient 3.2 Pa/Log nm; 95% CI 1.5–5.0, p < 0.001). Conclusion We suggest that TFF3 plays a role in the viscoelastic properties of the cervical mucus plug.

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The yielding behaviour of human mucus

Kavishvar,

Ramachandran

2023

Advances in Colloid and Interface Science

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The viscoelastic properties of the cervical mucus plug

Cited by 9 publications

References 27 publications

Rheological properties of synthetic mucus for airway clearance

Rheological properties of synthetic mucus for airway clearance

Trefoil factor peptide 3 is positively correlated with the viscoelastic properties of the cervical mucus plug

The yielding behaviour of human mucus

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