The visible to the near infrared narrow band acousto-optic tunable filter and the hyperspectral microscopic imaging on biomedicine study

Abstract

Based on the parallel tangents momentum-matching condition, a narrow band noncollinear acousto-optic tunable filter (AOTF) using a single TeO2 crystal is designed with the consideration of the birefringence and the rotatory property of the material. An effective setup is established to evaluate the performance of the designed AOTF. The experimental observed spectrum pattern of the diffracted light is nearly the same with the theoretical result. The measured tuning relationship between the diffracted central optical wavelength and acoustic frequency is in accordance with the theoretical prospect. The optical bandwidth of the diffracted light is as narrow as 1.88 nm when the central wavelength is 556.75 nm. The high spectral resolution is significant in practical applications of imaging AOTF. Additionally, the AOTF based hyperspectral microscopic imaging system is established. The stability and the image resolution of the designed narrow band AOTF are satisfying. Finally, the study of the biologic samples indicates the feasibility of our system on biomedicine.

Simultaneous imaging of temperature and concentration of ethanol–water mixtures in microchannel using near-infrared dual-wavelength absorption technique

“…The frame rates of filter exchange systems are, in principle, higher than those of linescan hyperspectral imaging systems [25,28,29]. Acoustooptic tunable filter (AOTF) is a solid-state device for spectral imaging [60][61][62] but its active aperture is too small for staring imaging. Of liquid crystal tunable filter (LCTF) [63][64][65][66], the frame rate is lower than that of filter exchange systems, and its transmission bandwidth is larger than that of the NBPF, especially for an NIR region.…”

The Calibration Methods of Geometric Parameters of Crystal for Mid-Infrared Acousto-Optic Tunable Filter-Based Imaging Systems Design

“…The acousto-optic tunable filter (AOTF) has been widely applied in spectral imaging systems for remote sensing [ 1 , 2 ], spectral microscopy [ 3 , 4 ], three-dimensional detection [ 5 ], notch filtering [ 6 , 7 ], and edge enhancement [ 8 ] due to its advantages of a narrow bandpass, electric tunability, all-solid-state, fast response, large angular aperture, and wide spectral range. In particular, the flexible electric tunability makes AOTF spectral imagers the most promising candidates for identifying and tracking targets.…”

Focus prediction of medical microscopic images based on Lightweight Densely Connected with Squeeze-and-Excitation Network

“…Nowadays, microscopy is still the most frequently used microscopic detection technology for examining thin sections and stained tissue sections on slides, playing an irreplaceable role in biomedicine, materials chemistry, industrial inspection, and other aspects ( Ikeda et al, 2009 ; Zhang et al, 2014 ; Carrera et al, 2017 ). When the microscope is used for imaging living cells, defocus blur may occur due to thermal fluctuation of the microscope body and the movement of the microscope sample ( Kreft et al, 2005 ).…”