The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator

McCarty, Donald R.; Hattori, Tsukaho; Carson, Christian B.; Vasil, Vimla; Lazar, M. D.; Vasil, Indra K.

doi:10.1016/0092-8674(91)90436-3

Cited by 622 publications

(536 citation statements)

References 47 publications

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“…The yeast GAL4 acidic activator, for instance, can activate transcription in tobacco (35). It has also been shown that certain plant transcription factors, such as VP1 (36), have acidic domains that function as transcriptional activators in plants. Significantly, the acidic activation domains of the VP16 and GCN4 transcription factors require the ''adaptor'' proteins ADA2, ADA3, and GCN5 for full activity in yeast (37).…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Stockinger

Gilmour

Thomashow

1997

Proc. Natl. Acad. Sci. U.S.A.

1,588

1,203

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Recent efforts have defined a cis-acting DNA regulatory element in plants, the C-repeat͞dehydration responsive element (DRE), that stimulates transcription in response to low temperature and water deficit. Here we report the isolation of an Arabidopsis thaliana cDNA that encodes a C-repeat͞DRE binding factor, CBF1 (C-repeat͞DRE Binding Factor 1). Analysis of the deduced CBF1 amino acid sequence indicates that the protein has a molecular mass of 24 kDa, a potential nuclear localization sequence, and a possible acidic activation domain. CBF1 also has an AP2 domain, which is a DNA-binding motif of about 60 aa present in the Arabidopsis proteins APETALA2, AINTEGUMENTA, and TINY; the tobacco ethylene response element binding proteins; and numerous other plant proteins of unknown function. The transcript levels for CBF1, which appears to be a single or low copy number gene, did not change appreciably in plants exposed to low temperature or in detached leaves subjected to water deficit. Binding of CBF1 to the C-repeat͞DRE was demonstrated in gel shift assays using recombinant CBF1 protein expressed in Escherichia coli. Moreover, expression of CBF1 in yeast was found to activate transcription of reporter genes containing the C-repeat͞DRE as an upstream activator sequence but not mutant versions of the DNA element. We conclude that CBF1 can function as a transcriptional activator that binds to the C-repeat͞DRE DNA regulatory element and, thus, is likely to have a role in cold-and dehydrationregulated gene expression in Arabidopsis.

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Section: Discussionmentioning

confidence: 99%

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Stockinger

Gilmour

Thomashow

1997

Proc. Natl. Acad. Sci. U.S.A.

1,588

1,203

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“…Lesions in the genes encoding these factors underlie some of the phenotypic characteristics of mutants that are disrupted in seed maturation. For example, the VP1 gene of cereals and the ABI3 gene of Arabidopsis encode transcription factors that regulate the expression of ABA-responsive genes during seed development (for example, storage-protein genes and LEA genes) (McCarty and others 1991;Giraudat and others 1992). The encoded proteins belong to the B3 domain family of transcription factors; all ABI3/ VP1 proteins contain four conserved domains: an acidic activation domain and three basic domains, B1, B2, and B3.…”

Section: Aba Signaling Pathway Components: Putative Roles In Developmmentioning

confidence: 99%

Role of Abscisic Acid in Seed Dormancy

Kermode

2005

J Plant Growth Regul

251

160

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Seed dormancy is an adaptive trait that improves survival of the next generation by optimizing the distribution of germination over time. The agricultural and forest industries rely on seeds that exhibit high rates of germination and vigorous, synchronous growth after germination; hence dormancy is sometimes considered an undesirable trait. The forest industry encounters problems with the pronounced dormancy of some conifer seeds, a feature that can lead to non-uniform germination and poor seedling vigor. In cereal crops, an optimum balance is most sought after; some dormancy at harvest is favored because it prevents germination of the physiologically mature grain in the head prior to harvest (that is, preharvest sprouting), a phenomenon that leads to considerable damage to grain quality and is especially prominent in cool moist environments. The sesquiterpene abscisic acid (ABA) regulates key events during seed formation, such as the deposition of storage reserves, prevention of precocious germination, acquisition of desiccation tolerance, and induction of primary dormancy. Its regulatory role is achieved in part by cross-talk with other hormones and their associated signaling networks, via mechanisms that are largely unknown. Quantitative genetics and functional genomics approaches will contribute to the elucidation of genes and proteins that control seed dormancy and germination, including components of the ABA signal transduction pathway. Dynamic changes in ABA biosynthesis and catabolism elicit hormone-signaling changes that affect downstream gene expression and thereby regulate critical checkpoints at the transitions from dormancy to germination and from germination to growth. Some of the recent developments in these areas are discussed.

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“…Two of these genes have been cloned (Giraudat et al, 1992;Cutler et al, 1996). The A613 gene (Giraudat et al, 1992), by homology to the maize WVIPAROUSI gene (McCarty et al, 1991), encodes a putative transcription factor essential for mediating a subset of the seed developmental programs (Ooms et al, 1993;Parcy et al, 1994;Nambara et al, 1995). This is corroborated by ectopic expression of the wild-type AB13, which rendered vegetative tissues hypersensitive to 'ABA and activated expression of several otherwise seed-specific genes in leaves when exogenous ABA was supplied (Parcy et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

1997

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Abscisic acid (ABA) mediates seed maturation and adaptive responses t o environmental stress. In Arabidopsis, the ABA-INSENSITIVEl (ABll) protein phosphatase 2C is required for proper ABA responsiveness both in seeds and in vegetative tissues. To determine whether the lack of recessive alleles at the corresponding locus could be explained by the existence of redundant genes, we initiated a search for ABl7 homologs. One such homolog turned out t o be the A612 locus, whose abi2-7 mutation was previously known t o decrease ABA sensitivity. Whereas abi7-7 is (semi)dominant, abi2-7 has been described as recessive and maternally controlled at the germination stage. Unexpectedly, the sequence of the abi2-7 mutation showed that it converts Gly-168 t o Asp, which is precisely the same amino acid substitution found in abi7-7 and at the coincidental position within the A B l l phosphatase domain (Gly-180 t o Asp). In vitro assays and functional complementation studies in yeast confirmed that the AB12 protein is an active protein phosphatase 2C and that the abi2-7 mutation reduced phosphatase activity as well as affinity t o Mg2+. Although a number of differences between the two mutants in adaptive responses t o stress have been reported, quantitative comparisons of other major phenotypes showed that the effects of both abi7-7 and abi2-7 on these processes are nearly indistinguishable. Thus, the homologous ABll and AB12 phosphatases appear t o assume partially redundant functions in ABA signaling, which may provide a mechanism t o maintain informational homeostasis.

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The Viviparous-1 developmental gene of maize encodes a novel transcriptional activator

Cited by 622 publications

References 47 publications

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Arabidopsis thaliana CBF1 encodes an AP2 domain-containing transcriptional activator that binds to the C-repeat/DRE, a cis-acting DNA regulatory element that stimulates transcription in response to low temperature and water deficit

Role of Abscisic Acid in Seed Dormancy

The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction.

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