The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte

Maiwulanjiang, Maitinuer; Chen, Jianping; Xin, Gui-Zhong; Gong, Amy G. W.; Miernisha, Abudureyimu; Du, Yingqing; Lau, Kei-man; Lee, Pinky S.C.; Chen, Jihang; Dong, Tina Tingxia; Aisa, Haji Akber; Tsim, Karl Wah Keung

doi:10.1016/j.jep.2014.03.010

Cited by 28 publications

(19 citation statements)

References 44 publications

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“…The formation of ROS was revealed by laser confocal employing DCF fluorescent dye [19]. The formation of ROS in cultures was in a dose-dependent manner in application of tBHP.…”

Section: Resultsmentioning

confidence: 99%

“…The formation of ROS was evaluated using the oxidation-sensitive dye 2’, 7’-dichlorofluorescin diacetate (DCFH-DA) [19]. Cultured cells were pre-treated with tBHQ or herbal extract for 24 hours, then cells were labeled with 10 μM DCFH-DA in HEPES for 1 hour at room temperature.…”

Section: Methodsmentioning

confidence: 99%

“…After washing three times with HEPES, the cells were treated with tBHP for 1 hour at room temperature. The amount of intracellular tBHP-induced ROS formation was detected by fluorometric measurement with excitation at 485 nm and emission at 530 nm [19]. …”

Section: Methodsmentioning

confidence: 99%

“…Followed by centrifugation at 13,200 rpm 10 min, the supernatant was collected and used to perform luciferase assay (Tropix Inc., Bedford, MA). The transfection efficiency in H9C2 cells was 40%, as determined by another control plasmid of having a β-galactosidase, under a cytomegalovirus enhancer promoter [19]. …”

Section: Methodsmentioning

confidence: 99%

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Ferulic Acid Orchestrates Anti-Oxidative Properties of Danggui Buxue Tang, an Ancient Herbal Decoction: Elucidation by Chemical Knock-Out Approach

et al. 2016

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Ferulic acid, a phenolic acid derived mainly from a Chinese herb Angelica Sinensis Radix (ASR), was reported to reduce the formation of free radicals. Danggui Buxue Tang (DBT), a herbal decoction composing of Astragali Radix (AR) and ASR, has been utilized for more than 800 years in China having known anti-oxidative property. Ferulic acid is a major active ingredient in DBT; however, the role of ferulic acid within the herbal mixture has not been resolved. In order to elucidate the function of ferulic acid within this herbal decoction, a ferulic acid-depleted herbal decoction was created and named as DBTΔfa. The anti-oxidative properties of chemically modified DBT decoction were systemically compared in cultured H9C2 rat cardiomyoblast cell line. The application of DBT and DBTΔfa into the cultures showed functions in (i) decreasing the reactive oxygen species (ROS) formation, detected by laser confocal; (ii) increasing of the activation of Akt; (iii) increasing the transcriptional activity of anti-oxidant response element (ARE); and (iv) increasing the expressions of anti-oxidant enzymes, i.e. NQO1 and GCLM. In all scenario, the aforementioned anti-oxidative properties of DBTΔfa in H9C2 cells were significantly reduced, as compared to authentic DBT. Thus, ferulic acid could be an indispensable chemical in DBT to orchestrate multi-components of DBT as to achieve maximal anti-oxidative functions.

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“…The formation of ROS was revealed by laser confocal employing DCF fluorescent dye [19]. The formation of ROS in cultures was in a dose-dependent manner in application of tBHP.…”

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Ferulic Acid Orchestrates Anti-Oxidative Properties of Danggui Buxue Tang, an Ancient Herbal Decoction: Elucidation by Chemical Knock-Out Approach

et al. 2016

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“…[8] As oxygen-based chemical intermediates with high reactivity, the increase production of ROS was a causal feature in the toxicity of many xenobiotics. [910] Excessive intake of alcohol could enhance the production of ROS and restrain the activity of antioxidants in vivo , such as superoxide dismutase (SOD), glutathione (GSH), and NAD(P)H: quinone oxidoreductase.…”

Section: Introductionmentioning

confidence: 99%

Soyasaponin bb protects rat hepatocytes from alcohol-induced oxidative stress by inducing heme oxygenase-1

Zhu¹,

Ranran²,

Liu³

et al. 2016

Phcog Mag

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Background:It has been known that oxidative stress induced by alcohol played a crucial role in the formation of alcoholic liver disease. Although the formation mechanisms underlying liver injury induced by alcohol still remained largely unknown, it has been considered that oxidative stress played a core role in the pathogenesis of hepatocyte damage.Objective:The aim of this study was to investigate the effects of soyasaponin Bb (Ss-Bb) on oxidative stress in alcohol-induced rat hepatocyte injury.Results:It has been shown that the administration of Ss-Bb could significantly restore antioxidant activity in BRL 3A cells. Moreover, the impaired liver function and morphology changes resulting from ethanol exposure were improved by Ss-Bb treatment. Treatment with a pharmacological inhibitor of haem oxygenase-1 (HO-1) indicated a critical role of HO-1 in mediating the protective role. Finally, we found that pretreatment with Ss-Bb to ethanol exposure cells increased the expression level of HO-1.Conclusion:It was suggested that Ss-Bb may protect against alcohol-induced hepatocyte injury through ameliorating oxidative stress, and the induction of HO-1 was an important protective mechanism.SUMMARY Effects of soyasaponin Bb was investigated on oxidative stress in rat hepatocytesCell viability and antioxidant capacities were evaluated to determine the effectsThe expression level of HO-1 was measured to reveal the proptective mechanisms

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Screening and identification of effective components from modified Taohong Siwu decoction for protecting H9c2 cells from damage

Wang

et al. 2023

In Vitro Cell.Dev.Biol.-Animal

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The volatile oil of Nardostachyos Radix et Rhizoma inhibits the oxidative stress-induced cell injury via reactive oxygen species scavenging and Akt activation in H9c2 cardiomyocyte

Cited by 28 publications

References 44 publications

Ferulic Acid Orchestrates Anti-Oxidative Properties of Danggui Buxue Tang, an Ancient Herbal Decoction: Elucidation by Chemical Knock-Out Approach

Ferulic Acid Orchestrates Anti-Oxidative Properties of Danggui Buxue Tang, an Ancient Herbal Decoction: Elucidation by Chemical Knock-Out Approach

Soyasaponin bb protects rat hepatocytes from alcohol-induced oxidative stress by inducing heme oxygenase-1

Screening and identification of effective components from modified Taohong Siwu decoction for protecting H9c2 cells from damage

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