The voltammetric determination of peroxynitrite at a mercury film electrode

Захарова, Э. А.; Yurmazova, T. A.; Nazarov, B. F.; Wildgoose, Gregory G.; Compton, Richard G.

doi:10.1039/b615188d

Cited by 9 publications

(4 citation statements)

References 21 publications

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“…Peroxynitrite is a strong oxidant [E′ o 1.4 v for ONOO − /NO − 3 (Zakharova et al, 2007)]. However, the many of its biological effects can be due to the reactions of its decomposition product, NO 2 (Pacher et al, 2007) [E′ o 0.8 v for NO 2 /NO − 3 (Standard electrode potential data)].…”

Section: Resultsmentioning

confidence: 99%

Bacillus anthracis co-opts nitric oxide and host serum albumin for pathogenicity in hypoxic conditions

John¹,

Blower²,

Popova³

et al. 2013

Front. Cell. Infect. Microbiol.

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Bacillus anthracis is a dangerous pathogen of humans and many animal species. Its virulence has been mainly attributed to the production of Lethal and Edema toxins as well as the antiphagocytic capsule. Recent data indicate that the nitric oxide (NO) synthase (baNOS) plays an important pathogenic role at the early stage of disease by protecting bacteria from the host reactive species and S-nytrosylating the mitochondrial proteins in macrophages. In this study we for the first time present evidence that bacteria-derived NO participates in the generation of highly reactive oxidizing species which could be abolished by the NOS inhibitor L - NAME, free thiols, and superoxide dismutase but not catalase. The formation of toxicants is likely a result of the simultaneous formation of NO and superoxide leading to a labile peroxynitrite and its stable decomposition product, nitrogen dioxide. The toxicity of bacteria could be potentiated in the presence of bovine serum albumin. This effect is consistent with the property of serum albumin to serves as a trap of a volatile NO accelerating its reactions. Our data suggest that during infection in the hypoxic environment of pre-mortal host the accumulated NO is expected to have a broad toxic impact on host cell functions.

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Section: Resultsmentioning

confidence: 99%

Bacillus anthracis co-opts nitric oxide and host serum albumin for pathogenicity in hypoxic conditions

John¹,

Blower²,

Popova³

et al. 2013

Front. Cell. Infect. Microbiol.

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show abstract

“…Hb is used as the mimetic enzyme for substituting horseradish peroxidase for two reasons: (1) for the purpose of the mildness of reaction conditions and the minimization of cost and (2) Hb is a kind of nature protein in human body and it is much likely to coexist with peroxynitrite in cell or organism (Liang et al 2005). Other peroxynitrite determination methods are direct voltammetric detection at a mercury film electrode at alkaline pH (Zakharova et al 2007) and luminol-enhanced chemiluminescence methods (Magalhaes et al 2008).…”

Section: Measurement Of Other Reactive Oxygen/nitrogen Speciesmentioning

confidence: 99%

Review of Methods to Determine Antioxidant Capacities

2009

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Antioxidant capacity is related with compounds capable of protecting a biological system against the potentially harmful effect of processes or reactions involving reactive oxygen and nitrogen species (ROS and RNS). These protective effects of antioxidants have received increasing attention within biological, medical, nutritional, and agrochemical fields and resulted in the requirement of simple, convenient, and reliable antioxidant capacity determination methods. Many methods which differ from each other in terms of reaction mechanisms, oxidant and target/probe species, reaction conditions, and expression of results have been developed and tested in the literature. In this review, the methods most widely used for the determination of antioxidant capacity are evaluated, presenting the general principals, recent applications, and their strengths and limitations. Analysis conditions, substrate, and antioxidant concentration should simulate real food or biological systems as much as possible when selecting the antioxidant capacity method. The total antioxidant capacity value should include methods applicable to both lipophilic and hydrophilic antioxidants, with regards the similarity and differences of both hydrogen atom transfer and electron transfer mechanism. The methods including various ROS/RNS also have to be designed to comprehensively evaluate the antioxidant capacity of a sample.

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“…62 While sufficiently stable for 30 to 60 minutes in alkaline solutions (pH z 10), 17 peroxynitrite decomposes in less than 1 s at neutral pH. 65 Since using alkaline buffer PON solutions is customary in electrochemical sensing methods, 66,67 the following experiments were conducted in CAPS buffer (pH 10). Fig.…”

Section: Electrocatalytic Response Rgo/copc-cooh Modied Glassy Carbo...mentioning

confidence: 99%

Cobalt phthalocyanine tetracarboxylic acid modified reduced graphene oxide: a sensitive matrix for the electrocatalytic detection of peroxynitrite and hydrogen peroxide

et al. 2015

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The voltammetric determination of peroxynitrite at a mercury film electrode

Cited by 9 publications

References 21 publications

Bacillus anthracis co-opts nitric oxide and host serum albumin for pathogenicity in hypoxic conditions

Bacillus anthracis co-opts nitric oxide and host serum albumin for pathogenicity in hypoxic conditions

Review of Methods to Determine Antioxidant Capacities

Cobalt phthalocyanine tetracarboxylic acid modified reduced graphene oxide: a sensitive matrix for the electrocatalytic detection of peroxynitrite and hydrogen peroxide

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