The volume change resulting from stimulation of a giant nerve fibre

Hill, D. K.

doi:10.1113/jphysiol.1950.sp004481

Cited by 133 publications

(64 citation statements)

References 8 publications

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“…This value is in agreement with that estimated by Hill (1950) from the measurements of the volume change of an axon after repetitively elicited action potentials, but it is one order of magnitude larger than that given by Caldwell and Keynes (1960) from the 36C1-influx measurements.…”

Section: The Chloride Permeabilitysupporting

confidence: 80%

Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives.

Inoue¹

1985

The Journal of General Physiology

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When giant axons of squid, Sepioteuthis, were bathed in a 100 mM Ca-salt solution containing tetrodotoxin (TTX) and internally perfused with a solution of 100 mM tetraethylammonium-salt (TEA-salt) or tetramethylammonium-salt (TMA-salt), the membrane potential was found to become sensitive to anions, especially Cl-. Membrane currents recorded from those axons showed practically no time-dependent properties, but they had a strong voltage-dependent characteristic, i.e., outward rectification. Cl- had a strong effect upon the voltage-dependent membrane currents. The nonlinear property of the currents was almost completely suppressed by some disulfonic stilbene derivatives applied intracellularly, such as 4-acetoamido-4'-isothiocyanostilbene-2,2'-disulfonic acid (SITS) and as 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid (DIDS), which are blockers of chloride transport. On the basis of these experimental results, it is concluded that a voltage-dependent chloride-permeable channel exists in the squid axon membrane. The chloride permeability (PCl) is a function of voltage, and its value at the resting membrane (Em = -60 mV) is calculated, using the Goldman-Hodgkin-Katz equation, to be 3.0 X 10(-7) cm/s.

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Section: The Chloride Permeabilitysupporting

confidence: 80%

Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives.

Inoue¹

1985

The Journal of General Physiology

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“…This possibility is discussed by Jack (1975Jack ( , 1976. Hill (1950) showed that in the giant axon of the squid following a period of activity there is an increase in volume due to a net, inward movement of salt and water. In the squid axon the over-all effect is small, A typical group III or small y axon has a perimeter of about 8 ,tm (Arbuthnott, Boyd & Kalu, 1980, Fig.…”

Section: Swelling Of Axons In Vivomentioning

confidence: 99%

Quantitative study of the non‐circularity of myelinated peripheral nerve fibres in the cat

Arbuthnott

Ballard

Boyd

et al. 1980

The Journal of Physiology

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SUMMARY1. One hind limb of each of four cats was either chronically de-efferentated, or chronically de-afferentated, and perfused with buffered glutaraldehyde fixative. Up to three different muscle nerves were dissected from each limb, post-fixed in osmium tetroxide and embedded in Epon. Ultrathin transverse sections were mounted on Formvar-coated single-hole specimen grids so that all the fibres in each nerve could be examined individually by electron microscopy.2. Non-circularity was expressed as the ratio (0): axon cross-sectional area area of a circle with same perimeter'The degree of non-circularity of all the afferent axons, or all the efferent axons, in each muscle nerve was determined. The proportion of fibres cut through the paranodal region, or through the Schwann cell nucleus, was as expected for group I afferent and for a and y efferent fibres, but hardly any typical paranodal sections of group II or III afferent fibres were encountered which suggests that their paranodal arrangement differs from that of other groups. In a quantitative comparison of noncircularity in different functional groups, fibres cut through paranodes, Schwann cell nuclei or Schmidt-Lanterman clefts were rejected.3. All the y efferent fibres in one nerve were studied in a series of sections cut at 25 ,tm intervals. The degree of non-circularity was found to be relatively constant along the internode of most fibres when the values at paranodes, Schwann cell nuclei or Schmidt-Lanterman clefts were ignored.4. The value of 0 varied widely from 1-0 (circular) to 0-5 or less from fibre to fibre within every functional group. However, the mean value of qS was less for y axons (0.68) than for a axons (0.78), and less for group III axons (0.79) than for axons in groups I and II (both 0.84). When the results for all the nerves were aggregated, these differences were statistically very highly significant, as was the difference in qS between group I and a fibres. If values of qS < 0 5 were rejected, the difference between the between a and y fibres was still very highly significant. 5. The external perimeter (S) of a non-circular fibre differs from 2i times the diameter of a circle just enclosing the fibre (D). It is shown that S = 0 95 7TD for group I and II fibres, S = 0 90 nrD for a and group III fibres, and S = 0-85 iD for y fibres.6. The myelin period, or interperiod repeat distance, varied from 14 1 to 15-6 nm in different cats, implying radial shrinkage of the myelin sheath from 15 to 23% The myelin period in a particular cat was the same for several nerves, and the same for fibres in different functional groups.7. The possibility that repetitive firing of axons during fixation contributed to the varying degree of non-circularity is considered but rejected as unlikely.8. It is deduced that about 10 % radial shrinkage of the myelin sheath, but little or no osmotic shrinkage of the axon, occurred during fixation and rinsing. Further radial shrinkage of about 8 % in all components of the fibre probably occurred as a result of subsequen...

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“…to bring the K+ ion concentration down to 150 mm. An approximate value for this rate of osmotic change may be derived from measurements on the giant axons of cephalopods (Hill, 1950). Making due allowance for the different volume to surface ratios, the time constant for osmotic equilibration would lie between 4 and 8 sec for the motoneurone.…”

Section: (B) Ionic Injection By Hyperpolarizing Currentsmentioning

confidence: 99%

The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post‐synaptic potential

Coombs¹,

Eccles²,

Fatt³

1955

The Journal of Physiology

543

223

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The volume change resulting from stimulation of a giant nerve fibre

Cited by 133 publications

References 8 publications

Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives.

Voltage-dependent chloride conductance of the squid axon membrane and its blockade by some disulfonic stilbene derivatives.

Quantitative study of the non‐circularity of myelinated peripheral nerve fibres in the cat

The specific ionic conductances and the ionic movements across the motoneuronal membrane that produce the inhibitory post‐synaptic potential

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