The water-soluble B-vitamins

Water-soluble B-vitamins

“…As reported in the following paper [Edgar & Macrae, 1937], the chemical and physical properties of the dietary factor present in the yeast fuller's earth filtrate show that this factor is not identical with Gy6rgy's "vitamin B6" [1935,1], which, in his experiments, together with lactoflavin, appeared to provide the whole vitamin B2 complex. It was possible that some supplementary factor for lactoflavin, not contained in the yeast fuller's earth filtrate, was adsorbed with the lactoflavin on the fuller's earth; this paper describes experiments in which treatment of this adsorbate has yielded an additional active, lactoflavin-free fraction which, when fed with lactoflavin and the fuller's earth filtrate, appears to satisfy the whole vitamin B2 requirements of rats.…”

“…EXPERIMENTAL The methods employed in the present work were similar to those described in the preceding paper [Edgar et al 1937], the criterion being growth of young rats. Rats were deprived of the vitamins of the B2 group at weaning and after 10-14 days, when all weight increase had ceased, received the test doses of the different fractions supplemented by 50y daily of lactoflavin, an amount in excess of the optimum requirements [Edgar et al 1937]. Crystalline vitamin B1 hydrochloride was supplied from the time of weaning, the doses being 10-20y daily according to the body weight.…”

“…Comparison of the growth-promoting action of the B-vitamins provided as (a) untreated aqueous yeast extract, (b) yeast extract autoclaved at pH 5, supplemented by crystalline vitamin B1 hydrochloride and (c) the fuller's earth filtrate from autoclaved yeast extract supplemented by vitamin B1 and lactofiavin A litter of rats was divided into 3 groups as follows: 2 males received doses of an untreated aqueous yeast extract equivalent to 1 g. yeast, dry wt. daily; 2 males received similar doses of the yeast extract after autoclaving at pH 5 at 1200 for 5 hr., supplemented by 10-20y daily of vitamin B1 hydrochloride; 2 males and 2 females received doses of the yeast fuller's earth filtrate [see Edgar et al 1937] equivalent to 1-1'5 g. yeast, dry wt. daily, supplemented by 1-20y daily of vitamin B1 and 5S-75y daily of lactoflavin.…”

A new factor in the production and cure of macrocytic anaemias and its relation to other haemopoietic principles curative in pernicious anaemia,

“…1). These experiments demonstrate the activity in the cure of the monkey anaemia of extracts free from vitamin B1 and the flavin fraction of the vitamin B2 complex but containing the yeast fuller's earth filtrate fraction of Edgar & Macrae [1937].…”

“…(d) Preparation of an alcoholic extract of wheat germ. This extract was prepared by the method of Edgar et al [1937] and the extracted material from 600 g. (dry weight 525 g.) dissolved in 200 ml. water.…”