The wave of the SARS-CoV-2 Omicron variant resulted in a rapid spike and decline as highlighted by municipal wastewater surveillance

Cutrupi, Francesca; Cadonna, Maria; Manara, Serena; Postinghel, Mattia; Rosa, Giuseppina La; Suffredini, Elisabetta; Foladori, P.

doi:10.1016/j.eti.2022.102667

Cited by 27 publications

(15 citation statements)

References 45 publications

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“…We validate both this Omicron- and a Delta variant specific assay ( Lee et al, 2021a , 2022 ) in influent samples collected from a WWTP in Italy over a duration of 10 months (April 2021-January 2022). While similar RT-qPCR assays have been developed and applied for Omicron in Italy, it remains a challenge to derive accurate variant trends, in part due to potential assay cross-reactivity against other SARS-CoV-2 variants ( Cutrupi et al, 2022 ; La Rosa et al, 2022 ). Here we show that our assays were able to provide, through wastewater testing, quantitatively accurate trends and reveal variant displacement dynamics that concur with clinical observations.…”

Section: Introductionmentioning

confidence: 99%

Rapid displacement of SARS-CoV-2 variant Delta by Omicron revealed by allele-specific PCR in wastewater

et al. 2022

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Section: Introductionmentioning

confidence: 99%

Rapid displacement of SARS-CoV-2 variant Delta by Omicron revealed by allele-specific PCR in wastewater

et al. 2022

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“…Before viral concentration, we pretreated samples in a water bath at 56°C for 30 min to inactivate the virus and protect laboratory technicians, as per a previous study ( 9 ). We used a polyethylene glycol/sodium chloride precipitation protocol originally developed for SARS-CoV-2 environmental surveillance ( 10 , 11 ) but modified the protocol by increasing the initial wastewater volume to 90 mL (2 tubes of 45 mL) and eluting all the extracted nucleic acids in 50 µL of elution buffer supplied with the kit. We used NucliSens miniMAG (bioMérieux, https://www.biomerieux.com ) semi-automatic extraction platform to extract nucleic acids.…”

Section: The Studymentioning

confidence: 99%

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy

Rosa¹,

Mancini²,

Veneri³

et al. 2023

Emerg. Infect. Dis.

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M onkeypox virus (MPXV), a member of the family Poxviridae, causes monkeypox, a viral zoonosis detected in north Africa in the 1970s (1). MPXV can be transmitted between humans through contact with lesions, body fluids, respiratory droplets, and contaminated materials (1).In May 2022, an epidemic of monkeypox in nonendemic regions outside Africa began receiving worldwide attention. On July 23, 2022, the World Health Organization declared monkeypox a public health emergency of international concern (2), and 24,973 monkeypox cases had been recognized in 45 countries throughout Europe by October 12, 2022 (3).Rapid identification of outbreaks and clusters is critical for infection control. Sewage surveillance has been recognized as a powerful tool for assessing the circulation of pathogens. After the European Union issued Recommendation 2021/472 (http://data.europa. eu/eli/reco/2021/472/oj), wastewater surveillance was successfully used to track SARS-CoV-2 and its variants across EU countries (4). Studies have demonstrated MPXV DNA sheds from saliva, feces, urine, semen, and skin lesions (5-7), suggesting that the viral genome could occur in wastewater. Various research groups involved in SARS-CoV-2 environmental surveillance extended their efforts to investigate MPXV DNA in wastewater. Studies from the Netherlands and western California, USA, have documented successful detection of MPXV DNA in sewage (8; M.K. Wolfe

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“…Different variants of SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) have been identified since the beginning of the COVID-19 pandemic. Some have been designated as variants of concern (VOC), including Omicron ( 1 ). This variant (B.1.1.529 lineage) was reported for the first time in Botswana and South Africa ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

“…Some have been designated as variants of concern (VOC), including Omicron ( 1 ). This variant (B.1.1.529 lineage) was reported for the first time in Botswana and South Africa ( 1 ). Omicron and its sub-lineages have become the dominant circulating strains worldwide, causing an increase in reported cases of COVID-19 at the end of 2022, especially in Japan and South Korea ( 2 , 3 ).…”

Section: Introductionmentioning

confidence: 99%

“…Omicron and its sub-lineages have become the dominant circulating strains worldwide, causing an increase in reported cases of COVID-19 at the end of 2022, especially in Japan and South Korea ( 2 , 3 ). This VOC has been associated with a ttenuated pathogenicity ( 4 ),increased transmissibility, and a greater magnitude of breakthrough infections and reinfections due to distinctive immune evasion mechanisms ( 1 , 4 – 6 ).…”

Section: Introductionmentioning

confidence: 99%

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Neutralizing antibody titers to Omicron six months after vaccination with BNT162b2 in Colombia

et al. 2022

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The emergence of the Omicron variant has generated concerns about the efficacy of COVID-19 vaccines. We evaluated the serum neutralizing activity of antibodies against the Omicron (lineage BA.1.1) by plaque reduction neutralizing test, as well as its correlation with age and gender, in a Colombian cohort six months after being vaccinated with BNT162b2 (Pfizer/BioNTech). Compared to all other variants analyzed, a significantly lower neutralizing activity (p<0.001) was observed against Omicron. Interestingly, older individuals exhibited lower titers against Omicron than those younger than 40. No statistical differences in neutralizing activity were observed according to gender. Our results showed that two doses of BNT162b2 might not provide robust protection against the Omicron variant over time. It is necessary to consider including changes in the composition of the vaccines to protect against new emerging variants of SARS-CoV-2 and campaigns to implement additional booster vaccinations.

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The wave of the SARS-CoV-2 Omicron variant resulted in a rapid spike and decline as highlighted by municipal wastewater surveillance

Cited by 27 publications

References 45 publications

Rapid displacement of SARS-CoV-2 variant Delta by Omicron revealed by allele-specific PCR in wastewater

Rapid displacement of SARS-CoV-2 variant Delta by Omicron revealed by allele-specific PCR in wastewater

Detection of Monkeypox Virus DNA in Airport Wastewater, Rome, Italy

Neutralizing antibody titers to Omicron six months after vaccination with BNT162b2 in Colombia

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