The Weanling Guinea Pig as Test Object for Estrogen Assays

Hartman, Carl G.; Littrell, Jae L.; Tom, John

doi:10.1210/endo-39-2-120

Cited by 13 publications

(4 citation statements)

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“…Previous studies have indicated that resorption of the vaginal membrane of the guinea pig is caused by administration of estrogen and inhibited by progesterone (Hartman et al, 1946;Jagiello, 1965). The vaginal membrane is an epithelial membrane that closes the external opening of the vagina and typically is open or "resorbed" only during estrus and at parturition.…”

Section: Discussionmentioning

confidence: 99%

Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

Larkin¹,

Ogilvie²,

Wubbel³

et al. 1987

Am. J. Anat.

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Guinea pigs were spayed and given various regimens of injections of estradiol and progesterone. The following were monitored in each animal: pubic separation (relaxin stimulation), resorption of the vaginal membrane (estrogen priming), and the presence of PAS-positive granules and/or relaxin in endometrial gland cells (EGC). Injections of estradiol alone resulted in resorption of the vaginal membrane, accumulation of PAS-positive granules in EGC of all animals, and accumulation of relaxin in EGC of two of four animals; but they did not cause pubic separation. Progesterone injections did not result in resorption of the vaginal membrane, separation of pubes, or accumulation of PAS-positive granules; however, one of three animals demonstrated a few EGC that contained relaxin. Animals that received both estradiol and progesterone exhibited PAS-positive granules and relaxin in EGC as well as separated pubes, but did not have resorbed vaginal membranes. Upon examination with the electron microscope, EGC from animals that received estradiol alone exhibited remarkable numbers of secretory granules that contained a carbohydrate-rich material. Secretory granules were not prominent in EGC from animals that received progesterone alone. Estradiol and progesterone injections resulted in accumulation of secretory granules in EGC that contained relaxin and a carbohydrate-rich material. The observations that estradiol and progesterone induce relaxin production in EGC support the hypothesis that uterine relaxin plays an important role in pregnancy and/or parturition in the guinea pig.

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Section: Discussionmentioning

confidence: 99%

Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

Larkin¹,

Ogilvie²,

Wubbel³

et al. 1987

Am. J. Anat.

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“…The most sensitive tests for estrogens thus far proposed are those of Hartman and Littrell (1945), who report that the vagina of the immature rat opens in response to 0.02 cc. of blood from women in the middle of the cycle, and of Hartman, Littrell, and Tom "(1946), who state that as little as 0.0004 jug. estradiol dipropionate will open the vagina of the weanling guinea pig.…”

mentioning

confidence: 99%

A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE¹

1947

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“…In principle the method described by Hartman et al (1946) was used with ovariectomized mature guinea pigs. Each compound was orally administered once daily for 5 days.…”

Section: Animalsmentioning

confidence: 99%

Oestrogenic Activity of Oestradiol-Decanoate After Oral Administration to Rodents

Visser¹,

Vies²

1977

Acta Endocrinologica

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Oestradiol-decanoate, dissolved in arachis oil and orally administered to rodents, produces oestrogenic effects. Compared on a molecular basis the ester has 0.1\p=n-\1.0 times the activity of ethinyl oestradiol, dependent on the species and the parameter studied.The effects of oestradiol-decanoate are less or absent when the oil is omitted. It is likely that absorption of the steroid ester takes place via the intestinal lymphatics in conjunction with the oil. The principal oestrogen, naturally produced by the human ovaries, oestradiol-17/?, is not very effective in bringing about therapeutic oestrogenic effects if it is administered orally.For this reason oestradiol derivatives, such as ethinyl oestradiol and mestranol, are commonly used if substitution therapy with an orally active oestro¬ genic steroid is required, e. g. after ovariectomy or in the post-menopause.However, concern about possible unwanted side effects of such compounds has developed and a preparation which acts, after oral administration, by increasing the level of the natural hormone, oestradiol, in the plasma would be of advantage.The demonstration of the oral androgenic activity of testosterone-undecanoate dissolved in oil, which results from an increase of natural androgens in the plasma ), induced us to study a similar lipophilic ester, oestradiol decanoate, for its oestrogenic effects.In this report the results of this study in rodents are described. 422Endocrinological R&D Laboratories,

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The Weanling Guinea Pig as Test Object for Estrogen Assays

Cited by 13 publications

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Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE¹

Oestrogenic Activity of Oestradiol-Decanoate After Oral Administration to Rodents

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The Weanling Guinea Pig as Test Object for Estrogen Assays

Cited by 13 publications

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Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

Effects of estradiol and progesterone on accumulation of relaxin‐ and carbohydrate‐containing granules in endometrial gland cells of the guinea pig

A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE1

Oestrogenic Activity of Oestradiol-Decanoate After Oral Administration to Rodents

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A BIO-ASSAY FOR MINUTE AMOUNTS OF PROGESTERONE¹