The Wheat Grain Contains Pectic Domains Exhibiting Specific Spatial and Development-Associated Distribution

Chateigner‐Boutin, Anne‐Laure; Bouchet, Brigitte; Alvarado, Camille; Bakan, Bénédicte; Guillon, Fabienne

doi:10.1371/journal.pone.0089620

Cited by 57 publications

(65 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This degradation has been described to be a crucial event for cell wall elongation during the growth and development of A. thaliana seedlings [43]. Recently, pectins (HG and rhamnogalacturonan) have been detected not only in the endosperm and the outer layers of wheat grains but also in the seed coat beneath a thick cuticle [44]. Pectins were also detected by immunolabelling the B. distachyon grain at maturation, but no information is available concerning the occurrence of pectins at earlier developmental stages [4].…”

Section: Discussionmentioning

confidence: 99%

“…Pectins were also detected by immunolabelling the B. distachyon grain at maturation, but no information is available concerning the occurrence of pectins at earlier developmental stages [4]. The higher amount of GH28 at 9 and 13 DAF is consistent with the remodelling of early synthesised pectins in the developing grain [44,45]. In addition, only methylated HG was detected at early stages of development of the wheat grain.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

Self Cite

View full text Add to dashboard Cite

Brachypodium distachyon is a suitable plant model for studying temperate cereal crops, such as wheat, barley or rice, and helpful in the study of the grain cell wall. Indeed, the most abundant hemicelluloses that are in the B. distachyon cell wall of grain are (1-3)(1-4)-β-glucans and arabinoxylans, in a ratio similar to those of cereals such as barley or oat. Conversely, these cell walls contain few pectins and xyloglucans. Cell walls play an important role in grain physiology. The modifications of cell wall polysaccharides that occur during grain development and filling are key in the determination of the size and weight of the cereal grains. The mechanisms required for cell wall assembly and remodelling are poorly understood, especially in cereals. To provide a better understanding of these processes, we purified the cell wall at three developmental stages of the B. distachyon grain. The proteins were then extracted, and a quantitative and comparative LC-MS/MS analysis was performed to investigate the protein profile changes during grain development. Over 466 cell wall proteins (CWPs) were identified and classified according to their predicted functions. This work highlights the different proteome profiles that we could relate to the main phases of grain development and to the reorganization of cell wall polysaccharides that occurs during these different developmental stages. These results provide a good springboard to pursue functional validation to better understand the role of CWPs in the assembly and remodelling of the grain cell wall of cereals.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…3). While pectin has generally been assumed to be absent from endosperm cell walls of barley endosperm and most other cereals (Stone and Fincher, 2004), it has been reported to be found in the walls of rice endosperm (Ishii et al, 1989) and, more recently, in the starchy endosperm walls of wheat, albeit at low levels (Chateigner-Boutin et al, 2014). The differential expression of these genes during cellularization of the barley endosperm suggests that pectin is deposited transiently in the walls or in cell plates during mitotic cell division.…”

Section: Discussionmentioning

confidence: 99%

The Dynamics of Transcript Abundance during Cellularization of Developing Barley Endosperm

et al. 2016

View full text Add to dashboard Cite

“…Small but significant pectic deposits have recently been reported in wheat grain (Chateigner-Boutin et al, 2014). Pectins have previously been reported in rice endosperm cell walls (Shibuya and Nakane, 1984) and in B. distachyon (Guillon et al, 2011) but little is known about their presence or otherwise in the majority of cereal grains.…”

Section: Minor Wall Polysaccharides In the Grainmentioning

confidence: 99%

Evolution and development of cell walls in cereal grains

2014

View full text Add to dashboard Cite

The composition of cell walls in cereal grains and other grass species differs markedly from walls in seeds of other plants. In the maternal tissues that surround the embryo and endosperm of the grain, walls contain higher levels of cellulose and in many cases are heavily lignified. This may be contrasted with walls of the endosperm, where the amount of cellulose is relatively low, and the walls are generally not lignified. The low cellulose and lignin contents are possible because the walls of the endosperm perform no load-bearing function in the mature grain and indeed the low levels of these relatively intractable wall components are necessary because they allow rapid degradation of the walls following germination of the grain. The major non-cellulosic components of endosperm walls are usually heteroxylans and (1,3;1,4)-β-glucans, with lower levels of xyloglucans, glucomannans, and pectic polysaccharides. Pectic polysaccharides and xyloglucans are the major non-cellulosic wall constituents in most dicot species, in which (1,3;1,4)-β-glucans are usually absent and heteroxylans are found at relatively low levels. Thus, the “core” non-cellulosic wall polysaccharides in grain of the cereals and other grasses are the heteroxylans and, more specifically, arabinoxylans. The (1,3;1,4)-β-glucans appear in the endosperm of some grass species but are essentially absent from others; they may constitute from zero to more than 45% of the cell walls of the endosperm, depending on the species. It is clear that in some cases these (1,3;1,4)-β-glucans function as a major store of metabolizable glucose in the grain. Cereal grains and their constituent cell wall polysaccharides are centrally important as a source of dietary fiber in human societies and breeders have started to select for high levels of non-cellulosic wall polysaccharides in grain. To meet end-user requirements, it is important that we understand cell wall biology in the grain both during development and following germination.

show abstract

The Wheat Grain Contains Pectic Domains Exhibiting Specific Spatial and Development-Associated Distribution

Cited by 57 publications

References 46 publications

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

Understanding the Remodelling of Cell Walls during Brachypodium distachyon Grain Development through a Sub-Cellular Quantitative Proteomic Approach

The Dynamics of Transcript Abundance during Cellularization of Developing Barley Endosperm

Evolution and development of cell walls in cereal grains

Contact Info

Product

Resources

About