The wheat growth-promoting traits of Ochrobactrum and Pantoea species, responsible for solubilization of different P sources, are ensured by genes encoding enzymes of multiple P-releasing pathways

Rasul, Maria; Yasmin, Sumera; Yahya, Mahreen; Breitkreuz, Claudia; Tarkka, Mika; Reitz, Thomas

doi:10.1016/j.micres.2021.126703

Cited by 33 publications

(20 citation statements)

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“…The current study reported amplification of PqqE gene in ZW32 and SSR (Figure 3 and Supplementary Table S3). In our previous study (Rasul et al, 2021), expression analysis of multiple genes related to P-release pathways of PSB was carried out using qPCR. This study revealed that Ochrobactrum sp.…”

Section: Discussionmentioning

confidence: 99%

Differential Root Exudation and Architecture for Improved Growth of Wheat Mediated by Phosphate Solubilizing Bacteria

et al. 2021

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Phosphorous (P) deficiency is a major challenge faced by global agriculture. Phosphate-solubilizing bacteria (PSB) provide a sustainable approach to supply available phosphates to plants with improved crop productivity through synergistic interaction with plant roots. The present study demonstrates an insight into this synergistic P-solubilizing mechanism of PSB isolated from rhizosphere soils of major wheat-growing agro-ecological zones of Pakistan. Seven isolates were the efficient P solubilizers based on in vitro P-solubilizing activity (233-365 μg ml–1) with a concomitant decrease in pH (up to 3.5) by the production of organic acids, predominantly acetic acid (∼182 μg ml–1) and gluconic acid (∼117 μg ml–1). Amplification and phylogenetic analysis of gcd, pqqE, and phy genes of Enterobacter sp. ZW32, Ochrobactrum sp. SSR, and Pantoea sp. S1 showed the potential of these PSB to release orthophosphate from recalcitrant forms of phosphorus. Principal component analysis indicates the inoculation response of PSB consortia on the differential composition of root exudation (amino acids, sugars, and organic acids) with subsequently modified root architecture of three wheat varieties grown hydroponically. Rhizoscanning showed a significant increase in root parameters, i.e., root tips, diameter, and surface area of PSB-inoculated plants as compared to uninoculated controls. Efficiency of PSB consortia was validated by significant increase in plant P and oxidative stress management under P-deficient conditions. Reactive oxygen species (ROS)-induced oxidative damages mainly indicated by elevated levels of malondialdehyde (MDA) and H2O2 contents were significantly reduced in inoculated plants by the production of antioxidant enzymes, i.e., superoxide dismutase, catalase, and peroxidase. Furthermore, the inoculation response of these PSB on respective wheat varieties grown in native soils under greenhouse conditions was positively correlated with improved plant growth and soil P contents. Additionally, grain yield (8%) and seed P (14%) were significantly increased in inoculated wheat plants with 20% reduced application of diammonium phosphate (DAP) fertilizer under net house conditions. Thus, PSB capable of such synergistic strategies can confer P biofortification in wheat by modulating root morphophysiology and root exudation and can alleviate oxidative stress under P deficit conditions.

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Section: Discussionmentioning

confidence: 99%

Differential Root Exudation and Architecture for Improved Growth of Wheat Mediated by Phosphate Solubilizing Bacteria

et al. 2021

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“…The primers for the amplification of pqqC genes of Ochrobactrum sp. SSR, encoding proteins involved in inorganic P solubilization were designed according to Rasul et al ( 2021 ). The primers for gcd and pho gene of Ochrobactrum sp.…”

Section: Methodsmentioning

confidence: 99%

“…The primers for gcd and pho gene of Ochrobactrum sp. SSR have been designed and reported in our preceding study (Rasul et al, 2021 ). The primers used in PCR amplification to evaluate the functionality and to authenticate the predicted sizes of amplicons ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

“…SSR and were used in PCR amplifications to verify the amplification of the predicted amplicon sizes ( Table 1 ). The primers for pho gene were designed and reported in the previous study (Rasul et al, 2021 ). The Gcd gene was selected as reference gene to normalize and validate qRT-PCR experiment by screening relative expression (based on fold change values) of candidate genes, i.e., pqqC and pho genes.…”

Section: Methodsmentioning

confidence: 99%

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Designing Synergistic Biostimulants Formulation Containing Autochthonous Phosphate-Solubilizing Bacteria for Sustainable Wheat Production

et al. 2022

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Applying phosphate-solubilizing bacteria (PSB) as biofertilizers has enormous potential for sustainable agriculture. Despite this, there is still a lack of information regarding the expression of key genes related to phosphate-solubilization (PS) and efficient formulation strategies. In this study, we investigated rock PS by Ochrobactrum sp. SSR (DSM 109610) by relating it to bacterial gene expression and searching for an efficient formulation. The quantitative PCR (qPCR) primers were designed for PS marker genes glucose dehydrogenase (gcd), pyrroloquinoline quinone biosynthesis protein C (pqqC), and phosphatase (pho). The SSR-inoculated soil supplemented with rock phosphate (RP) showed a 6-fold higher expression of pqqC and pho compared to inoculated soil without RP. Additionally, an increase in plant phosphorous (P) (2%), available soil P (4.7%), and alkaline phosphatase (6%) activity was observed in PSB-inoculated plants supplemented with RP. The root architecture improved by SSR, with higher root length, diameter, and volume. Ochrobactrum sp. SSR was further used to design bioformulations with two well-characterized PS, Enterobacter spp. DSM 109592 and DSM 109593, using the four organic amendments, biochar, compost, filter mud (FM), and humic acid. All four carrier materials maintained adequate survival and inoculum shelf life of the bacterium, as indicated by the field emission scanning electron microscopy analysis. The FM-based bioformulation was most efficacious and enhanced not only wheat grain yield (4–9%) but also seed P (9%). Moreover, FM-based bioformulation enhanced soil available P (8.5–11%) and phosphatase activity (4–5%). Positive correlations were observed between the PSB solubilization in the presence of different insoluble P sources, and soil available P, soil phosphatase activity, seed P content, and grain yield of the field grown inoculated wheat variety Faisalabad-2008, when di-ammonium phosphate fertilizer application was reduced by 20%. This study reports for the first time the marker gene expression of an inoculated PSB strain and provides a valuable groundwork to design field scale formulations that can maintain inoculum dynamics and increase its shelf life. This may constitute a step-change in the sustainable cultivation of wheat under the P-deficient soil conditions.

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“…The ability of some CF species belonging to the genera Talaromyces, Penicillium, and Purpureocillium as P solubilizers have been reported [42][43][44]. The two main mechanisms involved in P solubilization by microorganisms are the synthesis of phosphatase and phytase enzymes [45][46][47][48] and the release of organic acids such as oxalic, citric, acetic, succinic, and lactic [48][49][50][51] during the decomposition process. Data is represented by the mean±SE (n=3).…”

Section: Phosphorusmentioning

confidence: 99%

Cellulolytic fungi modify the nitrogen, phosphorus, and potassium contents of organic fertilizers

Fikrinda

Marlina

Khalil

2022

IOP Conf. Ser.: Earth Environ. Sci.

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Cellulolytic fungi are involved in releasing nutrients from organic sources. The objective of this study was to determine the effect of cellulolytic fungi on the total nitrogen (N), total phosphorus (P), and total potassium (K) contents in different organic fertilizers. This study was arranged in randomized block design 3 X 3 with three replications. There were two factors, cellulolytic fungi (Talaromyces pinophilus strain MR107, Penicillium sp. isolate SR18, and Purpureocillium lilacinum isolate PIGD23i) and organic fertilizers (cow manure, compost, and vermicompost). The results indicated that Penicillium sp. isolate SR18 had a better effect than other cellulolytic fungi species on tot. N, tot. P and tot. K contents of cow manure; tot. N and tot. K contents of compost; and tot. P content of vermicompost while P. lilacinum isolate PIGD23i showed a better effect on tot. N and tot. K contents of vermicompost. The highest tot. N, and tot. K contents were found in P. lilacinum isolate PIGD23i-inoculated vermicompost while the tot. P content was in Penicillium sp. isolate SR18-inoculated vermicompost. Overall, adding cellulolytic fungi is an alternative way to improve the nutrient content of organic fertilizer.

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The wheat growth-promoting traits of Ochrobactrum and Pantoea species, responsible for solubilization of different P sources, are ensured by genes encoding enzymes of multiple P-releasing pathways

Cited by 33 publications

References 65 publications

Differential Root Exudation and Architecture for Improved Growth of Wheat Mediated by Phosphate Solubilizing Bacteria

Differential Root Exudation and Architecture for Improved Growth of Wheat Mediated by Phosphate Solubilizing Bacteria

Designing Synergistic Biostimulants Formulation Containing Autochthonous Phosphate-Solubilizing Bacteria for Sustainable Wheat Production

Cellulolytic fungi modify the nitrogen, phosphorus, and potassium contents of organic fertilizers

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