The wheat <scp>NB</scp>‐<scp>LRR</scp> gene <i>Ta<scp>RCR</scp>1</i> is required for host defence response to the necrotrophic fungal pathogen <i>Rhizoctonia cerealis</i>

Zhu, Xinna; Lu, Chungui; Du, Liangcheng; Ye, Xingguo; Liu, Xin; Coules, A.; Zhang, Zengyan

doi:10.1111/pbi.12665

Cited by 54 publications

(46 citation statements)

References 65 publications

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“…NBS-LRR proteins function as intracellular receptors that detect pathogen effector proteins directly or indirectly by the recognition of effectorinduced modifications to other host proteins, resulting in a suite of defence responses (Eitas and Dangl, 2010;Li et al, 2015). To date, many NBS-LRR proteins involved in plant disease resistance have been identified (Anderson et al, 1997;Ellis et al, 1999;Feuillet et al, 2003;Hinsch and Staskawicz, 1996;Periyannan et al, 2013;Sanseverino et al, 2013;Shen et al, 2007;Wang et al, 2015;Whitham et al, 1994;Zhu et al, 2017). In cotton, several NBS-LRR proteins involved in Verticillium wilt resistance have been reported previously Zhu et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Effector-triggered activation of R proteins leads to an array of protective responses, including reactive oxygen species (ROS) bursts, salicylic acid (SA) accumulation, pathogenesis-related (PR) gene induction and rapid programmed cell death (PCD), called the hypersensitive response (HR), at the infection site (Kandoth and Mitchum, 2013;Wu et al, 2014), thereby preventing further ingress of the pathogen. Over the past few decades, more than 100 R genes that confer resistance to 122 different pathogens have been cloned and characterized from a diversity of plant species (Anderson et al, 1997;Ellis et al, 1999;Feuillet et al, 2003;Hinsch and Staskawicz, 1996;Periyannan et al, 2013;Sanseverino et al, 2013;Shen et al, 2007;Wang et al, 2015;Whitham et al, 1994;Zhu et al, 2017). R proteins can be classified into several super-families based primarily on the presence of specific conserved structural motifs.…”

Section: Introductionmentioning

confidence: 99%

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The island cotton NBS‐LRR gene GbaNA1 confers resistance to the non‐race 1 Verticillium dahliae isolate Vd991

Short

et al. 2018

Molecular Plant Pathology

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Wilt caused by Verticillium dahliae significantly reduces cotton yields, as host resistance in commercially cultivated Gossypium species is lacking. Understanding the molecular basis of disease resistance in non-commercial Gossypium species could galvanize the development of Verticillium wilt resistance in cultivated species. Nucleotide-binding site leucine-rich repeat (NBS-LRR) proteins play a central role in plant defence against pathogens. In this study, we focused on the relationship between a locus enriched with eight NBS-LRR genes and Verticillium wilt resistance in G. barbadense. Independent virus-induced gene silencing of each of the eight NBS-LRR genes in G. barbadense cultivar Hai 7124 revealed that silencing of GbaNA1 alone compromised the resistance of G. barbadense to V. dahliae isolate Vd991. In cultivar Hai 7124, GbaNA1 could be induced by V. dahliae isolate Vd991 and by ethylene, jasmonic acid and salicylic acid. Nuclear protein localization of GbaNA1 was demonstrated by transient expression. Sequencing of the GbaNA1 orthologue in nine G. hirsutum accessions revealed that all carried a non-functional allele, caused by a premature peptide truncation. In addition, all 10 G. barbadense and nine G. hirsutum accessions tested carried a full-length (∼1140 amino acids) homologue of the V. dahliae race 1 resistance gene Gbve1, although some sequence polymorphisms were observed. Verticillium dahliae Vd991 is a non-race 1 isolate that lacks the Ave1 gene. Thus, the resistance imparted by GbaNA1 appears to be mediated by a mechanism distinct from recognition of the fungal effector Ave1.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The island cotton NBS‐LRR gene GbaNA1 confers resistance to the non‐race 1 Verticillium dahliae isolate Vd991

Short

et al. 2018

Molecular Plant Pathology

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“…To date, numerous NBS‐LRR proteins with roles in mediating plant disease resistance have been identified (Anderson et al ., ; Ellis et al ., ; Feuillet et al ., ; Hinsch and Staskawicz, ; Li et al ., ; Periyannan et al ., ; Sanseverino et al ., ; Shen et al ., ; Wang et al ., ; Whitham et al ., ; Zhu et al ., ). NBS‐LRR proteins generally are composed of tripartite domain architectures, an N‐terminal response domain involved in downstream signalling (CC or TIR are examples), a central molecular switch domain (NB‐ARC, a nucleotide‐binding adaptor shared by the mammalian apoptosis regulator Apaf1, and the Caenorhabditis elegans apoptosis regulator CED4), and a C‐terminal sensor domain‐containing LRRs (Collier and Moffett, ; Van der Biezen and Jones, ; Maekawa et al ., ; Meyers et al ., ; Qi and Innes, ).…”

Section: Introductionmentioning

confidence: 97%

The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt

Wang

Yin

et al. 2019

Molecular Plant Pathology

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Summary Improving genetic resistance is a preferred method to manage Verticillium wilt of cotton and other hosts. Identifying host resistance is difficult because of the dearth of resistance genes against this pathogen. Previously, a novel candidate gene involved in Verticillium wilt resistance was identified by a genome‐wide association study using a panel of Gossypium hirsutum accessions. In this study, we cloned the candidate resistance gene from cotton that encodes a protein sharing homology with the TIR‐NBS‐LRR receptor‐like defence protein DSC1 in Arabidopsis thaliana (hereafter named GhDSC1). GhDSC1 expressed at higher levels in response to Verticillium wilt and jasmonic acid (JA) treatment in resistant cotton cultivars as compared to susceptible cultivars and its product was localized to nucleus. The transfer of GhDSC1 to Arabidopsis conferred Verticillium resistance in an A. thaliana dsc1 mutant. This resistance response was associated with reactive oxygen species (ROS) accumulation and increased expression of JA‐signalling‐related genes. Furthermore, the expression of GhDSC1 in response to Verticillium wilt and JA signalling in A. thaliana displayed expression patterns similar to GhCAMTA3 in cotton under identical conditions, suggesting a coordinated DSC1 and CAMTA3 response in A. thaliana to Verticillium wilt. Analyses of GhDSC1 sequence polymorphism revealed a single nucleotide polymorphism (SNP) difference between resistant and susceptible cotton accessions, within the P‐loop motif encoded by GhDSC1. This SNP difference causes ineffective activation of defence response in susceptible cultivars. These results demonstrated that GhDSC1 confers Verticillium resistance in the model plant system of A. thaliana, and therefore represents a suitable candidate for the genetic engineering of Verticillium wilt resistance in cotton.

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“…Sharp eyespot is one of the most serious diseases for wheat production in different regions around the world [2]. Since late 1990s, sharp eyespot has seriously endangered wheat production in China, resulting in 10%-30% yield losses of wheat [3,4]. Rhizoctonia cerealis, a necrotrophic fungus, is the major pathogen of sharp eyespot in China.…”

Section: Introductionmentioning

confidence: 99%

Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot

Wang

Zhang

2020

IJMS

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Wheat (Triticum aestivum L.) is an important staple crop. Sharp eyespot and common root rot are destructive diseases of wheat. Antimicrobial peptides (AMPs) are small peptides with broad-spectrum antimicrobial activity. In this study, we synthesized the DmAMP1W gene, encoding Dahlia merckii DmAMP1, and investigated the antifungal role of DmAMP1W in vitro and in transgenic wheat. Protein electrophoresis analysis and in vitro inhibition results demonstrated that the synthesized DmAMP1W correctly translated to the expected peptide DmAMP1W, and the purified peptide inhibited growths of the fungi Rhizoctonia cerealis and Bipolaris sorokiniana, the pathogenic causes of wheat sharp eyespot and common root rot. DmAMP1W was introduced into a wheat variety Zhoumai18 via Agrobacterium-mediated transformation. The molecular characteristics indicated that DmAMP1W could be heritable and expressed in five transgenic wheat lines in T1–T2 generations. Average sharp eyespot infection types of these five DmAMP1W transgenic wheat lines in T1–T2 generations decreased 0.69–1.54 and 0.40–0.82 compared with non-transformed Zhoumai18, respectively. Average common root rot infection types of these transgenic lines and non-transformed Zhoumai18 were 1.23–1.48 and 2.27, respectively. These results indicated that DmAMP1W-expressing transgenic wheat lines displayed enhanced-resistance to both sharp eyespot and common root rot. This study provides new broad-spectrum antifungal resources for wheat breeding.

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The wheat NB‐LRR gene TaRCR1 is required for host defence response to the necrotrophic fungal pathogen Rhizoctonia cerealis

Cited by 54 publications

References 65 publications

The island cotton NBS‐LRR gene GbaNA1 confers resistance to the non‐race 1 Verticillium dahliae isolate Vd991

The island cotton NBS‐LRR gene GbaNA1 confers resistance to the non‐race 1 Verticillium dahliae isolate Vd991

The Gossypium hirsutum TIR‐NBS‐LRR gene GhDSC1 mediates resistance against Verticillium wilt

Ecotopic Expression of the Antimicrobial Peptide DmAMP1W Improves Resistance of Transgenic Wheat to Two Diseases: Sharp Eyespot and Common Root Rot

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