The Wisdom of Honeybee Defenses Against Environmental Stresses

Li, Guilin; Zhao, Hang; Liu, Zhenguo; Wang, Hongfang; Xu, Baohua; Guo, Xingqi

doi:10.3389/fmicb.2018.00722

Cited by 51 publications

(43 citation statements)

References 154 publications

(190 reference statements)

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“…Previous studies have shown that different species of honey bees exhibit different anti-oxidant enzyme responses to field-realistic oral exposures pesticides (Apis cerana and Apis dorsata) [16], as well as varying conditions of temperature and humidity (Apis cerana and Apis mellifera) [55]. Further, pesticide-induced oxidative stress, coupled with other stressors, can reduce longevity in honey bees [56]. Thus, reduced longevity resulting from oxidative stress could negatively affect colony population and ultimately compromise colony fitness.…”

Section: Discussionmentioning

confidence: 99%

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

et al. 2020

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Pesticide exposures can have detrimental impacts on bee pollinators, ranging from immediate mortality to sub-lethal impacts. Flupyradifurone is the active ingredient in Sivanto™ and sulfoxaflor is the active ingredient in Transform ®. They are both relatively new insecticides developed with an intent to reduce negative effects on bees, when applied to bee-attractive crops. With the growing concern regarding pollinator health and pollinator declines, it is important to have a better understanding of any potential negative impacts, especially sublethal, of these pesticides on bees. This study reports novel findings regarding physiological stress experienced by bees exposed to field application rates of these two insecticides via a Potter Tower sprayer. Two contact exposure experiments were conducted-a shorter 6hour study and a longer 10-day study. Honey bee mortality, sugar syrup and water consumption, and physiological responses (oxidative stress and apoptotic protein assays) were assessed in bees exposed to Sivanto™ and Transform ® , and compared to bees in control group. For the longer, 10-day contact exposure experiment, only the Sivanto™ group was compared to the control group, as high mortality recorded in the sulfoxaflor treatment group during the shorter contact exposure experiment, made the latter group unfeasible to test in the longer 10-days experiment. In both the studies, sugar syrup and water consumptions were significantly different between treatment groups and controls. The highest mortality was observed in Transform ® exposed bees, followed by the Sivanto™ exposed bees. Estimates of reactive oxygen/nitrogen species indicated significantly elevated oxidative stress in both pesticide treatment groups, when compared to controls. Caspase-3 protein assays, an indicator of onset of apoptosis, was also significantly higher in the pesticide treatment groups. These differences were largely driven by post exposure duration, indicating sublethal impacts. Further, our findings also emphasize the need to revisit contact exposure impacts of Sivanto™, given the sub-lethal impacts and mortality observed in our long-term (10-day) contact exposure experiment.

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Section: Discussionmentioning

confidence: 99%

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

et al. 2020

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“…Furthermore, in the honeybee, workers may act as ‘heat shields’, positioning themselves on the hottest parts of the hive's walls to protect the brood from overheating (Starks & Gilley, 1999). Additionally, the proportion of workers serving as water foragers can increase (Bordier et al ., 2017), and they may spread water around the comb, allowing evaporative cooling (Li et al ., 2018). Evaporative cooling is also used by a paper wasp ( Polistes biglumis ): workers have been seen to regurgitate droplets of fresh water into nest cells so as to prevent overheating (Fig.…”

Section: Behavioural Adaptationsmentioning

confidence: 99%

Adaptations to thermal stress in social insects: recent advances and future directions

Perez

2020

Biological Reviews

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Thermal stress is a major driver of population declines and extinctions. Shifts in thermal regimes create new environmental conditions, leading to trait adaptation, population migration, and/or species extinction. Extensive research has examined thermal adaptations in terrestrial arthropods. However, little is known about social insects, despite their major role in ecosystems. It is only within the last few years that the adaptations of social insects to thermal stress have received attention. Herein, we discuss what is currently known about thermal tolerance and thermal adaptation in social insects – namely ants, termites, social bees, and social wasps. We describe the behavioural, morphological, physiological, and molecular adaptations that social insects have evolved to cope with thermal stress. We examine individual and collective responses to both temporary and persistent changes in thermal conditions and explore the extent to which individuals can exploit genetic variability to acclimatise. Finally, we consider the costs and benefits of sociality in the face of thermal stress, and we propose some future research directions that should advance our knowledge of individual and collective thermal adaptations in social insects.

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“…In agricultural production, various pesticides and herbicides are often used to increase crop yields. The use of herbicides reduces the diversity of the food resources of honeybees, and insecticides directly affect the immunity and antioxidant capacity of bees (Sánchez‐Bayo et al ., 2016; Li et al ., 2018a). Their extensive use has caused obvious damage to honeybees (Tsvetkov et al ., 2017).…”

Section: Discussionmentioning

confidence: 99%

Identification of a mitogen‐activated protein kinase kinase (AccMKK4) from Apis cerana cerana and its involvement in various stress responses

Guo

Zhao

et al. 2021

Insect Molecular Biology

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The mitogen‐activated protein kinase (MAPK) cascade pathway is a ubiquitous signal transduction pathway in eukaryotes that regulates a variety of immune responses. This study accomplished the first isolation of an AccMKK4 gene from Apis cerana cerana and explored its function. Yeast two‐hybrid experiments proved that AccMKK4 can interact with Accp38b, and the silencing of AccMKK4 in honeybees downregulated the expression level of Accp38b, which suggests that AccMKK4 might participate in the oxidative stress response through the p38 MAPK pathway. Tissue‐specific expression levels of AccMKK4 analysis showed that AccMKK4 in the thorax, particularly muscle tissue, was higher than that in other tissues. The qRT‐PCR results from different conditions demonstrated that AccMKK4 responds to various environmental stresses. After AccMKK4 silencing, the transcription level of some antioxidant genes and the activity of antioxidant‐related enzymes are reduced, which indicated that AccMKK4 plays an important role in resistance against oxidative stress caused by external stimuli. In summary, our findings indicate that AccMKK4 probably plays an indispensable role in the response of honeybees to environmental stress and might aid for further research on the role of the MAPK cascade pathway in the antioxidant defence mechanisms of insects.

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The Wisdom of Honeybee Defenses Against Environmental Stresses

Cited by 51 publications

References 154 publications

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

Field rates of Sivanto™ (flupyradifurone) and Transform® (sulfoxaflor) increase oxidative stress and induce apoptosis in honey bees (Apis mellifera L.)

Adaptations to thermal stress in social insects: recent advances and future directions

Identification of a mitogen‐activated protein kinase kinase (AccMKK4) from Apis cerana cerana and its involvement in various stress responses

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