The Wnt modulator ICG‑001 mediates the inhibition of nasopharyngeal carcinoma cell migration in vitro via the miR‑150/CD44 axis

Chan, Lai-Sheung; Man, On‑Ying; Kwok, Hoi‐Hin; Luo, Chen; Chan, Kwok-shing; Lung, Hong-Lok; Ngan, Roger K.C.; Wong, Ricky N S; Lo, Kwok‐Wai; Lee, Anne W.M.; Tsao, George Sai‐Wah; Kahn, Michaël; Lung, Maria Li; Mak, Nai‐Ki

doi:10.3892/ijo.2018.4664

Cited by 13 publications

(23 citation statements)

References 45 publications

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“…However, the regulation of expression and also the biological actions of EVI1 in NPC cells are still unknown. We have previously demonstrated that the small specific molecule CBP/β-catenin antagonist ICG-001 can inhibit the migration and growth of CSC-enriched NPC tumor spheres via the miRNA-145/SOX2 (SRY-Box Transcription Factor 2) axis [22] and miR-150/CD44 axis [23]. In the present study, we further examined the underlying antitumor mechanisms of ICG-001 in NPC cells.…”

Section: Introductionmentioning

confidence: 84%

“…Apart from the miR-449a, we have previously demonstrated that ICG-001 could also inhibit the growth of CSC-enriched NPC tumor spheroids via miR-145/SOX2 axis [22] and the NPC cell migration via miR-150/CD44 axis [23]. Figure 8 summarizes the hypothetical mode of action of ICG-001 on NPC cells.…”

Section: Discussionmentioning

confidence: 99%

“…Cell migration assay was performed as described previously [ 23 ]. Briefly, transfected C666-1 cells were seeded onto 6.5 mm transwell inserts with an 8.0 μm pore polycarbonate membrane (Corning Incorporated, Corning, NY, USA).…”

Section: Methodsmentioning

confidence: 99%

“…The EBV-positive (C666-1 and C17) and EBV-negative (HONE-1) NPC cell lines were cultured as previously described [22,23]. C666-1 and HONE-1 cell lines were authenticated by and obtained from the Hong Kong NPC AoE Cell Line Repository.…”

Section: Cell Culture and Chemicalsmentioning

confidence: 99%

“…NPC cells were transfected with siRNA or miRNA as previously described [22,23]. Briefly, NPC cells (3 × 10 5 ) were cultured in fibronectin (6 µg/mL) pre-coated 35 mm culture plates overnight.…”

Section: Cell Transfectionmentioning

confidence: 99%

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Role of miR-96/EVI1/miR-449a Axis in the Nasopharyngeal Carcinoma Cell Migration and Tumor Sphere Formation

Chan

Lung

Ngan

et al. 2020

IJMS

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The Wnt signaling pathway is one of the major signaling pathways used by cancer stem cells (CSC). Ecotropic Viral Integration Site 1 (EVI1) has recently been shown to regulate oncogenic development of tumor cells by interacting with multiple signaling pathways, including the Wnt signaling. In the present study, we found that the Wnt modulator ICG-001 could inhibit the expression of EVI1 in nasopharyngeal carcinoma (NPC) cells. Results from loss-of-function and gain-of-function studies revealed that EVI1 expression positively regulated both NPC cell migration and growth of CSC-enriched tumor spheres. Subsequent studies indicated ICG-001 inhibited EVI1 expression via upregulated expression of miR-96. Results from EVI1 3′UTR luciferase reporter assay confirmed that EVI1 is a direct target of miR-96. Further mechanistic studies revealed that ICG-001, overexpression of miR-96, or knockdown of EVI1 expression could restore the expression of miR-449a. The suppressive effect of miR-449a on the cell migration and tumor sphere formation was confirmed in NPC cells. Taken together, the miR-96/EVI1/miR-449a axis is a novel pathway involved in ICG-001-mediated inhibition of NPC cell migration and growth of the tumor spheres.

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Section: Introductionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Cell Culture and Chemicalsmentioning

confidence: 99%

Section: Cell Transfectionmentioning

confidence: 99%

See 3 more Smart Citations

Role of miR-96/EVI1/miR-449a Axis in the Nasopharyngeal Carcinoma Cell Migration and Tumor Sphere Formation

Chan

Lung

Ngan

et al. 2020

IJMS

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Taking the road less traveled – the therapeutic potential of CBP/β-catenin antagonists

Kahn

2021

Expert Opinion on Therapeutic Targets

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MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)

Zhou

Huang

et al. 2021

Bioengineered

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Nasopharyngeal cancer is a rare cancer type, but with a low five-year survival rate. Dysregulation of pyrroline-5-carboxylate reductase 1 (PYCR1) and microRNA hsa-miR-150-5p is involved in the development of various cancers. However, the molecular mechanism of the hsa-miR-150-5p-PYCR1 axis in nasopharyngeal cancer remains unclear. To identify the mechanism of the hsa-miR-150-5p-PYCR1 axis, the expression of hsa-miR-150-5p and PYCR1 in nasopharyngeal cancer tissues and cells was first measured by reverse transcription quantitative polymerase chain reaction. The luciferase and RNA pull-down assays were used to confirm the interaction between hsa-miR-150-5p and PYCR1. The overexpression of hsa-miR-150-5p and PYCR1 was detected by cell viability, proliferation, western blotting, migration, and invasion in nasopharyngeal cancer cells. The expression levels of hsa-miR-150-5p was reduced in the nasopharyngeal cancer tissues and cells and were negatively correlated with the PYCR1 levels. The upregulation of hsa-miR-150-5p significantly repressed cell growth and promoted apoptosis. However, the upregulation of PYCR1 expression significantly promoted nasopharyngeal carcinogenesis, which could abolish the inhibitory effect of hsa-miR-150-5p. In conclusion, we clarified that hsa-miR-150-5p attenuated nasopharyngeal carcinogenesis by reducing the PYCR1 expression levels. This provides a new perspective of nasopharyngeal cancer involving both hsa-miR-150-5p and PYCR1 for the treatment of nasopharyngeal cancer.

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The Wnt modulator ICG‑001 mediates the inhibition of nasopharyngeal carcinoma cell migration in vitro via the miR‑150/CD44 axis

Abstract: The Wnt modulator ICG-001 mediates the inhibition of The Wnt modulator ICG 001 mediates the inhibition of nasopharyngeal carcinoma cell migration in vitro via the miR-150/ nasopharyngeal carcinoma cell migration in vitro via the miR 150/ CD44 axis CD44 axis

Cited by 13 publications

References 45 publications

Role of miR-96/EVI1/miR-449a Axis in the Nasopharyngeal Carcinoma Cell Migration and Tumor Sphere Formation

Role of miR-96/EVI1/miR-449a Axis in the Nasopharyngeal Carcinoma Cell Migration and Tumor Sphere Formation

Taking the road less traveled – the therapeutic potential of CBP/β-catenin antagonists

MicroRNA hsa-miR-150-5p inhibits nasopharyngeal carcinogenesis by suppressing PYCR1 (pyrroline-5-carboxylate reductase 1)

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