The World of Epithelial Sheets

Honda, Hiroaki; Nagai, Tatsuzo

doi:10.1007/978-981-19-2916-8_7

Cited by 1 publication

(1 citation statement)

References 37 publications

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“…Wound healing is an essential mechanism that allows an epithelial tissue to maintain its barrier function (Honda, 2017; Martini et al ., 2017). As a first step, cells must be alerted to the presence of a nearby wound (Stevens and Page-McCaw, 2012; Enyedi and Niethammer, 2015; Park et al ., 2017).…”

Section: Introductionmentioning

confidence: 99%

A mathematical model of calcium signals around laser-induced epithelial wounds

Stevens

O’Connor

Pumford

et al. 2022

Preprint

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Cells around epithelial wounds must first become aware of the wound in order to initiate the wound healing process. An initial response to an epithelial wound is an increase in cytosolic calcium followed by complex calcium signaling events. While these calcium signals are driven by both physical and chemical wound responses, cells around the wound will all be equipped with the same cellular components to produce and interact with the calcium signals. Here, we have developed a mathematical model in the context of laser-ablation of the Drosophila pupal notum that integrates tissue-level damage models with a cellular calcium signaling toolkit. The model replicates experiments in the contexts of control wounds as well as knockdowns of specific cellular components, but it also provides new insights that are not easily accessible experimentally. The model suggests that cell-cell variability is necessary to produce calcium signaling events observed in experiments, it quantifies calcium concentrations during wound-induced signaling events, and it shows that intercellular transfer of the molecule IP3 is required to coordinate calcium signals across distal cells around the wound. The mathematical model developed here serves as a framework for quantitative studies in both wound signaling and calcium signaling in the Drosophila system.

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