The World’s oldest living grapevine specimen and its genetic relationships

Vršič, S.; Ivančič, Anton; Šušek, Andrej; Zagradišnik, Boris; Valdhuber, J.; Šiško, Metka

doi:10.5073/vitis.2011.50.167-171

VITIS - Journal of Grapevine Research 2015

DOI: 10.5073/vitis.2011.50.167-171

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The World’s oldest living grapevine specimen and its genetic relationships

S. Vršič¹,

Anton Ivančič²,

Andrej Šušek³

et al.

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2017

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Cited by 2 publications

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The study of antioxidants in grapevine seeds

Tomášková

Sochor

Baroň

2017

Potr. S. J. F. Sci.

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Grapevine seeds contain a large amount of antioxidant components, and are therefore recommended in the prevention and treatment of many diseases. For this research, we studied the antioxidant properties of grapevine seeds from the Marlen variety, as evidence suggests that these types have higher resistance against fungal diseases. Through high-performance liquid chromatography with UV/VIS detection, a total of 10 antioxidant components were selected for further investigation, specifically: catechin, epicatechin, rutin, quercitrin, quercetin, caftaric acid, caffeic acid, p-coumaric acid, ferulic acid, and gallic acid. The antioxidant activity was determinated spectrophotometrically through the adoption of three fundamentally different methods (the DPPH assay, the ABTS method, and the FRAP method). Using the Folin-Ciocalteu method, it was possible to determine the content of all the polyphenolic compounds. The results of the assessment antioxidant activity and the content of polyphenolic compounds were recalculated to gallic acid equivalents (GAE). The values of the antioxidant activity as determinated by the DPPH test were 6643 (±154) mg of GAE; 1984 (±88) mg of GAE when using the FRAP method; and 812 (±31) mg of GAE when the ABTS method was utilised. The content of the total polyphenolic compounds came to 6982 (±221) mg of GAE. The most abundant antioxidant was catechin, with a content of 115 mg.L -1 , whilst the least represented compound was ferulic acid (0.139 mg.L -1 ). Overall, this study showed a high antioxidant potential of grapevine seeds.

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The study of antioxidants in grapevine seeds

Tomášková

Sochor

Baroň

2017

Potr. S. J. F. Sci.

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Assesment of the antioxidant activity and content of polyphenolic compounds in grapevine seeds

Tomášková

Sochor

Baroň

2017

Potr. S. J. F. Sci.

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Our work was focused on the study of the antioxidant properties of grapevine seeds. We monitored the grapevine seeds of 6 cultivars of Vitis vinifera, L. (Nativa, Kofranka, Blaufränkish, Marlen, Cabernet Moravia and Italian Riesling). Antioxidant activity was determined by three principally different methods (DPPH, ABTS and FRAP), the content of the total polyphenolic compounds was determined by the Folin ciocalteu method, and the content of the total flavanols was determined by DMACA reagent (p-dimethylaminocinnamaldehyde). Results are presented as an equivalent of gallic acid in g.L -1 , respectively were expressed as g.L -1 of catechin equivalents (DMACA method). The highest values of antioxidant activity were measured in the cultivar Nativa (DPPH -7.75 g.L -1 , ABTS -4.888 g.L -1 , FRAP -4.25 g.L -1 ). Conversely, the lowest values of antioxidant activity were detected in the cultivar Kofranka (DPPH -7.08 g.L -1 , ABTS -4.17 g.L -1 , FRAP -4.55 g.L -1 ). Cultivar Nativa also reached the highest content of flavonols (3.77 g.L -1 ). The highest measured values of the content of total polyphenolic compounds were identified in the cultivar Cabernet Moravia (15.2 g.L -1 of GAE). Conversely, the lowest values of the content of total polyphenolic compounds were detected in the cultivar Nativa (8.04 g.L -1 ). Pearson correlation coefficients were calculated for the existing values between antioxidant activity (DPPH, ABTS, FRAP), contents of flavonols, and contents of total polyphenols. The highest correlation coefficient was found between the DPPH and ABTS methods; specifically, it was 0.857.

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The World’s oldest living grapevine specimen and its genetic relationships

Cited by 2 publications

References 9 publications

The study of antioxidants in grapevine seeds

The study of antioxidants in grapevine seeds

Assesment of the antioxidant activity and content of polyphenolic compounds in grapevine seeds

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