The worldwide emergence of plasmid-mediated quinolone resistance

Robicsek, Ari; Jacoby, George A.; Hooper, David C.

doi:10.1016/s1473-3099(06)70599-0

Cited by 828 publications

(722 citation statements)

References 65 publications

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“…26 In this study, the detection of qnrA and aac(6')-Ib-cr associated with the reduced susceptibility to fluoroquinolones showed that these genes are spreading among clinical isolates in Nigeria. Previously, these genes have been reported in some Enterobacteriaceae in Nigeria, together with the qnrD gene variant which was not detected in this study.…”

Section: Conjugation and Curing Assaysmentioning

confidence: 99%

“…Studies have shown that they produce a low level resistance, but facilitate a higher level resistance in association with chromosomal mutations in bacteria harbouring them. 23,24,25 The aac(6')-Ib-cr, which encode a variant of aminoglycoside transferase that confers reduced susceptibility to ciprofloxacin and norfloxacin by N-acetylation of their piperazinyl amine, causes an increase in their MIC two to four fold 26 and qepA gene, which encodes quinolone-specific efflux pump 27 initiate decreased susceptibility to quinolones by up to 64-fold. 28 Resistance to aminoglycosides such as gentamicin, amikacin and tobramycin, have been attributed to the aminoglycoside-modifying enzymes.…”

Section: Introductionmentioning

confidence: 99%

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Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

et al. 2017

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Background: Globally, and particularly in developing countries, the menace of anti-microbial resistance is an accelerating problem. In Nigeria, increase in bacterial resistance has been phenotypically established but due to high cost, few molecular studies have been reported. Objectives: This study screened for presence of transferable resistance genes and mobile genetic elements (MGEs) such as integron among multi-drug resistant (MDR) P. mirabilis. Methods: A total of 108 P. mirabilis strains collected from five tertiary hospitals in SouthWest Nigeria were subjected to antibiotic susceptibility study using disc-diffusion method. Transferable resistance genes and MGEs were amplified using Polymerase chain reaction (PCR) analysis and amplicons sequenced. Results: Varied resistance was observed against all the antibiotics tested. About 56% of the isolates were MDR including those from 0-12 years old children. PCR analysis revealed the presence of aac(6')-Ib (33.3%), plasmid mediated quinolone resistance (PMQR) genes [qnrA (36.7%), acc(6')-Ib-cr (5%)], TEM (48.3%), CTX-M (6.7%) and integrons class 1 (58.3%) and class 2 (26.7%). Sequencing analysis revealed bla TEM-1 , bla CTX-M-15 associated with ISEcp1 and eight different arrays of gene cassettes: aadA1, aadA1-qacH, aadB-aadA2, aadA5, dfrA7, dfrA15, dfrA17, dfrA17-aadA5. Conclusion: Transferable resistance genes in association with MGEs are present in Nigerian P. mirabilis thus their potential in disseminating resistance.

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Section: Conjugation and Curing Assaysmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

et al. 2017

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“…In this study, five of the seven transconjugants harbouring aac(69)-Ib-cr showed no elevation of ciprofloxacin MIC. Indeed, aac(69)-Ib-cr alone confers a degree of resistance sufficiently low that the MIC might not distinguish organisms that carry it from those that do not (Robicsek et al, 2006). Recently, Harajly et al (2010) described transconjugants with no elevation of MIC after the transfer of aac(69)-Ib-cr.…”

Section: A Barguigua and Others 1348mentioning

confidence: 99%

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Barguigua

Otmani

Talmi

et al. 2011

Journal of Medical Microbiology

104

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Of 803 community Escherichia coli (n5767) and Klebsiella pneumoniae (n536) isolates collected from patients with urinary tract infections in three Moroccan cities, 10 E. coli (1.3%) and 2 K. pneumoniae (5.6 %) isolates were shown to produce extended-spectrum b-lactamases (ESBLs). PFGE revealed that the E. coli isolates comprised seven distinct genotypes. The presence of plasmids in the 12 isolates was revealed by conjugation experiments of plasmids from these Enterobacteriaceae strains with E. coli K 12 J 5 , with further isolation of the plasmids in the transconjugants. Subsequent nucleotide sequencing indicated that the plasmids encoded the bla CTX-M , bla OXA , bla TEM and bla SHV genes, including genes for CTX-M-15 (n511), OXA-1 (n511), TEM-1b (n54), SHV-5 (n51) and SHV-1 (n52). Identification of plasmid-mediated quinolone-resistance genes was performed by PCR. The aac(69)Ib-cr variant was detected in all strains, and two strains co-expressed qnrS1, bla CTX-M-15 and bla OXA-1 genes. The presence of ESBLs in the Enterobacteriaceae strains studied was probably due to the dissemination of resistance plasmids with the predominant genotype of bla CTX-M-15.

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“…nalidixic acid or oxolinic acid) was used in humans to treat uncomplicated urinary tract infections caused mainly by E. coli (7). The addition of a fluorine atom and other components to the basic quinolone structure in the 1980s increased the effectiveness and range of action of these antimicrobials to involve other microorganisms (8). The first original veterinary fluorinated quinolone was enrofloxacin, introduced into practice in the Czech Republic in 1992 (7,9).…”

Section: Introductionmentioning

confidence: 99%

Quinolone-resistant Escherichia coli in Poultry Farming

Hricová¹,

Röderová²,

Pudová³

et al. 2017

Cent Eur J Public Health

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SUMMARYIncreasing bacterial resistance to quinolone antibiotics is apparent in both humans and animals. For humans, a potential source of resistant bacteria may be animals or their products entering the human food chain, for example poultry. Between July 2013 and September 2014, samples were collected and analyzed in the Moravian regions of the Czech Republic to isolate the bacterium Escherichia coli. As a result, 212 E. coli isolates were obtained comprising 126 environmental isolates from poultry houses and 86 isolates from cloacal swabs from market-weight turkeys. Subsequently, the E. coli isolates were tested for susceptibility to selected antibiotics. Resistance of the poultry isolates to quinolones ranged from 53% to 73%. Additionally, the presence of plasmid-mediated resistance genes was studied. The genes were confirmed in 58% of the tested strains. The data on resistance of isolates from poultry were compared with results of resistance tests in human isolates obtained in the same regions.The high levels of resistance determined by both phenotyping and genotyping methods and reported in the present study confirm the fact that the use of fluoroquinolones in poultry should be closely monitored.

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The worldwide emergence of plasmid-mediated quinolone resistance

Cited by 828 publications

References 65 publications

Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

Molecular screening of antibiotic-resistant determinants among multidrug-resistant clinical isolates of <i>Proteus mirabilis</i> from SouthWest Nigeria

Characterization of extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae isolates from the community in Morocco

Quinolone-resistant Escherichia coli in Poultry Farming

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