The WUSCHEL-related homeobox1 gene of cucumber regulates reproductive organ development

Niu, Huanhuan; Liu, Xiaofeng; Cui, Tong; Hu, Wang; Li, Sen; Lü, Li; Pan, Yupeng; Zhang, Xiaolan; Weng, Yiqun; Li, Zheng

doi:10.1093/jxb/ery329

Cited by 23 publications

(29 citation statements)

References 62 publications

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“…Expression pattern analysis of these CsWOX genes showed that a few of them were mainly expressed in specific organs, indicating that these genes may be involved in different development processes. For example, CsWOX1a mutation can lead to a mango fruit in cucumber 18 , which is consistent with that it was mainly enriched in fruit (Fig. 4A).…”

Section: Discussionsupporting

confidence: 88%

“…bers in cucumber, we performed BLASTp searches using the full-length sequences of 15 Arabidopsis AtWOX members and analyzed homeodomain using SMART software 11 . Consistent with previous study 18 , 11 putative CsWOX genes were identified in cucumber (Table 1). These genes encoded proteins ranging from 130 and 387AA (amino acids) while isoelectric point (pI) values ranging from 5.54 to 10.08, and the molecular weights from 14.66 to 43.64 kDa (Table 1).…”

Section: Identification and Phylogenetic Analysis Of Cswox Genes In Csupporting

confidence: 90%

“…3A). Interestingly, another previous study also identified the same CsWOX members in cucumber using the homeodomain sequences of AtWOX members for BLASTp searches 18 , indicating that the homeodomain is highly conserved in WOX members.…”

Section: Discussionmentioning

confidence: 80%

“…Many growth and development processes affect cucumber quality and yield. Although WOX members have been shown to regulate many aspects of development in several plant species, only CsWOX1 was reported to regulate early reproductive organ development in cucumber 18 . It is unknown whether CsWOX members regulate different biological processes in cucumber.…”

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confidence: 99%

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Genome-wide analysis of CsWOX transcription factor gene family in cucumber (Cucumis sativus L.)

Song

Liu

et al. 2020

Sci Rep

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WUSCHEL-related homeobox (WOX) transcription factors are plant-specific members that characterized by the presence of a homeodomain. it has been shown that WoX members regulate several aspects of plant development, but the biological functions of this CsWOX gene family remain largely unknown in cucumber (Cucumis sativus L.). In this study, we identified and characterized 11 putative CsWOX genes in cucumber, which are also divided into three major clades (e.g., the Ancient clade, the Intermediate clade and the WUS clade). Expression pattern analysis revealed tissue-specific expression patterns of CsWOX genes, including that CsWOX9 is mainly expressed in developing fruit and also has lower expression in tip and axillary bud, which was further confirmed by in situ hybridization assay. Moreover, overexpression of CsWOX9 in Arabidopsis led to increased branches and rosette leaves, and shorter siliques. together, these results indicated that csWoX members may regulate cucumber growth and development. WUSCHEL-related homeobox (WOX) gene family are plant-specific transcription factors, which belong to homeobox superfamily. All WOX members contain a homeodomain with 60-66 amino acid residues. According to evolutionary relationships, they can be divided into WUS, Intermediate and Ancient clades 1-3. These WOX members have been identified and studied in many species including Arabidopsis, rice, maize and soybean. So far, it has been found that WOX transcription factors play important roles in regulating many aspects of growth and development including meristematic stem cell maintenance, embryonic development and polarization, lateral organ development, and organ regeneration. The founding member AtWUS is expressed in stem apical meristem (SAM) and is necessary for SAM formation and maintenance 2,4-6. In addition to AtWUS, other WOX members are also involved in stem cell maintenance. For example, AtWOX5 is enriched in the quiescent center and plays an important role in maintaining the stability of stem cells of root apical meristem, similar to AtWUS in SAM 7. WOX4 is mainly expressed in the procambium and cambium, and promotes differentiation and/or maintenance of the vascular procambium in Arabidopsis and tomato 8,9. WOX9 promotes the growth of SAM and is required for the maintenance of WUS expression at the shoot apex, and mediates cytokinin signaling during shoot meristem establishment 10. The involvement of WOX members in plant development is not limited to stem cell maintenance. For example, AtWOX2 contributes to early pre-embryo and cotyledon boundary formation during embryo development in the egg cell and zygote 11. Consistently, PaWOX2 also likely regulates embryo cell division and/or differentiation in Picea abies 12. AtWOX7 is expressed at all stages of lateral root development, and inhibits lateral root development in a sugar-dependent manner 13. Similarly, OsWOX6 and OsWOX11 are expressed asymmetrically in response to auxin control rice tiller angle 14. Moreover, AtWOX11 and AtWOX12 play important roles in de nov...

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Section: Discussionsupporting

confidence: 88%

Section: Identification and Phylogenetic Analysis Of Cswox Genes In Csupporting

confidence: 90%

Section: Discussionmentioning

confidence: 80%

mentioning

confidence: 99%

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Genome-wide analysis of CsWOX transcription factor gene family in cucumber (Cucumis sativus L.)

Song

Liu

et al. 2020

Sci Rep

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“…1a ) 27 . We previously identified the cucumber mutant mango fruit ( mf ), which displays multiple flower growth defects as well as male and female sterility 28 . Map-based cloning revealed that a truncated protein lacking the conserved WUS box of WOX1-type transcriptional regulator (CsWOX1) was responsible for the mutant phenotype 28 .…”

Section: Resultsmentioning

confidence: 99%

WUSCHEL-related homeobox1 (WOX1) regulates vein patterning and leaf size in Cucumis sativus

Wang

Niu

et al. 2020

Hortic Res

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In plants, WUSCHEL-related homeobox1 (WOX1) homologs promote lamina mediolateral outgrowth. However, the downstream components linking WOX1 and lamina development remain unclear. In this study, we revealed the roles of WOX1 in palmate leaf expansion in cucumber (Cucumis sativus). A cucumber mango fruit (mf) mutant, resulting from truncation of a WOX1-type protein (CsWOX1), displayed abnormal lamina growth and defects in the development of secondary and smaller veins. CsWOX1 was expressed in the middle mesophyll and leaf margins and rescued defects of the Arabidopsis wox1 prs double mutant. Transcriptomic analysis revealed that genes involved in auxin polar transport and auxin response were highly associated with leaf development. Analysis of the cucumber mf rl (round leaf) double mutant revealed that CsWOX1 functioned in vein development via PINOID (CsPID1)-controlled auxin transport. Overexpression of CsWOX1 in cucumber (CsWOX1-OE) affected vein patterning and produced ‘butterfly-shaped’ leaves. CsWOX1 physically interacted with CsTCP4a, which may account for the abnormal lamina development in the mf mutant line and the smaller leaves in the CsWOX1-OE plants. Our findings demonstrated that CsWOX1 regulates cucumber leaf vein development by modulating auxin polar transport; moreover, CsWOX1 regulates leaf size by controlling CIN-TCP genes.

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Molecular Mapping of QTLs and Genes for Plant Architecture and Fruit Traits in Cucumber

Gebretsadik

2021

Compendium of Plant Genomes

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The WUSCHEL-related homeobox1 gene of cucumber regulates reproductive organ development

Cited by 23 publications

References 62 publications

Genome-wide analysis of CsWOX transcription factor gene family in cucumber (Cucumis sativus L.)

Genome-wide analysis of CsWOX transcription factor gene family in cucumber (Cucumis sativus L.)

WUSCHEL-related homeobox1 (WOX1) regulates vein patterning and leaf size in Cucumis sativus

Molecular Mapping of QTLs and Genes for Plant Architecture and Fruit Traits in Cucumber

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