The X as Model for RNA's Niche in Epigenomic Regulation

Lee, Jeannie T.

doi:10.1101/cshperspect.a003749

Cited by 70 publications

(53 citation statements)

References 125 publications

(169 reference statements)

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“…Finally, long noncoding RNAs usually acting in cis (on the chromosome or the local region where they were synthesized) can turn off gene expression by attracting proteins that modify chromatin structure. The effect can spread to an entire chromosome, in the case of the Xist RNA that condenses one of the two X chromosomes in female mammals and thereby gives gene dosage compensation (Lee 2010). In other cases, the effect is more local, affecting transcription of a single gene or a group of genes (Wang et al 2010).…”

Section: The Contemporary Rna Worldmentioning

confidence: 99%

The RNA Worlds in Context

Cech

2011

Cold Spring Harbor Perspectives in Biology

188

136

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SUMMARYThere are two RNAworlds. The first is the primordial RNAworld, a hypothetical era when RNA served as both information and function, both genotype and phenotype. The second RNA world is that of today's biological systems, where RNA plays active roles in catalyzing biochemical reactions, in translating mRNA into proteins, in regulating gene expression, and in the constant battle between infectious agents trying to subvert host defense systems and host cells protecting themselves from infection. This second RNA world is not at all hypothetical, and although we do not have all the answers about how it works, we have the tools to continue our interrogation of this world and refine our understanding. The fun comes when we try to use our secure knowledge of the modern RNAworld to infer what the primordial RNAworld might have looked like.

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Section: The Contemporary Rna Worldmentioning

confidence: 99%

The RNA Worlds in Context

Cech

2011

Cold Spring Harbor Perspectives in Biology

188

136

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“…Many groups have reported that ncRNAs recruit the PRC2 complex and direct silencing to relevant regions of the chromosome. Extensive studies on the roles of ncRNA in X inactivation center (XIC), 44 gene expression from Hox clusters, 45 and imprinted loci revealed that segments of ncRNA bind the PRC2 complex and regulate its activity and localization.…”

Section: Ncrna and Prc2 Recruitmentmentioning

confidence: 99%

Composition, recruitment and regulation of the PRC2 complex

Nayak

Xu²,

Min³

2011

Nucleus

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T he PRC2 histone methyltransferase complex is an important regulator of gene expression programs in metazoans. Gene expression regulation by the PRC2 complex is critical for development and cell differentiation. Several recent studies have begun to shed light on the molecular basis for the physiological function of the PRC2 complex. Here, we discuss some of these results and how they provide new insights into the composition, recruitment and regulation of the PRC2 complex.

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“…Previous reports have shown that lncRNAs have multifunctional roles in modulating embryonic pluripotency, differentiation, development and various diseases, particularly in cancer (11)(12)(13). lncRNAs may be classified according to their mode of action and their functions in cells, including genetic imprinting (14,15), modulating the cancer epigenome (9), serving as molecular decoys (16) and post-transcriptional regulation (10). Accumulating evidence has shown that lncRNA expression profiling may facilitate the diagnosis and prognosis of human cancer, including bladder cancer (17), leukemia (18), breast cancer (9) and rectal cancer (19), suggesting that lncRNAs may serve as effective therapeutic targets for intervention.…”

Section: Introductionmentioning

confidence: 99%

Long non-coding RNA RP5-833A20.1 inhibits proliferation, metastasis and cell cycle progression by suppressing the expression of NFIA in U251 cells

Kang

Nie

et al. 2016

Molecular Medicine Reports

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Abstract. Early reports suggest that nuclear factor IA (NFIA) is important in the pathogenesis of glioma. Our previous study demonstrated that the long non-coding RNA (lncRNA), RP5-833A20.1, suppressed the expression of NFIA in THP-1 macrophage-derived foam cells. However, the effect and possible mechanism of RP5-833A20.1 on glioma remains to be fully elucidated, and whether the NFIA-dependent pathway is involved in its progression has not been investigated. In the present study, the mechanisms by which RP5-833A20.1 regulates the expression of NFIA in glioma were investigated. The expression levels of RP5-833A20.1 and NFIA were determined in U251 cells and clinical samples using reverse transcription-quantitative polymerase chain reaction (PCR) analysis. The effects of RP5-833A20.1 on cell proliferation, invasion, cell cycle and apoptosis were evaluated using in vitro assays. The potential changes in protein expression were investigated using western blot analysis. The methylation status of the CpG island in the NFIA promoter was determined using bisulfite PCR (BSP) sequencing. It was found that the expression of RP5-833A20.1 was downregulated, whereas the expression of NFIA was upregulated in glioma tissues, compared with corresponding adjacent nontumor tissues from 20 patients with glioma. The overexpression of RP5-833A20.1 inhibited proliferation and cell cycle progression, and induced apoptosis in the U251 cells. The mRNA and protein levels of NFIA were markedly inhibited by overexpression of RP5-833A20.1 in the U251 cells. The overexpression of RP5-833A20.1 increased the expression of microRNA-382-5p in the U251 cells. The BSP assay revealed that the overexpression of RP5-833A20.1 enhanced the methylation level of the NFIA promoter. These results demonstrated that RP5-833A20.1 inhibited tumor cell proliferation, induced apoptosis and inhibited cell-cycle progression by suppressing the expression of NFIA in U251 cells. Collectively, these results indicated RP5-833A20.1 as a novel therapeutic target for glioma.

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The X as Model for RNA's Niche in Epigenomic Regulation

Cited by 70 publications

References 125 publications

The RNA Worlds in Context

The RNA Worlds in Context

Composition, recruitment and regulation of the PRC2 complex

Long non-coding RNA RP5-833A20.1 inhibits proliferation, metastasis and cell cycle progression by suppressing the expression of NFIA in U251 cells

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