The Yeast Genes ROX1, IXR1, SKY1 and Their Effect upon Enzymatic Activities Related to Oxidative Stress

Leiro, Ana García; Lombardero, Silvia Rodríguez; Vázquez, Ángel Vizoso; Siso, M.I.G.; Cerdán, M. Esperanza

doi:10.5772/34147

Cited by 1 publication

(2 citation statements)

References 119 publications

(122 reference statements)

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“…Several S. cerevisiae genes that are induced during hypoxia are also related to the oxidative stress response caused by hydrogen peroxide or metals, including ScCUP1 (CUPrum 1), ScCUP2 (CUPrum 1), ScHSP12 (Heat Shock Protein 12), ScFMP46 (Found in Mitochondrial Proteome 46), ScGRE1 (stress Responsive Gene 1), ScALK1, ScMGM1 (Mitochondrial Genome Maintenance 1), or ScSOD1 (SuperOxide Dismutase 1), among others [51,65,74]. In this sense, we confirmed that depletion of KlIxr1 protein in K. lactis also produces an increased sensitivity to the presence of hydrogen peroxide and cadmium metal, as occurred in S. cerevisiae [23,75]. Furthermore, we show for the first time that KlIxr1 is implied in the transcriptional regulation of the KlYCF1 gene, involved in cadmium detoxification, and binds to its promoter.…”

Section: Discussionsupporting

confidence: 77%

“…Furthermore, KlIXR1 gene knockout generates the opposite phenotype in K. lactis, conferring a sharp sensitivity to cisplatin treatment. Some transcriptional regulators that were previously related to cisplatin resistance or ribosomal biosynthesis in S. cerevisiae [73][74][75] are also differently regulated by ScIxr1 and KlIxr1 in both species, respectively. We have observed these differences comparing mRNA changes of KlABF1, KlUME6, or KlDAL81 after KlIxr1 depletion with those previously reported in S. cerevisiae after ScIXr1 depletion [29].…”

Section: Discussionmentioning

confidence: 95%

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The HMGB Protein KlIxr1, a DNA Binding Regulator of Kluyveromyces lactis Gene Expression Involved in Oxidative Metabolism, Growth, and dNTP Synthesis

et al. 2021

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In the traditional fermentative model yeast Saccharomyces cerevisiae, ScIxr1 is an HMGB (High Mobility Group box B) protein that has been considered as an important regulator of gene transcription in response to external changes like oxygen, carbon source, or nutrient availability. Kluyveromyces lactis is also a useful eukaryotic model, more similar to many human cells due to its respiratory metabolism. We cloned and functionally characterized by different methodologies KlIXR1, which encodes a protein with only 34.4% amino acid sequence similarity to ScIxr1. Our data indicate that both proteins share common functions, including their involvement in the response to hypoxia or oxidative stress induced by hydrogen peroxide or metal treatments, as well as in the control of key regulators for maintenance of the dNTP (deoxyribonucleotide triphosphate) pool and ribosome synthesis. KlIxr1 is able to bind specific regulatory DNA sequences in the promoter of its target genes, which are well conserved between S. cerevisiae and K. lactis. Oppositely, we found important differences between ScIrx1 and KlIxr1 affecting cellular responses to cisplatin or cycloheximide in these yeasts, which could be dependent on specific and non-conserved domains present in these two proteins.

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