The yeast Hot1 transcription factor is critical for activating a single target gene,<i>STL1</i>

Bai, Chen; Tesker, Masha; Engelberg, David

doi:10.1091/mbc.e14-12-1626

Cited by 13 publications

(29 citation statements)

References 67 publications

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“…Once Hog1 bound to the promoter of stl1 , this transcription factor increases the rate of transcription by recruiting the chromatin‐remodeling protein Rpd3 and associating with RNA PolIII and components of the mediator complex (Alepuz et al ., ; De Nadal et al ., ). Interestingly, Hot1 transcription factor seems to bind specifically the promoter of stl1 and is critical for its transcription (Bai et al ., ). Glycerol is also produced in S. cerevisiae through glycolysis and the reduction of the glycolytic intermediate dihydroxyacetone phosphate to glycerol‐3‐phosphate and the subsequent oxidation of NADH to NAD + .…”

Section: Introductionmentioning

confidence: 97%

Glycerol stress in Saccharomyces cerevisiae: Cellular responses and evolved adaptations

Mattenberger

Sabater-Muñoz

Hallsworth

et al. 2017

Environmental Microbiology

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Glycerol synthesis is key to central metabolism and stress biology in Saccharomyces cerevisiae, yet the cellular adjustments needed to respond and adapt to glycerol stress are little understood. Here, we determined impacts of acute and chronic exposures to glycerol stress in S. cerevisiae. Glycerol stress can result from an increase of glycerol concentration in the medium due to the S. cerevisiae fermenting activity or other metabolic activities. Acute glycerol-stress led to a 50% decline in growth rate and altered transcription of more than 40% of genes. The increased genetic diversity in S. cerevisiae population, which had evolved in the standard nutrient medium for hundreds of generations, led to an increase in growth rate and altered transcriptome when such population was transferred to stressful media containing a high concentration of glycerol; 0.41 M (0.990 water activity). Evolution of S. cerevisiae populations during a 10-day period in the glycerol-containing medium led to transcriptome changes and readjustments to improve control of glycerol flux across the membrane, regulation of cell cycle, and more robust stress response; and a remarkable increase of growth rate under glycerol stress. Most of the observed regulatory changes arose in duplicated genes. These findings elucidate the physiological mechanisms, which underlie glycerol-stress response, and longer-term adaptations, in S. cerevisiae; they also have implications for enigmatic aspects of the ecology of this otherwise well-characterized yeast.

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Section: Introductionmentioning

confidence: 97%

Glycerol stress in Saccharomyces cerevisiae: Cellular responses and evolved adaptations

Mattenberger

Sabater-Muñoz

Hallsworth

et al. 2017

Environmental Microbiology

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“…First, Hog1 phosphorylates target transcription factors to promote their binding to downstream genes (34). Second, Hog1 phosphorylates and activates target transcription factors, and these active transcription factors recruit Hog1 to the downstream genes (37). Our results indicate that CgHog1 phosphorylates CgRds2 to resist stress through the first mechanism, given that CgHog1-mediated phosphorylation was essential for CgRds2 binding to downstream genes.…”

Section: Discussionmentioning

confidence: 71%

Cg Hog1-Mediated Cg Rds2 Phosphorylation Alters Glycerophospholipid Composition To Coordinate Osmotic Stress in Candida glabrata

Zhang

Zhu

et al. 2019

Appl Environ Microbiol

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Under stress conditions, Hog1 is required for cell survival through transiently phosphorylating downstream targets and reprogramming gene expression. Here, we report that Candida glabrata Hog1 (CgHog1) interacts with and phosphorylates CgRds2, a zinc cluster transcription factor, in response to osmotic stress. Additionally, we found that deletion of CgRDS2 led to decreases in cell growth and cell survival by 23.4% and 39.6%, respectively, at 1.5 M NaCl, compared with levels of the wild-type strain. This is attributed to significant downregulation of the expression levels of glycerophospholipid metabolism genes. As a result, the content of total glycerophospholipid decreased by 30.3%. Membrane integrity also decreased 47.6% in the Cgrds2Δ strain at 1.5 M NaCl. In contrast, overexpression of CgRDS2 increased the cell growth and cell survival by 10.2% and 6.3%, respectively, owing to a significant increase in the total glycerophospholipid content and increased membrane integrity by 27.2% and 12.1%, respectively, at 1.5 M NaCl, compared with levels for the wild-type strain. However, a strain in which the CgRDS2 gene encodes the replacement of Ser64 and Thr97 residues with alanines (Cgrds22A), harboring a CgRds2 protein that was not phosphorylated by CgHog1, failed to promote glycerophospholipid metabolism and membrane integrity at 1.5 M NaCl. Thus, the above results demonstrate that CgHog1-mediated CgRds2 phosphorylation enhanced glycerophospholipid composition and membrane integrity to resist osmotic stress in C. glabrata. IMPORTANCE This study explored the role of CgHog1-mediated CgRds2 phosphorylation in response to osmotic stress in Candida glabrata. CgHog1 interacts with and phosphorylates CgRds2, a zinc cluster transcription factor, under osmotic stress. Phosphorylated CgRds2 plays an important role in increasing glycerophospholipid composition and membrane integrity, thereby enhancing cell growth and survival.

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“…To investigate the influence of the chromatin context on the dynamics of activation of pSTL1, we moved a region containing the promoter of STL1 and the yECITRINE fluorescent reporter to a distinct, centromeric chromatin domain (figure 4a). The displaced DNA region included 1kb upstream of the STL1 locus, enough to have a fully functional STL1 promoter [33]. To compare pSTL1 activity between its endogenous position and the centromeric one, we first submitted both strains to a 2h hyperosmotic stress and used flow cytometry to quantify the fluorescence at several time points.…”

Section: Resultsmentioning

confidence: 99%

“…To compare pSTL1 activity between its endogenous position and the centromeric one, we first submitted both strains to a 2h hyperosmotic stress and used flow cytometry to quantify the fluorescence at several time points. The activity of centromeric pSTL1 was significantly lower than in wild type cells in two independent clones (figure 4b and supplementary figure 3), although the integrity of the promoter was preserved [33].…”

Section: Resultsmentioning

confidence: 99%

The memory of hyperosmotic stress response in yeast is modulated by gene-positioning

Khalil

Hersen

et al. 2019

Preprint

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words/175) 18Cellular memory is a critical ability displayed by microorganisms in order to adapt to potentially detrimental 19 environmental fluctuations. In the unicellular eukaryote S. cerevisiae cellular memory can take the form of a 20 faster or a decreased response following repeated stresses in cell population. Using microfluidics and 21 fluorescence time-lapse microscopy, we studied how yeasts respond to short-pulsed hyperosmotic stresses at 22 the single-cell level by analyzing the dynamical behavior of the stress responsive STL1 promoter fused to a 23 fluorescent reporter. We established that pSTL1 shows variability in its successive activations following two 24 repeated short stresses. Despite this variability, most cells displayed a memory of past stresses through a 25 decreased activity of pSTL1 upon repeated stress. Notably, we showed that genomic location is important for 26 the memory effect since promoter displacement to a pericentromeric chromatin domain leads to a decreased 27 transcriptional strength of pSTL1 and to the loss of memory. This study provides a quantitative description of a 28 cellular memory that includes single-cell variability and points towards the contribution of the chromatin 29 structure in stress memory. 30 31

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The yeast Hot1 transcription factor is critical for activating a single target gene,STL1

Cited by 13 publications

References 67 publications

Glycerol stress in Saccharomyces cerevisiae: Cellular responses and evolved adaptations

Glycerol stress in Saccharomyces cerevisiae: Cellular responses and evolved adaptations

Cg Hog1-Mediated Cg Rds2 Phosphorylation Alters Glycerophospholipid Composition To Coordinate Osmotic Stress in Candida glabrata

The memory of hyperosmotic stress response in yeast is modulated by gene-positioning

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