The yesN gene encodes a carbohydrate utilization regulatory protein in Lactobacillus plantarum

Xu, Wensheng; Zhang, Yanyan; Manqing, Huang; Yi, Xiao Su; Gao, Xueying; Zhang, Dage; Qi-jun, AI

doi:10.1007/s13213-014-0842-y

Cited by 4 publications

(4 citation statements)

References 14 publications

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“…The yesM-yesN two-component system, which is involved in carbohydrate utilization for bacteria [ 68 ], was enriched from the rhizosphere to rhizoplane microbiome. Several transporter-encoding genes whose products are responsible for importing plant-derived polysaccharide sources to bacterial cells including togBMNA (pectin-associated), fucP (fucose-associated), dgoT ( d -galactonate-associated), araJ (arabinose-associated), nagE ( N -acetylglucosamine-associated), lacE (lactose-associated), and dctS (C4-dicarboxylate sensor kinase) were enriched in the rhizoplane microbiome.…”

Section: Resultsmentioning

confidence: 99%

Huanglongbing impairs the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome

et al. 2017

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BackgroundRoots are the primary site for plant-microbe interactions. Among the three root-associated layers (i.e., rhizosphere, rhizoplane, and endorhiza), the rhizoplane is a key component serving a critical gating role that controls microbial entry into plant roots. The microbial communities colonizing the three layers are believed to be gradually enriched from the bulk soil inoculum. However, it is unknown how this enrichment process, particularly the rhizosphere to rhizoplane step, is affected by biotic stresses, such as disease. In this study, we address this question using the citrus root-associated microbiome as a model.ResultsWe identified the rhizosphere-to-rhizoplane-enriched taxonomic and functional properties of the citrus root-associated microbiome and determined how they were affected by Huanglongbing (HLB), a severe systemic disease caused by Candidatus Liberibacter asiaticus, using metagenomic and metatranscriptomic approaches. Multiple rhizoplane-enriched genera were identified, with Bradyrhizobium and Burkholderia being the most dominant. Plant-derived carbon sources are an important driving force for the enrichment process. The enrichment of functional attributes, such as motility, chemotaxis, secretion systems, and lipopolysaccharide (LPS) synthesis, demonstrated more active microbe-plant interactions on the rhizoplane than the rhizosphere. We observed that HLB impaired the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome in three ways: (1) by decreasing the relative abundance of most rhizoplane-enriched genera; (2) by reducing the relative abundance and/or expression activity of the functional attributes involved in microbe-plant interactions; and (3) by recruiting more functional features involved in autotrophic life cycle adaptation, such as carbon fixation and nitrogen nitrification in the HLB rhizoplane microbiome. Finally, our data showed that inoculation of Burkholderia strains isolated from the healthy citrus root-associated microbiome could trigger the expression of genes involved in induced systemic resistance in inoculated plants.ConclusionsHLB causes decreased relative abundance and/or expression activity of rhizoplane-enriched taxonomic and functional properties, collectively resulting in impaired plant host-microbiome interactions. Manipulation of the citrus root-associated microbiome, for instance, by inoculating citrus roots with beneficial Burkholderia strains, has potential to promote plant health. Our results provide novel insights for understanding the contributions of the community enrichment process of the root-associated microbiome to the plant hosts.Electronic supplementary materialThe online version of this article (doi:10.1186/s40168-017-0304-4) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Huanglongbing impairs the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome

et al. 2017

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“…Additionally, the microorganisms would utilize the yesNM system to regulate the ATP-binding cassette (ABC) transport protein, particularly xylGH, which contributed to sense, uptake, and utilize carbohydrates (i.e., glucose and sucrose). 43 In the R2 reactor, the relative abundances of these two genes were increased by 3.3 and 12.2 folds, respectively. Notably, these microbial adaptation mechanisms enabled microorganisms to sustain high metabolic activity in the presence of ample carbohydrate substrates, contributing to the efficient functioning of anaerobic fermentation processes.…”

Section: ■ Results and Discussionmentioning

confidence: 96%

“…Notably, the genes cssS and cssR , which affiliated to TCSs and could effectively assist to cope with the damage of cellular membrane proteins and prevent harmful accumulation of misfolded proteins, were increased by 158.2 and 285.1% in R2, respectively. Additionally, the microorganisms would utilize the yesNM system to regulate the ATP-binding cassette (ABC) transport protein, particularly xylGH , which contributed to sense, uptake, and utilize carbohydrates (i.e., glucose and sucrose) . In the R2 reactor, the relative abundances of these two genes were increased by 3.3 and 12.2 folds, respectively.…”

Section: Resultsmentioning

confidence: 99%

Tannic Acid Modulation of Substrate Utilization, Microbial Community, and Metabolic Traits in Sludge Anaerobic Fermentation for Volatile Fatty Acid Promotion

Huang,

Wang,

Xia

et al. 2024

Environ. Sci. Technol.

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Anaerobic fermentation is a crucial route to realize effective waste activated sludge (WAS) resource recovery and utilization, while the overall efficiency is commonly restrained by undesirable disruptors (i.e., chemical dewatering agents). This work unveiled the unexpectedly positive effects of biodewatering tannic acid (TA) on the volatile fatty acids (VFAs) biosynthesis during WAS anaerobic fermentation. The total VFAs yield was remarkably increased by 15.6 folds with enriched acetate and butyrate in TA-occurred systems. TA was capable to disintegrate extracellular polymeric substances to promote the overall organics release. However, TA further modulated the soluble proteins structure by hydrogen bonding and hydrophobic interactions, resulting in the decrease of proteins bioavailability and consequential alteration of metabolic substrate feature. These changes reshaped the microbial community and stimulated adaptive regulatory systems in hydrolytic–acidogenic bacteria. The keystone species for carbohydrate metabolism (i.e., Solobacterium and Erysipelotrichaceae) were preferentially enriched. Also, the typical quorum sensing (i.e., enhancing substrate transport) and two-component systems (i.e., sustaining high metabolic activity) were activated to promote the microbial networks connectivity and ecological cooperative behaviors in response to TA stress. Additionally, the metabolic functions responsible for carbohydrate hydrolysis, transmembrane transport, and intracellular metabolism as well as VFA biosynthesis showed increased relative abundance, which maintained high microbial activities for VFAs biosynthesis. This study underscored the advantages of biodewatering TA for WAS treatment in the context of resource recovery and deciphered the interactive mechanisms.

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“…YesN proteins belong to the AraC/XylS family of transcriptional regulators that control the expression of genes with diverse biological functions. 75 The apparent redundancy of regulatory genes may indicate a flexible mechanism associated with nitrogen fixation and assimilation. 76 …”

Section: Resultsmentioning

confidence: 99%

Genome features of a novel hydrocarbonoclastic Chryseobacterium oranimense strain and its comparison to bacterial oil-degraders and to other C. oranimense strains

Ramdass,

Rampersad

2023

DNA Research

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For the first time, we report the whole genome sequence of a hydrocarbonoclastic Chryseobacterium oranimense strain isolated from Trinidad and Tobago (COTT) and its genes involved in the biotransformation of hydrocarbons and xenobiotics through functional annotation. The assembly consisted of 11 contigs with 2,794 predicted protein-coding genes which included a diverse group of gene families involved in aliphatic and polycyclic hydrocarbon degradation. Comparative genomic analyses with 18 crude-oil degrading bacteria in addition to two C. oranimense strains not associated with oil were carried out. The data revealed important differences in terms of annotated genes involved in the hydrocarbon degradation process that may explain the molecular mechanisms of hydrocarbon and xenobiotic biotransformation. Notably, many gene families were expanded to explain COTT’s competitive ability to manage habitat-specific stressors. Gene-based evidence of the metabolic potential of COTT support the application of indigenous microbes for remediation of polluted terrestrial environments and provides a genomic resource for improving our understanding of how to optimize these characteristics for more effective bioremediation.

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The yesN gene encodes a carbohydrate utilization regulatory protein in Lactobacillus plantarum

Cited by 4 publications

References 14 publications

Huanglongbing impairs the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome

Huanglongbing impairs the rhizosphere-to-rhizoplane enrichment process of the citrus root-associated microbiome

Tannic Acid Modulation of Substrate Utilization, Microbial Community, and Metabolic Traits in Sludge Anaerobic Fermentation for Volatile Fatty Acid Promotion

Genome features of a novel hydrocarbonoclastic Chryseobacterium oranimense strain and its comparison to bacterial oil-degraders and to other C. oranimense strains

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