Objective: The present study aimed to assess the effects of Greenical VAM TM , a commercial mycorrhizal fungi tablet comprised of Rhizophagus fasciculatus and Rhizophagus intraradices, on sugarcane growth and yield differences. Methods: Two sugarcane cultivars, Co 0238 and Co 86002, were exposed to two AMF inoculation levels, each replicated three times. Parameters included root colonization, soil properties, nutrient uptake, and sugarcane productivity. Findings: AMF significantly improved root colonization, soil properties, nutrient uptake, and overall sugarcane productivity in both cultivars. Co 0238 performed better, highlighting varietal differences. Our study emphasizes the potential of AMF like Greenical VAM TM to enhance sugarcane development and yield under favorable conditions. AMF root colonization varied between 56% and 76% throughout the studied cultivars, with Co 0238 outperforming Co 86002 in terms of yield parameters. This improvement resulted in improved yield characteristics and overall sugarcane production. Novelty: This research pioneers the use of Greenical VAM TM in sugarcane cultivation, with practical implications for higher yields. Each tablet boasts an impressive spore concentration of 50,000 spores per gram, surpassing existing formulations.