The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages

Malo, Meret Cepero; Duchemin, Anne-Laure; Guglielmi, Luca; Patzel, Eva; Sel, Saadettin; Auffarth, Gerd U.; Carl, Michael; Poggi, Lucia

doi:10.1371/journal.pone.0170356

Cited by 9 publications

(3 citation statements)

References 45 publications

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“…However, the combination of transgenes, genetic mutants and in vivo time-lapse imaging to visualize dynamic processes under physiological conditions would almost certainly help understanding more about the pathophysiology of LESCs/Wif1/Wnt related diseases. For instance, inducible systems to transiently activate Wif1 in injured wild type or Wif1 mutant embryos, which carry additional transgenes like anillin and signaling reporter to simultaneously highlight cell proliferation (Paolini et al, 2015; Cepero Malo et al, 2017) and Wnt signaling activity (Moro et al, 2012) would be an excellent tool to study Wif1 dependent regeneration processes in vivo . The fact that cornea structure, development and maintenance appear comparable between zebrafish and mammals (Pan et al, 2013; Takamiya et al, 2015) should encourage to exploit the zebrafish as in vivo model to understand the role of Wif1 in corneal regeneration and homeostasis.…”

Section: Wif1 In Stem Cell Plasticity and As Therapeutic Target In Thmentioning

confidence: 99%

An Eye on the Wnt Inhibitory Factor Wif1

Poggi

Casarosa

Carl

2018

Front. Cell Dev. Biol.

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The coordinated interplay between extrinsic activating and repressing cell signaling molecules is pivotal for embryonic development and subsequent tissue homeostasis. This is well exemplified by studies on the evolutionarily conserved Wnt signaling pathways. Tight temporal and spatial regulation of Wnt signaling activity is required throughout lifetime, from maternal stages before gastrulation until and throughout adulthood. Outside cells, the action of numerous Wnt ligands is counteracted and fine-tuned by only a handful of well characterized secreted inhibitors, such as for instance Dickkopf, secreted Frizzled Related Proteins and Cerberus. Here, we give an overview of our current understanding of another secreted Wnt signaling antagonist, the Wnt inhibitory factor Wif1. Wif1 can directly interact with various Wnt ligands and inhibits their binding to membrane bound receptors. Epigenetic promoter methylation of Wif1, leading to silencing of its transcription and concomitant up-regulation of Wnt signaling, is a common feature during cancer progression. Furthermore, an increasing number of reports describe Wif1 involvement in regulating processes during embryonic development, which so far has not received as much attention. We will summarize our knowledge on Wif1 function and its mode of action with a particular focus on the zebrafish (Danio rerio). In addition, we highlight the potential of Wif1 research to understand and possibly influence mechanisms underlying eye diseases and regeneration.

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Section: Wif1 In Stem Cell Plasticity and As Therapeutic Target In Thmentioning

confidence: 99%

An Eye on the Wnt Inhibitory Factor Wif1

Poggi

Casarosa

Carl

2018

Front. Cell Dev. Biol.

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“…For example, research with breast, ovarian, lung, bladder, and prostate cancer cell lines revealed that dormancy competent cells entered states of dormancy and survival in response to high matrix stiffness, whereas dormancy-incompetent cells rapidly died. Both proliferative and metabolic activity were inhibited, and chemoresistance was increased in these dormant cells ( 125 – 132 , 145 ). Ongoing research is developing ways to induce cancer cells to exit dormancy by using biomaterials.…”

Section: Implications For Disease Severity and Clinical Outcomementioning

confidence: 99%

Pancreatic Ductal Adenocarcinoma Cortical Mechanics and Clinical Implications

et al. 2022

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Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest cancers due to low therapeutic response rates and poor prognoses. Majority of patients present with symptoms post metastatic spread, which contributes to its overall lethality as the 4th leading cause of cancer-related deaths. Therapeutic approaches thus far target only one or two of the cancer specific hallmarks, such as high proliferation rate, apoptotic evasion, or immune evasion. Recent genomic discoveries reveal that genetic heterogeneity, early micrometastases, and an immunosuppressive tumor microenvironment contribute to the inefficacy of current standard treatments and specific molecular-targeted therapies. To effectively combat cancers like PDAC, we need an innovative approach that can simultaneously impact the multiple hallmarks driving cancer progression. Here, we present the mechanical properties generated by the cell’s cortical cytoskeleton, with a spotlight on PDAC, as an ideal therapeutic target that can concurrently attack multiple systems driving cancer. We start with an introduction to cancer cell mechanics and PDAC followed by a compilation of studies connecting the cortical cytoskeleton and mechanical properties to proliferation, metastasis, immune cell interactions, cancer cell stemness, and/or metabolism. We further elaborate on the implications of these findings in disease progression, therapeutic resistance, and clinical relapse. Manipulation of the cancer cell’s mechanical system has already been shown to prevent metastasis in preclinical models, but it has greater potential for target exploration since it is a foundational property of the cell that regulates various oncogenic behaviors.

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“…Despite some differences in expression, distinct identification of RSCs versus RPCs has been challenging [ 43 ]. New markers or transgenic-labelling of CMZ populations have emerged, and recent molecular labelling has shown that RSCs are specifically labelled by rax/rx2 [ 44 ], whereas the F-actin binding protein, anillin , labels late RPC populations in fish species [ 45 ]. A third CMZ subregion contains committed RPCs that express multiple different proneural genes, and these cells will ultimately produce the post-mitotic cells present in the fourth and final zone found closest to the border with the central retina [ 6 , 11 ].…”

Section: Distinct Cell Types Within the Cmz Niche And Their Proliferative And Multilineage Potentialmentioning

confidence: 99%

Retinal Stem Cell ‘Retirement Plans’: Growth, Regulation and Species Adaptations in the Retinal Ciliary Marginal Zone

Miles

Tropepe

2021

IJMS

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The vertebrate retina develops from a specified group of precursor cells that adopt distinct identities and generate lineages of either the neural retina, retinal pigmented epithelium, or ciliary body. In some species, including teleost fish and amphibians, proliferative cells with stem-cell-like properties capable of continuously supplying new retinal cells post-embryonically have been characterized and extensively studied. This region, termed the ciliary or circumferential marginal zone (CMZ), possibly represents a conserved retinal stem cell niche. In this review, we highlight the research characterizing similar CMZ-like regions, or stem-like cells located at the peripheral margin, across multiple different species. We discuss the proliferative parameters, multipotency and growth mechanisms of these cells to understand how they behave in vivo and how different molecular factors and signalling networks converge at the CMZ niche to regulate their activity. The evidence suggests that the mature retina may have a conserved propensity for homeostatic growth and plasticity and that dysfunction in the regulation of CMZ activity may partially account for dystrophic eye growth diseases such as myopia and hyperopia. A better understanding of the properties of CMZ cells will enable important insight into how an endogenous generative tissue compartment can adapt to altered retinal physiology and potentially even restore vision loss caused by retinal degenerative conditions.

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The Zebrafish Anillin-eGFP Reporter Marks Late Dividing Retinal Precursors and Stem Cells Entering Neuronal Lineages

Cited by 9 publications

References 45 publications

An Eye on the Wnt Inhibitory Factor Wif1

An Eye on the Wnt Inhibitory Factor Wif1

Pancreatic Ductal Adenocarcinoma Cortical Mechanics and Clinical Implications

Retinal Stem Cell ‘Retirement Plans’: Growth, Regulation and Species Adaptations in the Retinal Ciliary Marginal Zone

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