The Zebrafish International Resource Center

Freeman, April; Holland, Ron; Hwang-Shum, Jen-Jen; Lains, David; Matthews, Jennifer L.; Murray, Katrina N.; Nasiadka, Andrzej; Quinn, E.W.; Varga, Zoltán; Westerfield, Monte

doi:10.1201/9781315100999-7

Cited by 3 publications

(2 citation statements)

References 1 publication

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“…This fish is also carrying the mitfa b692 mutation [19] which renders the skin transparent. The absolute mutant lacks melanophore, xanthophore, and most of the iridophore cells causing the transparent phenotype [20]. The golden zebrafish exhibited lightening of the pigmented stripes and golden phenotype.…”

Section: Animal Ethics and Animal Used In The Studymentioning

confidence: 99%

Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains

et al. 2020

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The zebrafish has a tetrachromatic vision that is able to distinguish ultraviolet (UV) and visible wavelengths. Recently, zebrafish color preferences have gained much attention because of the easy setup of the instrument and its usefulness to screen behavior-linked stimuli. However, several published papers dealing with zebrafish color preferences have contradicting results that underscore the importance of method standardization in this field. Different laboratories may report different results because of variations in light source, color intensity, and other parameters such as age, gender, container size, and strain of fish. In this study, we aim to standardize the color preference test in zebrafish by measuring light source position, light intensity, gender, age, animal size to space ratio, and animal strain. Our results showed that color preferences for zebrafish are affected by light position, age, strain, and social interaction of the fish, but not affected by fish gender. We validated that ethanol can significantly induce color preference alteration in zebrafish which may be related to anxiety and depression. We also explored the potential use of the optimized method to examine color preference ranking and index differences in various zebrafish strains and species, such as the tiger barb and glass catfish. In conclusion, zebrafish color preference screening is a powerful tool for high-throughput neuropharmacological applications and the standardized protocol established in this study provides a useful reference for the zebrafish research community.

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Section: Animal Ethics and Animal Used In The Studymentioning

confidence: 99%

Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains

et al. 2020

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“…Establishment of repositories of cryopreserved germplasm (e.g., sperm, eggs, embryos, early life stages) has become an indispensable tool for managing valuable genetic resources. For example, the Zebrafish International Resource Center (ZIRC) (Freeman et al, 2019) has shifted to maintaining more than 12,500 genetic lines (http://zfin.org) as frozen sperm samples in addition to actively used live populations (Varga & Tiersch, 2012). However, reliable and standardized cryopreservation capabilities are concentrated in a small number of well‐equipped national stock centers and laboratories, causing an immense problem for preservation of genetic resources at community levels.…”

Section: Introductionmentioning

confidence: 99%

Exploring pathways toward open‐hardware ecosystems to safeguard genetic resources for biomedical research communities using aquatic model species

Liu,

Koch,

Arregui

et al. 2024

J Exp Zool Pt B

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Development of reliable germplasm repositories is critical for preservation of genetic resources of aquatic species, which are widely utilized to support biomedical innovation by providing a foundational source for naturally occurring variation and development of new variants through genetic manipulations. A significant barrier in repository development is the lack of cryopreservation capability and reproducibility across the research community, posing great risks of losing advances developed from billions of dollars of research investment. The emergence of open scientific hardware has fueled a new movement across biomedical research communities. With the increasing accessibility of consumer‐level fabrication technologies, such as three‐dimensional printers, open hardware devices can be custom designed, and design files distributed to community members for enhancing rigor, reproducibility, and standardization. The overall goal of this review is to explore pathways to create open‐hardware ecosystems among the communities using aquatic model resources for biomedical research. To gain feedback and insights from community members, an interactive workshop focusing on open‐hardware applications in germplasm repository development was held at the 2022 Aquatic Models for Human Disease Conference, Woods Hole, Massachusetts. This work integrates conceptual strategies with practical insights derived from workshop interactions using examples of germplasm repository development. These insights can be generalized for establishment of open‐hardware ecosystems for a broad biomedical research community. The specific objectives were to: (1) introduce an open‐hardware ecosystem concept to support biomedical research; (2) explore pathways toward open‐hardware ecosystems through four major areas, and (3) identify opportunities and future directions.

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Cryopreservation of Pooled Sperm Samples

Matthews

Varga

2021

Methods in Molecular Biology

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The Zebrafish International Resource Center

Cited by 3 publications

References 1 publication

Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains

Method Standardization for Conducting Innate Color Preference Studies in Different Zebrafish Strains

Exploring pathways toward open‐hardware ecosystems to safeguard genetic resources for biomedical research communities using aquatic model species

Cryopreservation of Pooled Sperm Samples

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