The zebrafish stress axis: Molecular fallout from the teleost-specific genome duplication event

Alsop, Derek; Vijayan, Mathilakath M.

doi:10.1016/j.ygcen.2008.09.011

Cited by 190 publications

(113 citation statements)

References 39 publications

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“…However, in situations of chronic stress due to high density, adaptation is possible (Tort, 2011). Differences in time and quantity of cortisol release between different fish species, which were administered different stimuli, have been shown (Alsop & Vijayan, 2009). Differences in the basal values of cortisol were observed among pacus in the present study, in comparison to the plasmatic concentration described by Belo et al (2005).…”

Section: Resultssupporting

confidence: 38%

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Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo

Moraes

Soares

et al. 2012

Pesq. agropec. bras.

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-The objective of this work was to evaluate the effect of food supplementation with vitamin C on macrophage and multinucleated giant cell (MGC) activities of pacus at two stocking densities. The experiment was carried out in a 2x2x3 split-plot factorial arrangement with: 0 and 500 mg kg -1 vitamin C; 5 and 20 kg m -3 stocking densities; and evaluation times at 3, 6, and 12 days after the subcutaneous implantation of glass coverslips (DPI). The number of macrophages and MGC, as well as cortisol and glucose plasma levels were determined. The number of macrophages and MGC with two to five nuclei was significantly greater in fish supplemented with vitamin C at 5 kg m -3 stocking density at 3 DPI in comparison to nonsupplemented ones. The macrophage and MGC counts were lower in fish with high-plasma cortisol concentration. Supplementation with 500 mg vitamin C benefits macrophage activity on foreign-body inflammation, and high-cortisol concentration has suppressive effects on this response.Index terms: Piaractus mesopotamicus, ascorbic acid, glucocorticoid, inflammation, Langhans cells, multinucleated giant cells. Vitamina C e cortisol endógeno em resposta inflamatória tipo corpo estranho em pacusResumo -O objetivo deste trabalho foi avaliar o efeito da suplementação com vitamina C no aumento da atividade de macrófagos e células gigantes multinucleadas (CGM), em pacus mantidos em duas densidades de estocagem. O experimento foi realizado em parcelas subdivididas, em arranjo fatorial 2x2x3 com: 0 e 500 mg kg -1 de vitamina C; densidade de estocagem de 5 e 20 kg m -3 ; e tempos de avaliação de 3, 6 e 12 dias após o implante subcutâneo (DPI) de lamínulas de vidro. Foram determinados o número de macrófagos e de CGM, bem como os níveis de cortisol e glicose no plasma. O número de macrófagos e de CGM com 2 a 5 núcleos foi significativamente maior nos peixes suplementados com vitamina C, à densidade 5 kg m -3 , aos 3 DPI, em relação aos não suplementados. Constatou-se menor quantidade de macrófagos e CGM em peixes com baixa concentração plasmática de cortisol. A suplementação com 500 mg de vitamina C por quilograma de ração beneficia a atividade de macrófagos em inflamações do tipo corpo estranho, e a elevada concentração de cortisol circulante tem efeito supressor nesta resposta.Termos para indexação: Piaractus mesopotamicus, ácido ascórbico, glicocorticoide, inflamação, células de Langhans, células gigantes multinucleadas.

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Section: Resultssupporting

confidence: 38%

“…Increases in plasma cortisol concentration are an indicator of stress among teleost fish (Alsop & Vijayan, 2009). In several species of fish, crowding has been reported to be an aquaculture-related chronic stress factor that reduces growth and affects the inflammatory responses (Belo et al, 2005).…”

Section: Resultsmentioning

confidence: 99%

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Belo

Moraes

Soares

et al. 2012

Pesq. agropec. bras.

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“…Due to this transient profile, the functional significance of maternal cortisol during embryogenesis remains unclear (Alsop and Vijayan, 2009), although it was reported to be involved in the epigenetic programming of the corticosteroid stress axis during rainbow trout development (Auperin and Geslin, 2008).…”

Section: Introductionmentioning

confidence: 99%

The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish

et al. 2011

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In zebrafish, ovulated oocytes contain both maternal cortisol and the mRNA for the glucocorticoid receptor (gr), which is spread as granular structures throughout the ooplasm. At 0.2 hpf, this transcript is relocated in the blastodisc area and partitioned among blastomeres. At 6-8 hpf, it is replaced by zygotic transcript. We used morpholinos to block translation of both maternal and zygotic gr transcripts, and a missplicing morpholino to block post-transcriptionally the zygotic transcript alone. Only knockdown of translation produced an increase of apoptosis and subsequent craniofacial and caudal deformities with severe malformations of neural, vascular, and visceral organs in embryos and 5-dpf larvae. Such defects were rescued with trout gr2 mRNA. Microarray analysis revealed that 114 and 37 highly expressed transcripts were up-and down-regulated, respectively, by maternal Gr protein deficiency in 5-hpf embryos. These results indicate that the maternal gr transcript and protein participate in the maternal programming of zebrafish development. Developmental Dynamics 240:874-889,

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“…reduced frequency of visits to the upper water layer and increased erratic movements in zebrafish (Egan et al, 2009). Levels of stress hormones have also been analyzed in zebrafish (Alsop & Vijayan, 2008a) and numerous similarities between zebrafish and human stress responses have been revealed, which strengthen the translational relevance of zebrafish in fear and anxiety research. For example, the sight of a predator elevates cortisol levels in zebrafish (Barcellos et al, 2007).…”

Section: Pharmacological Analysis Of Fear Responses Of Zebrafishmentioning

confidence: 86%

Zebrafish, a Potential Novel Research Tool for the Analysis and Modeling of Anxiety

Gerlai

2011

Different Views of Anxiety Disorders

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The zebrafish stress axis: Molecular fallout from the teleost-specific genome duplication event

Cited by 190 publications

References 39 publications

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

Vitamin C and endogenous cortisol in foreign-body inflammatory response in pacus

The knockdown of maternal glucocorticoid receptor mRNA alters embryo development in zebrafish

Zebrafish, a Potential Novel Research Tool for the Analysis and Modeling of Anxiety

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