The Zinc Finger Repressor, ZBP-89, Binds to the Silencer Element of the Human Vimentin Gene and Complexes with the Transcriptional Activator, Sp1

c-Jun and the dominant-negative mutant, TAM67, induce vimentin gene expression by interacting with the activator Sp1

“…Mutation of this sequence within À261/ þ 72CAT resulted in little reporter gene activity, which did not increase with the inclusion of additional 5 0 -end DNA up to position À1114 (Izmailova et al, 1999a). Other DNA elements include a TATA box, a PEA3 site (Chen et al, 1996), an NF-kB element (Lilienbaum and Paulin, 1993), a proximal silencer (PS), which binds the repressor protein ZBP-89 (Wieczorek et al, 2000), and an antisilencer element (ASE) (Izmailova and Zehner, 1999b).…”

“…Various 5 0 -deletion constructs À757/ þ 72, À353/ þ 72, or À261/ þ 72 and site-mutated constructs (À353/ þ 72mNF-kB, À353/ þ 72mPEA3, À353/ þ 72mSp1) of the human vimentin promoter were fused to the CAT gene as previously described (Wieczorek et al, 2000;Zhang et al, 2003). The pCMV-c-Jun plasmid and the various mutants (TAM67, DBM-3, and LZM-1) were kindly provided by Dr Michael Birrer (NCI/NIH), pCMV-Sp1 by Dr Juanita Merchant (University of Michigan), pPac-b-gal, pPacSp1, and the various deletion constructs of Sp1 by Dr Robert Tjian (UC-Berkeley), and pPac-c-Jun by Dr John Noti (Guthrie Res.…”

Identification and Characterization of a Transcriptional Regulator for the lck Proximal Promoter

“…Mt␤ (identical to BFCOL1, BERF-1) and its human and rat homologues (ht␤, ZBP-89, ZFP148) are reported to bind regulatory regions of various genes, such as the V␤8.1 promoter and the V␣ silencer of the T-cell receptor genes (17), the gastrin promoter (29), the type I collagen promoter (27), the ␤-enolase enhancer (28), the ornithine decarboxylase promoter (30), the p21WAF1 promoter (31), the matrix metalloproteinase-3 promoter (32), the pT␣ enhancer (33), and the silencer element of the vimentin gene (34). Our data showing that mt␤ is ubiquitously expressed at both mRNA and protein levels are consistent with previously published reports and the fact that mt␤ functions in the various promoters and enhancers in various tissues.…”

“…Moreover, the binding site of mt␤ appears to be important for the pT␣ enhancer element (33). In contrast, mt␤ represses transcription from the gastrin gene (29), the ␤-enolase gene (28), the ornithine decarboxylase gene (30), and the vimentin gene (34). It is currently unknown how mt␤/BFCOL1/BERF-1 (ht␤/ZBP-89/ZFP148 in humans) manifests opposite activities on transcription of different genes.…”

“…In addition, the rat and human homologue of the protein, ZBP-89, has been shown to bind to promoter regions of the gastrin gene (29) and the ornithine decarboxylase promoter (30). It has subsequently been reported that the same zinc finger protein also binds to the p21WAF1 gene (31), the matrix metalloproteinase-3 gene (32), the pT␣ gene (33), and the vimentin gene (34).…”