The Zoonotic Risk ofAncylostoma ceylanicumIsolated from Stray Dogs and Cats in Guangzhou, South China

Abstract

Canine and feline hookworm infection is endemic in many countries with zoonotic transmission representing a potentially significant public health concern. However, there is limited data available on the zoonotic transmission of canine and feline hookworms in China. This study was conducted to evaluate the zoonotic risk of Ancylostoma ceylanicum isolated from stray dogs and cats in Guangzhou, south China. Primer pairs CAF/CAR were designed to amplify complete ITS sequences of obtained A. ceylanicum. The results were compared with fourteen ITS reference sequences of human-derived A. ceylanicum registered in GenBank, and phylogenetic trees were established by using NJ and ML methods. The sequence similarity of three dog-derived and five cat-derived A. ceylanicum with fourteen human-derived A. ceylanicum were 96.8%~100% and 97.8%~100%, respectively. Phylogenetic analysis placed A. ceylanicum isolated from dogs and cats in the same group with A. ceylanicum human isolates. Due to the ability of A. ceylanicum to cause a patent infection in humans, the zoonotic risk arising from dog and cat reservoirs to communities in this region should be determined.

Prevalence of intestinal parasites in dogs of kumaon tarai of uttarakhand

“…Moreover, hookworm pathogenesis in dogs is related to their capability of causing anemia, and in puppies the disease caused by large numbers of Ancylostoma caninum are often fatal (Liu, et al, 2014). Giardia arose as the fourth more prevalent intestinal parasite in our study that is currently the most common cause of parasitic disease in domestic dogs and cats (Gates and Nolan, 2014).…”

Tm-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum

“…Adult A. ceylanicum and A. caninum samples were isolated and identified by Liu et al [ 2 , 6 ], preserved in 75% alcohol, and stored in our laboratory. Fecal samples were collected from stray dogs of pet shelters in Shantou, Foshan, and Shaoguan districts of Guangdong province in China, preserved in 2.5% potassium dichromate, and stored at −20°C for use.…”

“… Samples D22, D23, D28, D34, D55, D60, D74, and D79 were isolated and identified by Liu et al [ 2 ]. Samples G23 and G32 were isolated and identified by Liu et al [ 6 ]. …”

“…According to a recent survey, stray dogs are infected mainly by A. ceylanicum and A. caninum in China [ 2 ]. It was reported that A. ceylanicum has become the second most common species of hookworm that can infect humans in Asia [ 3 , 4 ], especially in Southeast Asian countries such as China [ 5 , 6 ], Japan [ 7 ], Malaysia [ 8 ], Laos [ 9 ], Thailand [ 10 ], and India [ 11 ]. Occasionally, A. caninum can reach adulthood in people [ 1 ].…”

Molecular identification of zoonotic hookworm species in dog faeces from Tanzania

“…Zhu & Gasser (1998) used polymerase chain reaction–single-strand conformation polymorphism (PCR–SSCP), and Gasser et al (1996), Traub et al (2004, 2007), e Silva et al (2006), Palmer et al (2007) and Liu et al (2015) used PCR-linked restriction fragment length polymorphism (PCR-RFLP). Ngui et al (2012b) used PCR-high resolution melting (PCR-HRM), and Conlan et al (2012), Mahdy et al (2012), Ngui et al (2012c), Liu et al (2014) and Oliveira-Arbex et al (2017) used PCR and DNA sequencing. The objective of the present study was to identify the hookworm species present in canine faecal samples from Morogoro, Tanzania using PCR-RFLP and species-specific PCR coupled with Sanger sequencing.…”