THE α‐OXIDASE SYSTEM OF YOUNG PEA LEAVES (<i>Pisum sativum</i>) AS GENERATOR OF ELECTRONICALLY EXCITED STATES. EXCITATION IN THE DARK UNDER NATURAL CONDITIONS

Salim-Hanna, Marta; Čampa, Ana; Cilento, Giuseppe

doi:10.1111/j.1751-1097.1987.tb07894.x

Cited by 25 publications

(5 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Addition of long chain fatty acids to preparation4 from peas (Pihum sativum)-which are known to contain broken chloroplasts-results in red emission and Hill activity (Salim-Hanna et al, 1987). The excitation of the chloroplasts appears not to be brought about by excited species derived from the cleavage of the a-peroxylactone, but rather by a CIEEI, process between the a-peroxylactone and exposed chlorophyll (Eq.…”

Section: Peroxidase Systemsmentioning

confidence: 99%

PHOTOCHEMISTRY and PHOTOBIOLOGY WITHOUT LIGHT

Cilento

Adam

1988

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

Abstract-This review covers the literature since 1980 on chemically and enzymatically generated electronically excited species. The emphasis lies on triplet states of carbonyl products that are derived from dioxetanes and dioxetanones as precursors or from suitable enzymatic oxygenations. Singlet oxygen. an important excited state species in biological processes, is not explicitly treated. The utilization of triplet excited carbonyl products to promote photochemical and photobiological transformations by energy transfer are of primordial interest and not the photomechanistic behavior, photophysical properties and inherent photochemical reactions of such excited state species. Thus, the coverage concentrates on photodamage of DNA and RNA., the photochemistry of favins, vitamin D, tryptophan, arachidonic acid, chlorophyll, lipid peroxidation, urocanase activation, excitation of chlorophlasts, and the aerobic oxidation of Schiff bases derived from amino acids and proteins. The potential perspectives of employing authentic dioxetanes and enzymatically generated dioxetane intermediates as effective photon equivalents in photochemotherapy, phototoxicity, photoaffinity labeling and photogenotoxicity are pointed out, in the hope of stimulating more intensive activity in this emerging and novel bioorganic and photobiological field.

show abstract

Section: Peroxidase Systemsmentioning

confidence: 99%

PHOTOCHEMISTRY and PHOTOBIOLOGY WITHOUT LIGHT

Cilento

Adam

1988

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Using 1_14C labelled myristic acid, a correlation between release of radioactivity (as CO 2 ) and emission is observed (Salim-Hanna et al,1987). It may be significant that the excitation of the chloroplasts is observed only with preparations from very young leaves.…”

Section: -Thiouridinementioning

confidence: 93%

“…Important results obtained with this system are summarized in Scheme 2 (Cilento, 1984;Nassi and Cilento, 1985;Duran et al, 1986). When prepared from peas (Pisum sativum) this system contains broken chloroplasts; addition of the fatty acid results in red emission and Hill activity (Salim-Hanna et al, 1987). The excitation of the chloroplasts appears to be brought about by a CIEEL (Schmidt and Schuster, 1980) process between the a-peroxylactone and exposed chlorophylls.…”

Section: Hcoohmentioning

confidence: 99%

Photobiochemistry without Light: Intracellular Generation and Transfer of Electronic Energy

Cilento

1988

Light in Biology and Medicine

Self Cite

View full text Add to dashboard Cite

“…1 represent the differences between the presence and absence of the substrate in the cucumber powderkhlorophyll system. As in the case of the Pisum sativum preparation (Salim-Hanna et al, 1987), oxygen uptake is slow and tends to level off. Throughout the period of the present study, different preparations showed significant variations in the rate of oxygen uptake as reported by Baardseth et al (1987) both in the absence and presence of the fatty acid.…”

Section: Cucumber Preparationmentioning

confidence: 96%

Α‐oxidase Activity in Plants as Promotor of Electronic Energy

Čampa

Salim-Hanna

Cilento

1989

Photochem & Photobiology

Self Cite

View full text Add to dashboard Cite

The a‐oxidase activity of higher plants acting on long chain fatty acids generates the lower aldehyde in the ground state; however if chlorophyll or chioroplasts are present the chlorophylls are excited most likely by a chemically initiated electron exchange (CIEEL) luminescence process with the putative a‐peroxylactone intermediate. When the aldehyde is substituted for the acid, the lower aldehyde appears in the triplet state. The chiral discrimination observed in the quenching by D‐ and L‐tryptophan of the chlorophyll sensitized emission indicates that the triplet aldehyde is generated within the enzymatic preparation and transfers energy while still bound to the enzyme. Chlorophylls in chioroplasts are excited by addition of a long chain fatty acid or aldehyde. The mechanism, however, is unknown.

show abstract

THE α‐OXIDASE SYSTEM OF YOUNG PEA LEAVES (Pisum sativum) AS GENERATOR OF ELECTRONICALLY EXCITED STATES. EXCITATION IN THE DARK UNDER NATURAL CONDITIONS

Cited by 25 publications

References 25 publications

PHOTOCHEMISTRY and PHOTOBIOLOGY WITHOUT LIGHT

PHOTOCHEMISTRY and PHOTOBIOLOGY WITHOUT LIGHT

Photobiochemistry without Light: Intracellular Generation and Transfer of Electronic Energy

Α‐oxidase Activity in Plants as Promotor of Electronic Energy

Contact Info

Product

Resources

About