The δ subunit of the chloroplast ATPase is plastid‐encoded in the diatom Odontella sinensis

Abstract

A 5,2 kb P.~tl re~trietion trallment eontainlnll the atpA tt©ne cluster or' the plastic I~nom© of th~ centrl~: diatom Od.nrella ,~/.¢))sts wa~ cloned, $¢qu~acin~ revealed a readin$ frame o1" 561 hp separatin~ the ~cne~ atpF at~d I~,tpA, which is preceded by a putative ribosome bindin8 site, The third nueleotide or the codon for the last aminn acid of atpF is the first nucleotid¢ of the initiation codon of the 561 bp reading I'ram¢, The amino acid sequcnc~ deduced from the nueleotlde ~¢qu~nce of this Ilene (atpD) is ¢olinear with t~ subunits of different l~,F).ATPases and shows an overall ~equcnce homolol~y of up to .~5~ when compared with the sequenexs ofcyanobacteria and C, vanophor~ parmloxo. Tl~e results are (li~usscd in context with the evolution of chloroplasts of tl~e chlorophyll.a + b and -a + c lineages, respeetlvely,

Nucleotide sequence and phylogenetic implication of the ATPase subunits ? and ? encoded in the chloroplast genome of the brown alga Dictyota dichotoma

“…Based on low stringency hybridizations it was assumed that one or more copies of the e gene have been transferred to the atpA gene cluster in chromophytic algae [21,24]. Nucleotide sequence comparisons, however, revealed that the two additional reading frames within the atpA gene cluster of the diatom Odontella sinensis [32] (P.G. Pancic, H. Strotmann and K.V.…”

Structural organization of the chloroplast genome of the chromophytic algaVaucheria bursata

“…Unexpectedly, however, both in the brown alga Dictyota dichotoma (M.G. Kuhsel etal., in preparation) and the diatom Odontella sinensis [36], the gene atpD encoding the delta subunit of CF1 turned out to reside in the plastid genome at the same position as in cyanobacteria [7] and the cyanelles of (unpublished results), as has been found in cyanobacteria [ 12]. There is thus no reason to argue for prokaryotes of the Heliobacterium type as the putative ancestors of chromophytic plastids [25].…”

Import and processing of the precursor of the delta subunit of tobacco chloroplast ATP synthase

“…2), although overall levels of sequence conservation are poor. The tobacco /5 subunit contains identical residues at 26-27 ~o of comparable positions of the cyanobacterial 6 subunits [6,15 ], and only 23 ~o identical residues compared to the chloroplast-encoded /5 subunit from Odontella sinensis [ 19].…”

“…Sequences are derived from nucleotide sequences presented as follows: spinach [ 10], Odontella sinensis [ 19], Synechocoecus 6301 [6], Anabaena 7t20 [ 15], Escherichia coli [8 ]. Amino acid residues present in all six sequences are boxed.…”