2011
DOI: 10.1530/joe-11-0194
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The Δ337T mutation on the TRβ causes alterations in growth, adiposity, and hepatic glucose homeostasis in mice

Abstract: Mice bearing the genomic mutation D337T on the thyroid hormone receptor b (TRb) gene present the classical signs of resistance to thyroid hormone (TH), with high serum TH and TSH. This mutant TR is unable to bind TH, remains constitutively bound to co-repressors, and has a dominant negative effect on normal TRs. In this study, we show that homozygous (TRbD337T) mice for this mutation have reduced body weight, length, and body fat content, despite augmented relative food intake and relative increase in serum le… Show more

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Cited by 16 publications
(19 citation statements)
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“…As TRb participates actively in the hormonal feedback regulation, these mice are resistant to TH and exhibit elevated serum T 3 and T 4 . Homozygous mice used in this study were acquired by heterozygous mating pairs, and the genotype was determined by analysis of DNA from the tail employing PCR, using the following primers: 5 0 -ATGGGGAAATGGCAGTGAGAG (match), 5 0 -ATGGG-GAAATGGCAGTGACAC (mismatch) and 3 0 -AGCACACT-CACCTGAAGACAT (out) (Santiago et al 2011). Direct comparisons were made with littermate controls (WT).…”
Section: Animal Care and Treatmentsmentioning
confidence: 99%
See 1 more Smart Citation
“…As TRb participates actively in the hormonal feedback regulation, these mice are resistant to TH and exhibit elevated serum T 3 and T 4 . Homozygous mice used in this study were acquired by heterozygous mating pairs, and the genotype was determined by analysis of DNA from the tail employing PCR, using the following primers: 5 0 -ATGGGGAAATGGCAGTGAGAG (match), 5 0 -ATGGG-GAAATGGCAGTGACAC (mismatch) and 3 0 -AGCACACT-CACCTGAAGACAT (out) (Santiago et al 2011). Direct comparisons were made with littermate controls (WT).…”
Section: Animal Care and Treatmentsmentioning
confidence: 99%
“…Products were amplified on Applied Biosystems 7500 Real-Time PCR System (Life Technologies Co.) using iQ SYBR Green Supermix. Cycle parameters were 50 8C for 2 min and 95 8C for 10 min, followed by 44 cycles at 95 8C for 15 s, 53 8C for 30 s, and 72 8C for 45 s. The intron spanning primers used in this manuscript have been described earlier by our own group and by others (Rodgers et al 2005, Akamine et al 2007, Machado et al 2009, Paula et al 2010, 2012, Santiago et al 2011. Efficiency of each reaction was calculated using a serial dilution and varied from 94% for SIRT1 to 98% for 36B4, and products' purity was confirmed by agarose gel analysis.…”
Section: Animal Care and Treatmentsmentioning
confidence: 99%
“…, was used for one experiment (50). The genotypes of the animals were determined as described previously (50).…”
Section: ⌬337t/⌬337tmentioning
confidence: 99%
“…TRβKI mice, targeted to replicate known human TRβ gene mutations (frame-shift PV and T337D) [64,65], are true models of the dominantly inherited form or RTH. Heterozygous KI mice manifest many of the abnormalities observed in man.…”
Section: Trβ Gene Manipulationsmentioning
confidence: 99%