Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism

Huang, Fengjie; Zheng, Xiaojiao; Ma, Xiaojing; Jiang, Runqiu; Zhou, Wangyi; Zhou, Shuiping; Zhang, Yunjing; Li, Sha; Wang, Shouli; Kuang, Junliang; Xia, Han; Wei, Meilin; You, Yijun; Li, Mengci; Li, Yitao; Liang, Dandan; Liu, Jiajian; Chen, Tianlu; Yan, Chao; Wei, Runmin; Rajani, Cynthia; Shen, Chengxing; Xie, Guoxiang; Bian, Zhaoxiang; Li, Houkai; Zhao, Aihua; Wang, Jia

doi:10.1038/s41467-019-12896-x

Cited by 563 publications

(464 citation statements)

References 45 publications

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“…These results prompt us to anticipate that IBS is a disease with moderate alterations in fecal microbiota and metabolites. Moreover, tea drinking was observed to be associated with fecal microbiota and metabolites in IBS, which is consistent to previous study that tea could have effect on gut microbiota [32]. However, more detail relationship between tea and IBS are needed in further research.…”

Section: Discussionsupporting

confidence: 88%

Integrated Multi-Omics Data Analysis Reveals Altered Metabolome Activity and Microbiome Composition in Irritable Bowel Syndrome

Fang¹,

Zhao²,

Zhou³

et al. 2020

Preprint

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Background: Irritable bowel syndrome (IBS) is one of functional gastrointestinal disorders mainly characterized by chronic and/or recurrent symptoms of abdominal pain and irregular defecation. Changed gut microbiota has been proposed to mediate IBS; however, contradictory results exist, and the exact mechanism is still debatable. IBS-specific microbiota and metabolite remain poorly understand. To address this issue, we performed untargeted metabolomic and shotgun metagenomic profiling of stool and serum samples from discovery (n=330) and validation (n=101) cohorts of IBS and healthy individuals. Results: Fecal “omics” data show moderate dysbiosis compared with other disease, in contrast, serum metabolites show significant differences and have great power to discriminate IBS from healthy subjects. Specifically, 726 differentially abundant serum metabolites are identified, including fatty acyl-CoA enriched in IBS. Integrating microbiome and metabolome data, we identified 522 robust associations between differentially abundant species and fecal metabolites, of which three species are strongly associated with the low abundance of dihydropteroic acid. Moreover, the tryptophan enrichment correlates with the severity of IBS depression in both fecal and serum metabolomes. Conclusions: Collectively, our study unveils serum/fecal metabolome alterations and their relationship with gut microbiome and highlight the massive dysbiosis of serum metabolites which empower to discriminate IBS patients. Our study also provides a valuable resource for future studies to understand host-gut microbiota relationships, and facilitate potential clinical applications using microbiota and (or) metabolites to evaluate IBS patients with depression.

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Section: Discussionsupporting

confidence: 88%

Integrated Multi-Omics Data Analysis Reveals Altered Metabolome Activity and Microbiome Composition in Irritable Bowel Syndrome

Fang¹,

Zhao²,

Zhou³

et al. 2020

Preprint

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“…59 Polyphenols extracted from puerarin (Pueraria lobata), resveratrol, and quercetin can mediate cholesterol efflux and intestinal microbial changes, thereby regulating bile acid excretion and reducing fat production. 60,61 Scutellaria baicalensis can increase the expression of the rate-limiting enzyme cytochrome P450 family 7 subfamily A member 1 (CYP7A1) in the bile acid biosynthesis pathway, and CYP7A1 can prevent the obesity induced by a high-fat diet (the exact mechanism is not clear). 62 Clove, allspice, and aromatic ginger extracts can induce TGR5-mediated BAS weight loss effects.…”

Section: Regulate the Levels Of Bile Acids And Other Metabolitesmentioning

confidence: 99%

<p>The Mechanism of Traditional Chinese Medicine for the Treatment of Obesity</p>

Li¹,

Zhang²,

Li³

2020

DMSO

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Obesity is the lipid deposition caused by the imbalance between energy intake and consumption caused by a variety of factors. Obesity can lead to multiple systemic complications. At present, the treatment of obesity is mainly lifestyle intervention, drug weight loss, and weight loss surgery, but the curative effect is limited or the side effects are serious. Traditional Chinese medicine plays a unique role in the treatment of obesity. Existing studies have found that traditional Chinese medicine can treat obesity in a variety of ways, such as regulating intestinal microflora, enhancing hormone level, regulating fat metabolism, and so on. In this review, we will introduce and summarize the mechanism of traditional Chinese medicine in the treatment of obesity.

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“…Gut bacteria play a profound role in BAs metabolism and are responsible for the modification of BAs via promoting deconjugation, dehydrogenation, and dehydroxylation of primary BAs 9 . Numerous studies have shown that gut microbiota can regulate host physiology by altering BAs metabolism 10‐12 . In addition, the altered BAs metabolism profiles have been considered as a marker of many diseases, such as IBD, Crohn's disease, and Alzheimer's disease 13‐15 …”

Section: Introductionmentioning

confidence: 99%

Hyodeoxycholic acid (HDCA) suppresses intestinal epithelial cell proliferation through FXR‐PI3K/AKT pathway, accompanied by alteration of bile acids metabolism profiles induced by gut bacteria

et al. 2020

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Bile acids (BAs) have been implicated in regulation of intestinal epithelial signaling and function. This study aimed to investigate the effects of hyodeoxycholic acid (HDCA) on intestinal epithelial cell proliferation and explore the underlying mechanisms. IPEC‐J2 cells and weaned piglets were treated with HDCA and the contributions of cellular signaling pathways, BAs metabolism profiles and gut bacteria were assessed. In vitro, HDCA suppressed IPEC‐J2 proliferation via the BAs receptor FXR but not TGR5. In addition, HDCA inhibited the PI3K/AKT pathway, while knockdown of FXR or constitutive activation of AKT eliminated the inhibitory effects of HDCA, suggesting that FXR‐dependent inhibition of PI3K/AKT pathway was involved in HDCA‐suppressed IPEC‐J2 proliferation. In vivo, dietary HDCA inhibited intestinal expression of proliferative markers and PI3K/AKT pathway in weaned piglets. Meanwhile, HDCA altered the BAs metabolism profiles, with decrease in primary BA and increase in total and secondary BAs in feces, and reduction of conjugated BAs in serum. Furthermore, HDCA increased abundance of the gut bacteria associated with BAs metabolism, and thereby induced BAs profiles alternation, which might indirectly contribute to HDCA‐suppressed cell proliferation. Together, HDCA suppressed intestinal epithelial cell proliferation through FXR‐PI3K/AKT signaling pathway, accompanied by alteration of BAs metabolism profiles induced by gut bacteria.

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Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism

Cited by 563 publications

References 45 publications

Integrated Multi-Omics Data Analysis Reveals Altered Metabolome Activity and Microbiome Composition in Irritable Bowel Syndrome

Integrated Multi-Omics Data Analysis Reveals Altered Metabolome Activity and Microbiome Composition in Irritable Bowel Syndrome

<p>The Mechanism of Traditional Chinese Medicine for the Treatment of Obesity</p>

Hyodeoxycholic acid (HDCA) suppresses intestinal epithelial cell proliferation through FXR‐PI3K/AKT pathway, accompanied by alteration of bile acids metabolism profiles induced by gut bacteria

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