Theaflavin-3, 3′-digallate, a black tea polyphenol, attenuates adipocyte-activated inflammatory response of macrophage associated with the switch of M1/M2-like phenotype

Ko, Huey-Jiun; Lo, Chih-Yu; Wang, Be-Jen; Chiou, Robin Y.‐Y.; Lin, Shumei

doi:10.1016/j.jff.2014.09.003

Cited by 33 publications

(17 citation statements)

References 41 publications

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“…In contrast, supplementation of catechin markedly increased the level of p-AMPK, p-SIRT1, p-FOXO3a and p-NF-κB, and gradually closed to the control group. Similar effects were observed for the extracts from grape [6], black tea [22], brown alga [34]. Consistent with our findings, a few studies have also demonstrated that synergistic activation of AMPK and SIRT1 can promote the expression of anti-inflammatory cytokines, and prevent the expression of pro-inflammatory cytokines via NF-κB signaling pathway in LPS-induced cells [35, 36].…”

Section: Discussionsupporting

confidence: 90%

“…Natural products can provide abundant resources for anti-inflammatory compounds. Some dietary polyphenols, such as resveratrol, catechin, and quercetin, have been shown to be effective in preventing or alleviating inflammatory [22, 30]. The current work demonstrated that catechin had a capacity to protect adipocytes from TNF-α deleterious, with the increasing of the cell adipogenesis and viability.…”

Section: Discussionmentioning

confidence: 58%

“…Polyphenols are the main functional compounds of tea and there are a class of flavonoids known as catechins (the most abundant component) which comprise of (–)-epigallocatechin-3-gallate, (–)-epigallocatechin, (–)-epicatechin, and (–)-epicatechin-3-gallate [21]. The previous reports about the anti-inflammatory activity and mechanism of catechin were mainly using lipopolysaccharide (LPS)-induced macrophages [22–24], rarely were found using TNF-α induced 3T3-L1 adipocytes. Mouse 3T3-L1 preadipocytes are commonly recognized as a cell model to study differentiation and metabolism of adipocyte.…”

Section: Introductionmentioning

confidence: 99%

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Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes

et al. 2019

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Chronic inflammation is a fundamental symptom of many diseases. Catechin possesses anti-oxidant and anti-inflammatory properties. However, the mechanism of catechin to prevent inflammation in 3T3-L1 adipocytes caused by TNF-α remains unknown. Therefore, the effects of catechin on the gene expression of cytokines and the activation of cell signals in TNF-α induced 3T3-L1 adipocytes were investigated. The effects of catechin on adipogenesis and cell viability were detected by Oil Red O staining and CCK-8 assay, respectively. The genes expression of cytokines was determined by real-time RT-PCR. The expression of NF-κB, AMPK, FOXO3a and SIRT1 on translation level was determined by western blotting analysis. The results demonstrated that catechin significantly enhanced adipogenesis and cell viability. catechin inhibited the gene expression of pro-inflammatory cytokines including IL-1α, IL-1β, IL-6, IL-12p35, and inflammatory enzymes including iNOS and COX-2, but enhanced the gene expression of anti-inflammatory cytokines including IL-4 and IL-10. Catechin also inhibited the activation of NF-κB, AMPK, FOXO3a and SIRT1, but increased the phosphorylation level of the above factors. All these results indicated that as a potential therapeutic strategy catechin has the ability of attenuating inflammatory response triggered by TNF-α through signaling cascades involved in inflammation and cytokines.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 58%

Section: Introductionmentioning

confidence: 99%

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Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes

et al. 2019

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“…The reason may be that almost all tea plants have white flowers that typically bloom at the end of harvesting season, and these flowers are rarely used due to the low content of healthful compounds. However, some studies showed that total content of catechins and flavonoids in tea flowers were similar to that of leaf, but contained less caffeine [7]. Furthermore, the extract of tea flowers has demonstrated antioxidant properties, due to strong free radical scavenging abilities [8].…”

Section: Introductionmentioning

confidence: 99%

Metabolome and Transcriptome Analysis Reveals Putative Genes Involved in Anthocyanin Accumulation and Coloration in White and Pink Tea (Camellia sinensis) Flower

et al. 2020

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A variant of tea tree (Camellia sinensis (L.)) with purple buds and leaves and pink flowers can be used as a unique ornamental plant. However, the mechanism of flower coloration remains unclear. To elucidate the molecular mechanism of coloration, as well as anthocyanin accumulation in white and pink tea flowers, metabolite profiling and transcriptome sequencing was analyzed in various tea flower developmental stages. Results of metabolomics analysis revealed that three specific anthocyanin substances could be identified, i.e., cyanidin O-syringic acid, petunidin 3-O-glucoside, and pelargonidin 3-O-β-d-glucoside, which only accumulated in pink tea flowers, and were not able to be detected in white flowers. RNA-seq and weighted gene co-expression network analysis revealed eight highly expressed structural genes involved in anthocyanin biosynthetic pathway, and particularly, different expression patterns of flavonol synthase and dihydroflavonol-4-reductase genes were observed. We deduced that the disequilibrium of expression levels in flavonol synthases and dihydroflavonol-4-reductases resulted in different levels of anthocyanin accumulation and coloration in white and pink tea flowers. Results of qRT-PCR performed for 9 key genes suggested that the expression profiles of differentially expressed genes were generally consistent with the results of high-throughput sequencing. These findings provide insight into anthocyanin accumulation and coloration mechanisms during tea flower development, which will contribute to the breeding of pink-flowered and anthocyanin-rich tea cultivars.

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“…More importantly, increased AMPK activity inhibits NFkB activity and also down-regulates pro-inflammatory cytokines such as TNF-α and IL-6. Altogether, changes in the activity of AMPK caused by TF-3 can hinder the NFkB chronic inflammation cascade [53].…”

Section: Discussionmentioning

confidence: 99%

Effects of Methylmercury and Theaflavin Digallate on Adipokines in Mature 3T3-L1 Adipocytes

Chauhan

Dunlap

Duffy

2019

IJMS

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Diabetes is a contributor to morbidity across the globe and is often associated with obesity, metabolic syndrome and other inflammatory diseases associated with aging. In addition to genetic and lifestyle factors, environmental factors such as metals and persistent organic pollutants may increase the severity or lower the threshold of these conditions. In cell culture, methylmercury is toxic to adipocytes and may impact adipokine secretions. In this study, we determined the effects of different concentrations of theaflavin digallate on methylmercury exposed 3T3-L1 adipocytes in cell culture. Secretions of resistin, adiponectin and lipid peroxidation product, 4-hydroxynonenal (4-HNE) were monitored using ELISA assays. Cell morphology of methylmercury and theaflavin-3,3′-digallate treated adipocytes was assessed using Lipid (Oil Red O) staining. Exposure to methylmercury increased the levels of resistin and adiponectin as well as 4-HNE when compared to the control cells. Methylmercury treated cells resulted in smaller number of adipocytes and clumped lipid droplets. These results suggest that methylmercury induces reactive oxygen species leading to development of an inflammatory response. Theaflavin-3,3′-digallate reduced the impact of methylmercury by maintaining the adipocytes morphology and secretion patterns of adiponectin, resistin and 4-hydroxynonenal. With this experimental model system other anti-inflammatory and signaling agents could be tested at the biochemical level before eventually leading to studies in animal models.

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Theaflavin-3, 3′-digallate, a black tea polyphenol, attenuates adipocyte-activated inflammatory response of macrophage associated with the switch of M1/M2-like phenotype

Cited by 33 publications

References 41 publications

Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes

Catechin attenuates TNF-α induced inflammatory response via AMPK-SIRT1 pathway in 3T3-L1 adipocytes

Metabolome and Transcriptome Analysis Reveals Putative Genes Involved in Anthocyanin Accumulation and Coloration in White and Pink Tea (Camellia sinensis) Flower

Effects of Methylmercury and Theaflavin Digallate on Adipokines in Mature 3T3-L1 Adipocytes

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