Theileria annulata: Genetic Characterization of Spanish Isolates by Isoenzyme Electrophoresis and Random Amplified Polymorphic DNA

Sánchez, J.M.; García-Fernández, Pedro

doi:10.1006/expr.1999.4402

Cited by 3 publications

(2 citation statements)

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“…A variety of methods, including mAb (Bishop et al 1993 b;Pinder & Hewett, 2001), isoenzyme analysis (Allsopp, Gibson & Stagg, 1985), oligonucleotide probes to parasite DNA (Conrad et al 1987 ;Allsopp et al 1989 ;Bishop et al 1993 b;Bishop, Spooner & Sohanpal, 1996), arbitrary-primed PCR and RAPDs (Bishop, Sohanpal & Morzaria, 1993 a ;Sparagano, Zanaa & Ambrose, 1998), minisatellites (Bishop, Morzaria & Gobright, 1998), PCR-RFLP (Geysen et al 1999) and sequencing (Toye et al 1995 ;Collins & Allsopp, 1999) have been used to demonstrate genetic variation in Theileria parva. The number of studies genotyping T. annulata is more restricted, but methods used include isoenzyme analysis (Melrose et al 1984 ;Ben Miled et al 1994 ;Martin-Sanchez & Garcia-Fernandez, 1999), mAb (Shiels et al 1986 a ;Ben Miled et al 1994), oligonucleotide or nucleic acid probes (Ben Miled et al 1994 ;Shiels et al 1995), RAPDs (Martin-Sanchez & Garcia-Fernandez, 1999), and sequencing (Katzer et al 1994(Katzer et al , 1998Shiels et al 1995 ;Gubbels et al 2000). The advantage of the PCR-RFLP method used here for analysing DNA from cloned parasites is its speed, allowing a large number of clones to be genotyped in a relatively short time.…”

Section: Discussionmentioning

confidence: 99%

Genetic and phenotypic analysis of Tunisian Theileria annulata clones

et al. 2003

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Many parasite species are known to show high levels of genetic diversity, yet the consequences of this diversity for host-parasite interactions are not well understood. Variation in phenotypic traits such as growth rates and the ability to form transmission stages are raw material for natural and artificial selection to act upon with consequences for the evolution of the parasite species and disease control. In order to study genetic and phenotypic diversity amongst Theileria annulata parasites, a collection of 52 parasite clones was generated from cattle isolates and tick material recently collected in Tunisia. Genetic diversity was assessed using PCR-RFLP and monoclonal antibody markers, and genetically distinct clones selected for further study. Clones varied significantly in their growth rates in culture at 37 degrees C, their viability after a period of culture at 41 degrees C and their differentiation rates into transmission stages after culturing at 41 degrees C. The viability of a clone after culturing at 41 degrees C could not be predicted from its growth rate at 37 degrees C, but across clones, differentiation rates were positively correlated with growth rates at 37 degrees C. All 3 in vitro measures are likely to have relevance to parasite-host interactions in animals with clinical theileriosis, and should be acted on by within-host and between-host selection.

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Section: Discussionmentioning

confidence: 99%

Genetic and phenotypic analysis of Tunisian Theileria annulata clones

et al. 2003

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“…According to the sequences for the Theileria spp. 18S rRNA [4], T. annulata Tams-1 [14], T. orientalis MPSP [16], and T. sinensis MPSP [13] genes, six pairs of primers were synthesized by Shanghai Yingjun Biotechnology Co., Ltd. The primer sequences are listed in Table 1.…”

Section: Primer Design and Synthesismentioning

confidence: 99%

Molecular prevalence of Theileria infections in cattle in Yanbian, north-eastern China

et al. 2020

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Bovine Theileria are tick-borne protozoan parasites that invade bovine erythrocytes and lymphocytes. Three main bovine Theileria species have been identified in China: T. orientalis, T. sinensis, and T. annulata. To examine the prevalence of bovine theileriosis in Yanbian, a total of 584 bovine blood samples were collected from five localities from 2017 to 2019 and analyzed by PCR. Six pairs of oligonucleotide primers directed against the 18S rRNA gene of Theileria spp., Tams-1 gene of T. annulata, MPSP gene of T. orientalis, and T. sinensis, were used to detect these parasites. A sequence analysis of the amplified genes confirmed that the Theileria species were T. orientalis and T. sinensis, without T. annulata. The overall prevalence of Theileria in cattle was 42.81% (250/584). Out of the 584 samples, 159 (27.23%) and 157 (26.88%) were positive for T. sinensis and T. orientalis, respectively, and the mixed infection rate was 11.30% (66/584). The total prevalence of bovine Theileria species in Helong, Hunchun, Longjing, Yanji, and Dunhua was 66.28%, 49.68%, 23.81%, 28.15%, and 0%, respectively. These results provide epidemiological data for the prevention and control of bovine Theileria species in Yanbian, China.

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Türkiye’de Theileria annulata Suşlarının Karakterizasyonu ve Tür İdentifikasyonunda Glukoz Fosfat İzomeraz İzoenzim Elektroforezi

Pekcan¹,

Nalbantoğlu²,

Fidanci³

et al. 2013

Kafkas Univ Vet Fak Derg

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ÖzetTheileria annulata omurgalı konakçılarda kan ve dolaşım sistemini etkileyen ve Hyalomma spp keneleri tarafından nakledilen protozoan parazittir. Suş farklılıkları hastalığın şiddeti üzerine etkili olduğu bilindiğinden tayini önem arz etmektedir. Parazit izoenzim elektroforezi T. annulata yanında diğer bazı protozoa'ların tür ve suş tespitinde de kullanılmaktadır. Parazit enzimlerinin elektroforezi; tür tespiti, attenue canlı aşılarda kullanılmak üzere suş seçimi ve konakçı-parazit etkileşimi ile ilgili önemli bilgiler sağlamaktadır. Bu çalışmada T. annulata suşları arası farklılık nişasta jel glikoz fosfat izomeraz izoenzim elektroforezi tekniği aracılığı ile ortaya konmaya çalışılmıştır. Çalışma sonucunda Türkiye'de farklı bölgelerden elde edilen suşlarda farklılık olduğu ve ortaya konan farklılıkların çalışmaların daha da genişletilerek aşı geliştirme ve benzeri proflaktik uygulamalarda yararlanılmasında faydalı olabileceği kanaatine varılmıştır. Anahtar sözcükler: Theileria annulata, Glikoz fosfat izomeraz Characterization of Theileria annulata Strains in Turkey and Usage of Glucose Phosphate Isoenzyme Electrophoresis in Strain Identification SummaryTheileria annulata is a protozoan parasite of which is transmitted via Hyalomma spp ticks is affecting the blood and circulatory system. It is essential to determine the strain infecting the animal as the severity of the disease closely related with it. Parasite isoenzyme electrophoresis is frequently used to identify type and strain of the many protozoa as in Theileriosis. Parasite isoenzyme determination gives clues in choosing the right strain for attenuated vaccine candidates and parasite-host interaction. In this study, it was aimed to demonstrate and characterize the variety of the serotypes of T. annulata with the biochemical technique called Glucose Phosphate Isomerase (GPI) starch gel electrophoresis. In our study, it is revealed that various serotypes of T. annulata exist in different regions of Turkey and this study can further be expanded to provide substantial data for various prophylactic measures such as vaccine development.

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Theileria annulata: Genetic Characterization of Spanish Isolates by Isoenzyme Electrophoresis and Random Amplified Polymorphic DNA

Cited by 3 publications

References 29 publications

Genetic and phenotypic analysis of Tunisian Theileria annulata clones

Genetic and phenotypic analysis of Tunisian Theileria annulata clones

Molecular prevalence of Theileria infections in cattle in Yanbian, north-eastern China

Türkiye’de Theileria annulata Suşlarının Karakterizasyonu ve Tür İdentifikasyonunda Glukoz Fosfat İzomeraz İzoenzim Elektroforezi

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