Thematic Review Series: Exosomes and Microvesicles: Lipids as Key Components of their Biogenesis and Functions Extracellular vesicles and their content in bioactive lipid mediators: more than a sack of microRNA

Boilard, Éric

doi:10.1194/jlr.r084640

Cited by 138 publications

(90 citation statements)

References 115 publications

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“…In contrast to large microparticles, classical lineage membrane markers like CD11b for granulocytes or CD42a for platelets can't be used (50). However, some previous studies provided information on the origin of EV by their oxylipins content (51,52). (56).…”

Section: Discussionmentioning

confidence: 99%

LTB4 and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis

Surmiak

Gielicz

Stojkov

et al. 2020

Journal of Lipid Research

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Activation of neutrophils is an important mechanism in pathology of granulomatosis with polyangiitis (GPA). In this study, we evaluated if extracellular vesicles (EV) circulating in plasma of GPA patients could contribute to this process. EV from plasma of GPA patients in active stage of the disease (n=10) and healthy controls (n=10) were isolated by ultracentrifugation, characterized by flow cytometry (CD63, CD8) and nanoparticle tracking analysis. Targeted oxylipins lipidomics of EV was performed by HPLC-MS2. EV/oxylipins induced neutrophil extracellular traps (NETs) were analyzed by confocal microscopy and released dsDNA was quantified by PicoGreen fluorescent dye. Reactive oxygen species (ROS) production and neutrophils' EV binding/uptake were evaluated by flow cytometry. Brief priming with granulocyte macrophagescolony stimulating factor (GM-CSF) was required for EV-mediated ROS production and dsDNA release. Priming also increased EV binding/uptake by neutrophils observed, however, only for EV from GPA patients. EV from GPA patients had higher concentrations of leukotriene B 4 (LTB 4) and 5-oxoeicosatetraenoic acid (5-oxo-ETE) as compared to EV from healthy controls. Moreover, neutrophils stimulated with LTB 4 or 5-oxo-ETE produced ROS and released dsDNA in a concentration-dependent manner. These results reveal the potential role of EVs containing oxylipins cargo on ROS production and NET formation by activated neutrophils.

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Section: Discussionmentioning

confidence: 99%

LTB4 and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis

Surmiak

Gielicz

Stojkov

et al. 2020

Journal of Lipid Research

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“…As EVs contain enzymatic machinery for biosynthesis of AA-derived lipid mediators (27), redox lipidomics analysis of oxygenated fatty acids was performed. Higher levels of AA metabolites such as 12-HETE (12-hydroxy-eicosatetraenoic acid), 15-HETE (15-hydroxyeicosatetraenoic acid), and lipoxin A 4 (LXA 4 ) were found in liquidphase EV versus MBV (Fig.…”

Section: Oxygenated Fatty Acids and Phospholipids In Mbv And Liquid-pmentioning

confidence: 99%

Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

et al. 2020

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Biomaterials composed of extracellular matrix (ECM) provide both mechanical support and a reservoir of constructive signaling molecules that promote functional tissue repair. Recently, matrix-bound nanovesicles (MBVs) have been reported as an integral component of ECM bioscaffolds. Although liquid-phase extracellular vesicles (EVs) have been the subject of intense investigation, their similarity to MBV is limited to size and shape. Liquid chromatography–mass spectrometry (LC-MS)–based lipidomics and redox lipidomics were used to conduct a detailed comparison of liquid-phase EV and MBV phospholipids. Combined with comprehensive RNA sequencing and bioinformatic analysis of the intravesicular cargo, we show that MBVs are a distinct and unique subpopulation of EV and a distinguishing feature of ECM-based biomaterials. The results begin to identify the differential biologic activities mediated by EV that are secreted by tissue-resident cells and deposited within the ECM.

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“…The data are summarized in Figure . Arachidonic acid and other polyunsaturated fatty acid signalling molecules have been identified as biomarkers and determined to be involved in regulating distal immune response . In addition, the pro‐coagulant cascade and mediating cartilage regeneration are also important physiological functions of active lipids.…”

Section: Biological Effects Of Active Moleculesmentioning

confidence: 99%

“…determined to be involved in regulating distal immune response. 26,27 In addition, the pro-coagulant cascade and mediating cartilage regeneration are also important physiological functions of active lipids. Phosphatidylserine and tissue factor (TF), which are expressed by MVs, are directly involved in the activation of the coagulation cascade in the body.…”

Section: Ta B L E 1 Bioactive Substances Produced By Mvs From Differementioning

confidence: 99%

The role of microvesicles and its active molecules in regulating cellular biology

Tan

Miao

et al. 2019

J Cellular Molecular Medi

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Cell‐derived microvesicles are membrane vesicles produced by the outward budding of the plasma membrane and released by almost all types of cells. These have been considered as another mechanism of intercellular communication, because they carry active molecules, such as proteins, lipids and nucleic acids. Furthermore, these are present in circulating fluids, such as blood and urine, and are closely correlated to the progression of pathophysiological conditions in many diseases. Recent studies have revealed that microvesicles have a dual effect of damage and protection of receptor cells. However, the nature of the active molecules involved in this effect remains unclear. The present study mainly emphasized the mechanism of microvesicles and the active molecules mediating the different biological effects of receptor cells by affecting autophagy, apoptosis and inflammation pathways. The effective ways of blocking microvesicles and its active molecules in mediating cell damage when microvesicles exert harmful effects were also discussed.

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Thematic Review Series: Exosomes and Microvesicles: Lipids as Key Components of their Biogenesis and Functions Extracellular vesicles and their content in bioactive lipid mediators: more than a sack of microRNA

Cited by 138 publications

References 115 publications

LTB4 and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis

LTB4 and 5-oxo-ETE from extracellular vesicles stimulate neutrophils in granulomatosis with polyangiitis

Lipidomics and RNA sequencing reveal a novel subpopulation of nanovesicle within extracellular matrix biomaterials

The role of microvesicles and its active molecules in regulating cellular biology

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