Theoretical analysis of a site-specific chemiluminescence reaction and its application to quantitation of lipid hydroperoxides

Fukuzawa, Kenji; Yoshimura, Teizo; Fujii, Takeru; Takauchi, Kenkichi; Miki, Masayuki; Tamai, Hiroshi; Mino, Makoto

doi:10.1016/0009-3084(88)90090-4

Cited by 10 publications

(5 citation statements)

References 23 publications

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“…CLA has been reported to be an effective amplifier of the chemiluminescence produced by iron-induced decomposition of lipid hydroperoxides (28). Thus, the produced free radicals (PC-OO • and PC-O • ) can react with CLA, and the resulting oxidized CLA in the excited state (CLA * ox ) emits chemiluminescence at 380 nm to return to its ground state (CLA ox ) (26,27).…”

Section: Resultsmentioning

confidence: 99%

“…In the present study, we applied the chemiluminescence-based HPLC method for the assay of TOO-PC. We used 2-methyl-6-phenyl-3,7-dihydroimidazo[1,2-a]pyrazin-3-one, a Cypridina luciferin analog (CLA), which has been reported to be effective in amplifying the emission by superoxide anion and lipid free radicals (27,28), as a sensitive amplifier of the chemiluminescence produced by iron-induced decomposition of TOO-PC under acidic conditions.…”

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confidence: 99%

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Analysis of the addition products of α‐tocopherol with phosphatidylcholine‐peroxyl radicals by high‐performance liquid chromatography with chemiluminescent detection

Yamauchi

Hara

Murase³

et al. 2000

Lipids

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A chemiluminescence-based high-performance liquid chromatographic method was developed for the analysis of the addition products of alpha-tocopherol with phosphatidylcholine-peroxyl radicals (TOO-PC). The TOO-PC eluted from a reversed-phase column was reacted with a chemiluminescent reagent consisting of a Cypridina luciferin analog and a lipid-soluble iron chelate in acidic methanol at 50 degrees C, and the generated chemiluminescence was monitored. The detection limit for TOO-PC by this method was about 1 pmol. This method was applied to the detection of TOO-PC in the peroxidized membranes prepared from rabbit erythrocyte ghosts. When the erythrocyte ghosts were peroxidized by the addition of a water-soluble free radical initiator, a peak corresponding to TOO-PC was detected on the chromatogram with chemiluminescent detection. The amount of TOO-PC in the erythrocyte membranes increased with the depletion of endogenous alpha-tocopherol. The results indicate that this method proved useful for the detection of the TOO-PC formed by the peroxyl-radical scavenging reactions of alpha-tocopherol in biological systems.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

Analysis of the addition products of α‐tocopherol with phosphatidylcholine‐peroxyl radicals by high‐performance liquid chromatography with chemiluminescent detection

Yamauchi

Hara

Murase³

et al. 2000

Lipids

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“…Previous work has also shown that peroxyl radicals could be produced from dissolved oxygen reacting with alkyl radicals that are generated from chemical "initiators" under the influence of light or temperature (16)(17)(18)(19). Other studies demonstrated that the peroxyl free radical could be generated through the catalytic reaction of ferric and/or ferrous ions on organic peroxide (28)(29)(30)(31). The overall mechanism for oxidation due to these reactive free radicals has been proposed to involve initiation, propagation, and termination steps of the radicals.…”

Section: Mechanism and Kinetics Of Trp Oxidation In Fab Drug Product mentioning

confidence: 99%

Site-Specific Tryptophan Oxidation Induced by Autocatalytic Reaction of Polysorbate 20 in Protein Formulation

Lam¹,

Lai²,

Chan³

et al. 2011

Pharm Res

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“…Determination of alkoxyl radical scavenging activity was performed by measuring the inhibition of the chemiluminescence produced by the reaction of t-butylhydroperoxide with ferrous ions in the presence of luminol as previously described [Fukuzawa et al, 1988;Pascual and Romay, 19921. The reaction mixture consisted of 0.8 ml of 50 Mm glycine buffer, pH 8.6, containing 50 Mm SDS and 0.025 mM luinino].…”

Section: Luminolmentioning

confidence: 99%

Effect of silymarin and silybinin on oxygen radicals

Pascual¹,

Gonz²,

Armesto³

et al. 1993

Drug Development Research

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The aim of this work was to study the scavenging action of the flavonoids, silymarin and silybinin (two well known hepatoprotective agents) against different types of oxygen radicals which were generated by specific chemical reactions and detected by lurninol-sensitized chemilurninescence. A well known scavenger for each radical was used as control for the paradigm and for comparing its effect with those of the flavonoids. It was found that 0.8 pg/ml of silyrnarin and 5.5 mg/ml of silybinin produced the same effect as 3.2 ng/ml superoxide dismutase (SOD). An alkoxy radical scavenging effect similar to the one produced by 0.35 ng/ml of vitamin E was caused by 2 pg/ml silyrnarin and 1.6 nglrnl silybinin. Silybinin was evaluated as a scavenger of hydroxyl radicals by determining the inhibition of the chemiluminescence produced by the Fenton reaction with luminol; this was compared with the results obtained from a more conventional method which determines the inhibition of the damage to 2-deoxyribose by the Fenton reaction. In both methods, inhibition was observed at a relatively high concentration of the flavonoid as compared with ethanol. The action of silyrnarin and silybinin as scavengers of oxygen radicals may explain the protective effect of these flavonoids in certain forms of liver disease where free radicals are involved. However, other properties of these compounds cannot be discarded for their hepatoprotective actions. 0 1993 Wiley-Liss, Inc.

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Theoretical analysis of a site-specific chemiluminescence reaction and its application to quantitation of lipid hydroperoxides

Cited by 10 publications

References 23 publications

Analysis of the addition products of α‐tocopherol with phosphatidylcholine‐peroxyl radicals by high‐performance liquid chromatography with chemiluminescent detection

Analysis of the addition products of α‐tocopherol with phosphatidylcholine‐peroxyl radicals by high‐performance liquid chromatography with chemiluminescent detection

Site-Specific Tryptophan Oxidation Induced by Autocatalytic Reaction of Polysorbate 20 in Protein Formulation

Effect of silymarin and silybinin on oxygen radicals

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