Theoretical and experimental study of enzyme kinetics in a microreactor system with surface-immobilized biocatalyst

Miložič, Nataša; Lubej, Martin; Lakner, Mitja; Žnidaršič–Plazl, Polona; Plazl, Igor

doi:10.1016/j.cej.2016.12.030

Cited by 47 publications

(30 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The droplet‐based automated microreactor systems described in Section 2.3 present a powerful tool for gaining intrinsic reaction knowledge on dissolved enzymes. Besides, microflow reactors facilitate evaluation of reaction kinetic constants, estimation of active biocatalyst concentration, and deactivation parameter also for surface‐immobilized enzymes. Furthermore, evaluation of diffusion coefficients for substrates/products in selected solvents, and in packed‐bed reactors could be efficiently estimated using microflow devices.…”

Section: Microfluidics For High‐throughput Process Parameters Estimationmentioning

confidence: 99%

“…Elimination of high‐throughput process parameters strongly depends on the integration of process analytics in microfluidic toolboxes, such as UV/Vis, infrared (IR), near infrared spectroscopy (NIR), mass spectrometry (MS) or Raman spectroscopy, as well as NMR . Monitoring of substrate/product concentration at the outlet from the microreactor using high‐performance liquid chromatography (HPLC) or HPLC‐MS enabled quenching and dilution, or injection of internal standards using the switching valves. Apart from frequently used fluorescence microscopy, thermal lens microscopy allows for ultra precise detection of single molecules in liquids based on optical absorption measurements, also enabling on‐line model validation of molecular transport in a liquid–liquid microflow .…”

Section: Microfluidics For High‐throughput Process Parameters Estimationmentioning

confidence: 99%

“…Process intensification can be estimated from time‐scale analysis in which the mean residence time, the reaction time, and the diffusion time are compared . Although only a model‐based approach provides accurate quantification of mass transfer limitations in various microreactor configurations, time‐scale analysis could serve as a tool for process description simplification …”

Section: Microflow Processing For Biocatalytic Process Intensificationmentioning

confidence: 99%

“…High surface‐to‐volume ratio of microchannel reactors offers the possibility to obtain relatively high enzyme loads of biocatalysts attached to the surfaces. It also enables attachment of a single layer of cells (Figure a), oriented single‐layer immobilization of enzymes fused with tags (Figure e), and creation of bacterial biofilms (Figure b), which together with short diffusional paths lead to excellent accessibility of a biocatalyst for the substrate and thereby high biocatalyst productivity.…”

Section: Microflow Processing For Biocatalytic Process Intensificationmentioning

confidence: 99%

“…For optimization of reactor and process design, macroscale simulation techniques based on a continuum approximation are typically used . This may not always be adapted to describe transport processes and chemical interactions that occur at the microscale.…”

Section: Biocatalytic Process Integration and Scale‐upmentioning

confidence: 99%

See 4 more Smart Citations

The Promises and the Challenges of Biotransformations in Microflow

Žnidaršič–Plazl

2019

Biotechnology Journal

View full text Add to dashboard Cite

The challenges of transition toward the postpetroleum world shed light on the biocatalysis as the most sustainable way for the valorization of biobased raw materials. However, its industrial exploitation strongly relies on integration with innovative technologies such as microscale processing. Microflow devices remarkably accelerate biocatalyst screening and engineering, as well as evaluation of process parameters, and intensify biocatalytic processes in multiphase systems. The inherent feature of microfluidic devices to operate in a continuous mode brings additional interest for their use in chemoenzymatic cascade systems and in connection with the downstream processing units. Further steps toward automation and analytics integration, as well as computer‐assisted process development, will significantly affect the industrial implementation of biocatalysis and fulfill the promises of the bioeconomy. This review provides an overview of recent examples on implementation of microfluidic devices into various stages of biocatalytic process development comprising ultrahigh‐throughput biocatalyst screening, highly efficient biocatalytic process design including specific immobilization techniques for long‐term biocatalyst use, integration with other (bio)chemical steps, and/or downstream processing.

show abstract

Section: Microfluidics For High‐throughput Process Parameters Estimationmentioning

confidence: 99%

Section: Microfluidics For High‐throughput Process Parameters Estimationmentioning

confidence: 99%

Section: Microflow Processing For Biocatalytic Process Intensificationmentioning

confidence: 99%

Section: Microflow Processing For Biocatalytic Process Intensificationmentioning

confidence: 99%

Section: Biocatalytic Process Integration and Scale‐upmentioning

confidence: 99%

See 3 more Smart Citations

The Promises and the Challenges of Biotransformations in Microflow

Žnidaršič–Plazl

2019

Biotechnology Journal

View full text Add to dashboard Cite

show abstract

General Overview on Immobilization Techniques of Enzymes for Biocatalysis

Romero‐Fernández

Paradisi

2019

Catalyst Immobilization

View full text Add to dashboard Cite

Conscious coupling: The challenges and opportunities of cascading enzymatic microreactors

Gruber

Marques

O’Sullivan

et al. 2017

Biotechnology Journal

View full text Add to dashboard Cite

The continuous production of high value or difficult to synthesize products is of increasing interest to the pharmaceutical industry. Cascading reaction systems have already been employed for chemical synthesis with great success, allowing a quick change in reaction conditions and addition of new reactants as well as removal of side products. A cascading system can remove the need for isolating unstable intermediates, increasing the yield of a synthetic pathway. Based on the success for chemical synthesis, the question arises how cascading systems could be beneficial to chemo‐enzymatic or biocatalytic synthesis. Microreactors, with their rapid mass and heat transfer, small reaction volumes and short diffusion pathways, are promising tools for the development of such processes. In this mini‐review, the authors provide an overview of recent examples of cascaded microreactors. Special attention will be paid to how microreactors are combined and the challenges as well as opportunities that arise from such combinations. Selected chemical reaction cascades will be used to illustrate this concept, before the discussion is widened to include chemo‐enzymatic and multi‐enzyme cascades. The authors also present the state of the art of online and at‐line monitoring for enzymatic microreactor cascades. Finally, the authors review work‐up and purification steps and their integration with microreactor cascades, highlighting the potential and the challenges of integrated cascades.

show abstract

Theoretical and experimental study of enzyme kinetics in a microreactor system with surface-immobilized biocatalyst

Cited by 47 publications

References 21 publications

The Promises and the Challenges of Biotransformations in Microflow

The Promises and the Challenges of Biotransformations in Microflow

General Overview on Immobilization Techniques of Enzymes for Biocatalysis

Conscious coupling: The challenges and opportunities of cascading enzymatic microreactors

Contact Info

Product

Resources

About