Theoretical evaluation of the advantages and limitations of constant pressure versus constant flow rate gradient elution separation in supercritical fluid chromatography

Pauw, Ruben De; Desmet, Gert; Broeckhoven, Ken

doi:10.1016/j.chroma.2013.09.008

Cited by 10 publications

(4 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While constant flow mode is limited by the pressure associated with the maximum viscosity of the mobile phase in the column [25]. Performing gradient separations at constant pressure is attractive as the analysis time is sped up, thereby increasing throughput [26,27]. A disadvantage to constant pressure systems depends on the quantitation aspects of mass-sensitive detectors with a nebulizing interface, such as ESI, where the varying flow rate can contribute to a reduction in nebulizer efficiency [28].…”

Section: Introductionmentioning

confidence: 99%

Development of a 45kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2μm particles

Grinias

Godinho

Franklin

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

Commercial chromatographic instrumentation for bottom-up proteomics is often inadequate to resolve the number of peptides in many samples. This has inspired a number of complex approaches to increase peak capacity, including various multidimensional approaches, and reliance on advancements in mass spectrometry. One-dimensional reversed phase separations are limited by the pressure capabilities of commercial instruments and prevent the realization of greater separation power in terms of speed and resolution inherent to smaller sorbents and ultrahigh pressure liquid chromatography. Many applications with complex samples could benefit from the increased separation performance of long capillary columns packed with sub-2 µm sorbents. Here, we introduce a system that operates at a constant pressure and is capable of separations at pressures up to 45 kpsi. The system consists of a commercially available capillary liquid chromatography instrument, for sample management and gradient creation, and is modified with a storage loop and isolated pneumatic amplifier pump for elevated separation pressure. The system’s performance is assessed with a complex peptide mixture and a range of microcapillary columns packed with sub-2 µm C18 particles.

show abstract

Section: Introductionmentioning

confidence: 99%

Development of a 45kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2μm particles

Grinias

Godinho

Franklin

et al. 2016

Journal of Chromatography A

View full text Add to dashboard Cite

show abstract

“…We can mention that De Pauw et al also theoretically investigated the benefits of constant pressure against constant flow rate gradient elution [104]. So, they developed a model of the density of the fluid during gradient elution.…”

Section: Methods Development and Sfcmentioning

confidence: 99%

Overview of the Analytical Lifecycle of Supercritical Fluid Chromatography Methods

Andri

Dispas

Marini

et al. 2016

AJAC

View full text Add to dashboard Cite

In recent times, the overall interest over Supercritical Fluid Chromatography (SFC) is truly growing within various domains but especially for pharmaceutical analysis. However, in the best of our knowledge modern SFC is not yet applied for drug quality control in the daily routine framework. Among the numerous reported SFC methods, none of them could be found to fully satisfy to all steps of the analytical method lifecycle. Thereby, the present contribution aims to provide an overview of the current and past achievements related to SFC techniques, with a targeted attention to this lifecycle and its successive steps. The included discussions were therefore structured accordingly and emphasizing the analytical method lifecycle in accord with the International Conference on Harmonisation (ICH). Recent and important scientific outputs in the field of analytical SFC, as well as instrumental evolution, qualification strategies, method development methodologies and discussions on the topic of method validation are reviewed.

show abstract

“…The problem can alleviated by applying a concomitant composition and flow rate gradient, where the latter decreases during the run to compensate for the higher viscosity [42]. The flow rate gradient can be programmed such that an almost constant pressure is achieved during the run [43][44][45] or at least that it never exceeds the pressure limitation of the instrument. This requires some trial-and-error as the adsorption of mobile phase components on the stationary phase can affect the pressure profile [42].…”

Section: Effect Of Mobile Phase Composition and Separation Conditions...mentioning

confidence: 99%

Theoretical evaluation of the advantages and limitations of constant pressure versus constant flow rate gradient elution separation in supercritical fluid chromatography

Cited by 10 publications

References 24 publications

Development of a 45kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2μm particles

Development of a 45kpsi ultrahigh pressure liquid chromatography instrument for gradient separations of peptides using long microcapillary columns and sub-2μm particles

Overview of the Analytical Lifecycle of Supercritical Fluid Chromatography Methods

Advances in the limits of separation power in supercritical fluid chromatography

Contact Info

Product

Resources

About