Theoretical insights into the mechanism underlying aflatoxin B1 transformation by the BsCotA-methyl syringate system

Wang, Xiaolu; Cui, Lin; Liu, Mengting; Qi, Zheng; Luo, Huiying; Huang, Huoqing; Tu, Tao; Qin, Xing; Wang, Yuan; Zhang, Jie; Wang, Yaru; Yao, Bin; Bai, Yingguo; Su, Xiaoyun

doi:10.1016/j.ecoenv.2024.116049

Cited by 8 publications

(1 citation statement)

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“…With acetosyringone (AS) as the mediator, Ery4 laccase can completely remove 0.1 μg/mL AFB 1 in vitro after 1 h and likely converts the compounds into AFQ 1 , epi -AFQ 1 , AFB 1 -8,9-dihydrodiol or AFB 1 -dialdehyde, AFB 2a , and AFM 1 ( Loi et al, 2023 ). With methyl syringate (MS) as a mediator, the Bs CotA laccase from Bacillus subtilis oxidizes AFB 1 to AFQ 1 ; in this process, the toxicity of the product is significantly reduced, and the conversion efficiency depends on the dosages of laccase and the mediator ( Wang et al, 2024 ). In another experiment, CotA laccase from Bacillus licheniformis ANSB821 was expressed in E. coli , AFB 1 was directly converted to AFQ 1 and epi -AFQ 1 effectively in the absence of redox mediators ( Figure 2D ), and the degradation rate reached 96% within 12 h at pH 8.0 and 37°C ( Guo et al, 2020 ).…”

Section: Common Mycotoxins and Corresponding Enzymes Used For Detoxif...mentioning

confidence: 99%

Bioenzymatic detoxification of mycotoxins

Liu,

Zhang,

Luan

et al. 2024

Front. Microbiol.

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Mycotoxins are secondary metabolites produced during the growth, storage, and transportation of crops contaminated by fungi and are physiologically toxic to humans and animals. Aflatoxin, zearalenone, deoxynivalenol, ochratoxin, patulin, and fumonisin are the most common mycotoxins and can cause liver and nervous system damage, immune system suppression, and produce carcinogenic effects in humans and animals that have consumed contaminated food. Physical, chemical, and biological methods are generally used to detoxify mycotoxins. Although physical methods, such as heat treatment, irradiation, and adsorption, are fast and simple, they have associated problems including incomplete detoxification, limited applicability, and cause changes in food characteristics (e.g., nutritive value, organoleptic properties, and palatability). Chemical detoxification methods, such as ammonification, ozonation, and peroxidation, pollute the environment and produce food safety risks. In contrast, bioenzymatic methods are advantageous as they achieve selective detoxification and are environmentally friendly and reusable; thus, these methods are the most promising options for the detoxification of mycotoxins. This paper reviews recent research progress on common mycotoxins and the enzymatic principles and mechanisms for their detoxification, analyzes the toxicity of the degradation products and describes the challenges faced by researchers in carrying out enzymatic detoxification. In addition, the application of enzymatic detoxification in food and feed is discussed and future directions for the development of enzymatic detoxification methods are proposed for future in-depth study of enzymatic detoxification methods.

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Section: Common Mycotoxins and Corresponding Enzymes Used For Detoxif...mentioning

confidence: 99%