Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels

Korin, Netanel; Bransky, Avishay; Dinnar, Uri

doi:10.1016/j.jbiomech.2006.10.004

Cited by 53 publications

(50 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although at low shear stresses (less than about 0.05 Pa) single RBCs flip like a coin, they reach a steady orientation for increasing flow strength (13,14) allowed by a tank-tread-like circulation of their membrane (13). Such dropletlike behavior should effectively assist the flow by minimizing disturbances to streamlines and has been successfully used to deduce some mechanical properties of RBC membrane in dilute conditions (15,16). However, steady tank treading occurs only when RBCs are dispersed into a viscous aqueous solution (often of dextran polysaccharides), which is several times more viscous than the inner cytoplasm.…”

mentioning

confidence: 99%

Red cells’ dynamic morphologies govern blood shear thinning under microcirculatory flow conditions

Lanotte

Mauer

Mendez

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

222

221

View full text Add to dashboard Cite

Blood viscosity decreases with shear stress, a property essential for an efficient perfusion of the vascular tree. Shear thinning is intimately related to the dynamics and mutual interactions of RBCs, the major component of blood. Because of the lack of knowledge about the behavior of RBCs under physiological conditions, the link between RBC dynamics and blood rheology remains unsettled. We performed experiments and simulations in microcirculatory flow conditions of viscosity, shear rates, and volume fractions, and our study reveals rich RBC dynamics that govern shear thinning. In contrast to the current paradigm, which assumes that RBCs align steadily around the flow direction while their membranes and cytoplasm circulate, we show that RBCs successively tumble, roll, deform into rolling stomatocytes, and, finally, adopt highly deformed polylobed shapes for increasing shear stresses, even for semidilute volume fractions of the microcirculation. Our results suggest that any pathological change in plasma composition, RBC cytosol viscosity, or membrane mechanical properties will affect the onset of these morphological transitions and should play a central role in pathological blood rheology and flow behavior.

show abstract

mentioning

confidence: 99%

Red cells’ dynamic morphologies govern blood shear thinning under microcirculatory flow conditions

Lanotte

Mauer

Mendez

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

222

221

View full text Add to dashboard Cite

show abstract

“…When suspended in a medium with lower viscosity than cytoplasm, RBC will perform a flipping motion [11]. It not only deforms toward its direction of motion, but also rotates and rolls.…”

Section: Common Motion Form Of Rbcmentioning

confidence: 99%

“…They indicated that microchannels with dimensions on the order of 100 μm still obey macroscale flow theory. Korin et al [11] studied the flow behavior of RBC suspension in a straight microchannel with a 0.05 × 1 mm 2 rectangular cross-section. The influence of shear stress on RBC shape has been studied and good agreement was obtained between experimental measurement and numerical simulation.…”

Section: Introductionmentioning

confidence: 99%

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

2011

View full text Add to dashboard Cite

The size of red blood cells (RBC) is on the same order as the diameter of microvascular vessels. Therefore, blood should be regarded as a two-phase flow system of RBCs suspended in plasma rather than a continuous medium of microcirculation. It is of great physiological and pathological significance to investigate the effects of deformation and aggregation of RBCs on microcirculation. In this study, a visualization experiment was conducted to study the microcirculatory behavior of RBCs in suspension. Motion and deformation of RBCs in a microfluidic chip with straight, divergent, and convergent microchannel sections have been captured by microscope and high-speed camera. Meanwhile, deformation and movement of RBCs were investigated under different viscosity, hematocrit, and flow rate in this system. For low velocity and viscosity, RBCs behaved in their normal biconcave disc shape and their motion was found as a flipping motion: they not only deformed their shapes along the flow direction, but also rolled and rotated themselves. RBCs were also found to aggregate, forming rouleaux at very low flow rate and viscosity. However, for high velocity and viscosity, RBCs deformed obviously under the shear stress. They elongated along the flow direction and performed a tank-treading motion.

show abstract

“…The RBCs have a biconcave shape with a cytoplasm enclosed by a hyperelastic membrane without a nucleus [9]. The diameter of RBCs is approximately 8µm and the thickness of the membrane is 1µm in the centre and 2µm at the edge.…”

Section: Introductionmentioning

confidence: 99%

Progress towards the design and numerical analysis of a 3D microchannel biochip separator

Xue¹,

Marson²,

Patel³

et al. 2011

Numer Methods Biomed Eng

View full text Add to dashboard Cite

This paper reports the design and numerical analysis of a three‐dimensional biochip plasma blood separator using computational fluid dynamics techniques. Based on the initial configuration of a two‐dimensional (2D) separator, five three‐dimensional (3D) microchannel biochip designs are categorically developed through axial and plenary symmetrical expansions. These include the geometric variations of three types of the branch side channels (circular, rectangular, disc) and two types of the main channel (solid and concentric). Ignoring the initial transient behaviour and assuming that steady‐state flow has been established, the behaviour of the blood fluid in the devices is algebraically analysed and numerically modelled. The roles of the relevant microchannel mechanisms, i.e. bifurcation, constriction and bending channel, on promoting the separation process are analysed based on modelling results. The differences among the different 3D implementations are compared and discussed. The advantages of 3D over 2D separator in increasing separation volume and effectively depleting cell‐free layer fluid from the whole cross section circumference are addressed and illustrated. Copyright © 2011 John Wiley & Sons, Ltd.

show abstract

Theoretical model and experimental study of red blood cell (RBC) deformation in microchannels

Cited by 53 publications

References 23 publications

Red cells’ dynamic morphologies govern blood shear thinning under microcirculatory flow conditions

Red cells’ dynamic morphologies govern blood shear thinning under microcirculatory flow conditions

Visualization study of motion and deformation of red blood cells in a microchannel with straight, divergent and convergent sections

Progress towards the design and numerical analysis of a 3D microchannel biochip separator

Contact Info

Product

Resources

About