Theoretical models of separation selectivity for charged compounds in micellar electrokinetic chromatography

Puangpila, Chanida; Petsom, Amorn; Nhujak, Thumnoon

doi:10.1002/elps.201000405

Cited by 3 publications

(2 citation statements)

References 33 publications

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“…By contrast, migration behavior in the MEKC separation of charged analytes under the same conditions additionally depends on the electrophoretic mobility of each analyte; this precludes predicting the sequence of migration times or the effective electrophoretic mobility in MEKC. Puangpila et al constructed theoretical models to predict MEKC separation selectivity based on the relationship between retention selectivity in MEKC and mobility selectivity in CZE. Based on the results, the MEKC separation of charged analytes is improved by a selectivity ratio greater than 1.0 and the opposite holds with a factor smaller than 1.0.…”

Section: Approaches To Improving Resolutionmentioning

confidence: 99%

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Silva

2012

Electrophoresis

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This review article addresses recent methodological and instrumental innovations in MEKC with emphasis on practical aspects. Like its predecessors, this review is intended to provide an updated overview covering work on the most salient methodological contributions to enhancing sensitivity and resolution in MEKC-based determinations published over the past two years. The most widespread approaches to enhancing sensitivity, which include improving "classical" online sample concentration techniques, combinations of on- and off-line sample concentration protocols and recent developments are discussed, and so are modifications of existing MEKC systems with various micellar phases, the use of BGE additives (organic modifiers, chiral selectors, gold nanoparticles) and coated capillaries, and the implementation of 2D separations and chemometric methods to enhance resolution. Instrumental approaches such as MS and LIF are also discussed, and proposals for overcoming the problems typically encountered in directly coupling MEKC with MS, and the recent inception of quantum dots with a great potential for LIF detection in MEKC, are also dealt with. Finally, foreseeable developments on potential future directions are also expressed.

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Section: Approaches To Improving Resolutionmentioning

confidence: 99%

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Silva

2012

Electrophoresis

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“…A new approach was published for explaining and predicting changes in MEKC separation selectively α MEKC of charged compound over a wide range of SDS and k (retention factor) values. This was accomplished by establishing an equation and theoretical models where the separation selectivity was related to the dimensionless values of mobility selectivity in CZE (α CZE ) and retention selectivity (α k ) in MEKC . The authors set up an equation for separation selectivity in MEKC as follow: where α CZE is the mobility selectivity in CZE or the ratio of μ0, for example, μ0,2/μ0,1, α k is the retention selectivity of the SDS surfactant for two analytes or the ratio of k , for example, k 2 / k 1 , and β is the ratio for μ0,1/μmc.…”

Section: Predicting and Explaining Mekc Separation Selectivitymentioning

confidence: 99%

Recent methodological and instrumental development in MEKC

2013

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The review gives an update about the methodological and instrumental developments in micellar electrokinetic capillary chromatography as a type of CE analytical technique. Here, the last two years development of the technique are particularly presented. Recent approaches to improve sensitivity are discussed. Newly introduced concentration techniques and experimental methods for verification of the different mechanisms and processes of micellar electrokinetic chromatography analysis are highlighted. A theoretical model to explain changes in separation and electrophoretic mobility order of fully charged analytes are demonstrated. Modern approaches for improving compatibility of micellar electrokinetic capillary chromatography to mass spectrometry are also reported.

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Micellar Electrokinetic Chromatography

Pyell

2000

Encyclopedia of Analytical Chemistry

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Micellar electrokinetic chromatography (MEKC) is a highly efficient capillary‐format separation technique that is performed with the same experimental setup, which is used with capillary electrophoresis (CE). While the separation principle of CE is based on the differences in the effective electrophoretic mobility of the solutes to be separated (only charged species can be separated), MEKC separation is also based on the differences in the distribution constants between two phases migrating at a different velocity because of electrokinetic effects. In general, the second phase is introduced into the separation electrolyte/background electrolyte (BGE) by adding an ionic surfactant (anionic or cationic) in a concentration above the critical micellar concentration (CMC). Under these conditions, dynamic association colloids (micelles) are formed. These aggregates have an electrophoretic mobility and are able to incorporate solutes or interact strongly with species dissolved in the surrounding medium. MEKC extends the applicability of CE to neutral solutes. In addition, MEKC has been proven to be superior to CE in the separation of very complex mixtures of solutes with similar electrophoretic mobility because of the various parameters that are available for the manipulation of the separation selectivity. MEKC can be regarded as a separation technique with a similar scope to that of reversed‐phase high‐performance liquid chromatography (RPHPLC) and other modes of high‐performance liquid chromatography HPLC competing with these modes regarding the efficiency of the separation system, separation speed, cost, and tolerance to matrix constituents, as in MEKC, no stationary phase is present, which can be fouled by irreversible sorption of matrix constituents of the sample. Owing to the small injection volume (few nanoliters) and the small detection volume, which is necessary to avoid intolerable instrumental band broadening, there is a strong need for online focusing methods and sensitive miniaturized detection techniques.

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Theoretical models of separation selectivity for charged compounds in micellar electrokinetic chromatography

Cited by 3 publications

References 33 publications

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Micellar electrokinetic chromatography: A review of methodological and instrumental innovations focusing on practical aspects

Recent methodological and instrumental development in MEKC

Micellar Electrokinetic Chromatography

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