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Respiratory viruses can be attached to human exhaled particles and spread from person to person through respiratory activities. The purpose of this study is to obtain the quantitative description formula of human exhaled particles in the ventilated room through amount number of numerical simulation calculations and regression statistical analysis of the simulated data. In this study, a combination of numerical simulation and laboratory experiments was used, and the results were tallied preferably. Bacillus subtilis was released as a release source to investigate the migration and distribution of bioaerosol. The results show that under the condition of high air supply velocity, due to the disturbance of human respiration and airflow, the diffusion velocity of exhaled particles was faster and the diffusion range is larger than that of low air supply velocity within the same time frame. No matter where the location of the manikin was in the room, the exhaled particles would spread to the whole room in at least 900 s. The method used in this study could be used to predict the distribution of human exhaled particles concentration in different indoor spaces, such as public transport and hospitals. These findings could provide valuable reference for the location of indoor air purifiers, which plays a guiding role in the construction of a healthy indoor environment.
Respiratory viruses can be attached to human exhaled particles and spread from person to person through respiratory activities. The purpose of this study is to obtain the quantitative description formula of human exhaled particles in the ventilated room through amount number of numerical simulation calculations and regression statistical analysis of the simulated data. In this study, a combination of numerical simulation and laboratory experiments was used, and the results were tallied preferably. Bacillus subtilis was released as a release source to investigate the migration and distribution of bioaerosol. The results show that under the condition of high air supply velocity, due to the disturbance of human respiration and airflow, the diffusion velocity of exhaled particles was faster and the diffusion range is larger than that of low air supply velocity within the same time frame. No matter where the location of the manikin was in the room, the exhaled particles would spread to the whole room in at least 900 s. The method used in this study could be used to predict the distribution of human exhaled particles concentration in different indoor spaces, such as public transport and hospitals. These findings could provide valuable reference for the location of indoor air purifiers, which plays a guiding role in the construction of a healthy indoor environment.
No abstract
As rice is one of the world’s most important food crops, protecting it from fungal diseases is very important for agricultural production. At present, it is difficult to diagnose rice fungal diseases at an early stage using relevant technologies, and there are a lack of rapid detection methods. This study proposes a microfluidic chip-based method combined with microscopic hyperspectral detection of rice fungal disease spores. First, a microfluidic chip with a dual inlet and three-stage structure was designed to separate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores in air. Then, the microscopic hyperspectral instrument was used to collect the hyperspectral data of the fungal disease spores in the enrichment area, and the competitive adaptive reweighting algorithm (CARS) was used to screen the characteristic bands of the spectral data collected from the spores of the two fungal diseases. Finally, the support vector machine (SVM) and convolutional neural network (CNN) were used to build the full-band classification model and the CARS filtered characteristic wavelength classification model, respectively. The results showed that the actual enrichment efficiency of the microfluidic chip designed in this study on Magnaporthe grisea spores and Ustilaginoidea virens spores was 82.67% and 80.70%, respectively. In the established model, the CARS-CNN classification model is the best for the classification of Magnaporthe grisea spores and Ustilaginoidea virens spores, and its F1-core index can reach 0.960 and 0.949, respectively. This study can effectively isolate and enrich Magnaporthe grisea spores and Ustilaginoidea virens spores, providing new methods and ideas for early detection of rice fungal disease spores.
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