Theoretical tissue compartment inert gas pressures during a deep dive with and without deep decompression stops: a case analysis

Buzzacott, Peter; Papadopoulou, Virginie; Baddeley, Adrian; Petri, Nadan M.; Lind, Folke

doi:10.5603/imh.2015.0010

Cited by 2 publications

(1 citation statement)

References 3 publications

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“…Improperly segmented cells including uncontracted melanophores were manually corrected in ImageJ and replaced with circles of diameter 15 pixels. Melanophore nearest neighbor distances were calculated using the spatstat package in R [88].…”

Section: Phenotyping Of Adults and Longitudinal Imaging During Developmentmentioning

confidence: 99%

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

et al. 2021

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Vertebrate pigmentation is a fundamentally important, multifaceted phenotype. Zebrafish, Danio rerio, has been a valuable model for understanding genetics and development of pigment pattern formation due to its genetic and experimental tractability, advantages that are shared across several Danio species having a striking array of pigment patterns. Here, we use the sister species D. quagga and D. kyathit, with stripes and spots, respectively, to understand how natural genetic variation impacts phenotypes at cellular and organismal levels. We first show that D. quagga and D. kyathit phenotypes resemble those of wild-type D. rerio and several single locus mutants of D. rerio, respectively, in a morphospace defined by pattern variation along dorsoventral and anteroposterior axes. We then identify differences in patterning at the cellular level between D. quagga and D. kyathit by repeated daily imaging during pattern development and quantitative comparisons of adult phenotypes, revealing that patterns are similar initially but diverge ontogenetically. To assess the genetic architecture of these differences, we employ reduced-representation sequencing of second-generation hybrids. Despite the similarity of D. quagga to D. rerio, and D. kyathit to some D. rerio mutants, our analyses reveal a complex genetic basis for differences between D. quagga and D. kyathit, with several quantitative trait loci contributing to variation in overall pattern and cellular phenotypes, epistatic interactions between loci, and abundant segregating variation within species. Our findings provide a window into the evolutionary genetics of pattern-forming mechanisms in Danio and highlight the complexity of differences that can arise even between sister species. Further studies of natural genetic diversity underlying pattern variation in D. quagga and D. kyathit should provide insights complementary to those from zebrafish mutant phenotypes and more distant species comparisons.

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Section: Phenotyping Of Adults and Longitudinal Imaging During Developmentmentioning

confidence: 99%

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

et al. 2021

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Could some aviation deep vein thrombosis be a form of decompression sickness?

Buzzacott

Møllerløkken

2016

J Thromb Thrombolysis

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Aviation deep vein thrombosis is a challenge poorly understood in modern aviation. The aim of the present project was to determine if cabin decompression might favor formation of vascular bubbles in commercial air travelers. Thirty commercial flights were taken. Cabin pressure was noted at take-off and at every minute following, until the pressure stabilized. These time-pressure profiles were imported into the statistics program R and analyzed using the package SCUBA. Greatest pressure differentials between tissues and cabin pressures were estimated for 20, 40, 60, 80 and 120 min half-time compartments. Time to decompress ranged from 11 to 47 min. The greatest drop in cabin pressure was from 1022 to 776 mBar, equivalent to a saturated diver ascending from 2.46 msw depth. Mean pressure drop in flights >2 h duration was 193 mBar, while mean pressure drop in flights <2 h was 165 mBar. The greatest drop in pressure over 1 min was 28 mBar. Over 30 commercial flights it was found that the drop in cabin pressure was commensurate with that found to cause bubbles in man. Both the US Navy and the Royal Navy mandate far slower decompression from states of saturation, being 1.7 and 1.9 mBar/min respectively. The median overall rate of decompression found in this study was 8.5 mBar/min, five times the rate prescribed for USN saturation divers. The tissues associated with hypobaric bubble formation are likely slower than those associated with bounce diving, with 60 min a potentially useful index.

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Theoretical tissue compartment inert gas pressures during a deep dive with and without deep decompression stops: a case analysis

Cited by 2 publications

References 3 publications

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

A complex genetic architecture in zebrafish relatives Danio quagga and D. kyathit underlies development of stripes and spots

Could some aviation deep vein thrombosis be a form of decompression sickness?

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