Theoretical treatment of diffusion and kinetics of osmium redox polymer mediated glucose oxidase enzyme electrodes: Analytical expression of current density for varying potential

Kirthiga, M.; Rajendran, L.; Fernandez, Carlos

doi:10.1016/j.electacta.2017.01.149

Cited by 13 publications

(4 citation statements)

References 26 publications

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“…The most used enzyme in fuel cells is glucose oxidase (GOx), which oxidizes glucose into gluconolactone at the anode [23]. A wide variety of materials have been used for the immobilization of this enzyme such as carbon nanotubes [24][25][26], graphene [27], polymers [24,25], metallic nanoparticles [27,28], etc. However, there are two important limitations of GOx immobilized on solid electrodes: poor electrical communication between the GOx active site and electrode surface, and enzyme leaching [29].…”

Section: Introductionmentioning

confidence: 99%

Development of bioanode for versatile applications: microfuel cell system in the presence of alcohol and glucose

Ledesma‐García

Gurrola

Trejo-Arroyo

et al. 2022

Mater Renew Sustain Energy

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The purpose of this work is to develop a bioanode using the enzymes of glucose oxidase (GOx) and alcohol dehydrogenase (ADH) as catalysts to oxidised glucose and alcohol present in different beverages. The study was carried out using the covalent bonding method for both enzymes via the functionalization of carbon nanofibers for the formation of carboxyl groups that can form bonds with the amine groups of the enzyme, as well as using tetrabutylammonium bromide (TBAB) with Nafion. The optimum operation parameters of both enzymes (pH and temperature) were determined for the later evaluation in a microfluidic fuel cell. In addition, using the scanning electrochemical microscopy technique, a local study of enzymatic processes is used to demonstrate that the enzymes immobilized on the same electrode remain active. The evaluation of the microfluidic fuel cell was carried out using different solutions, 0.01 M glucose, 0.01 M ethanol and a mixture of 0.01 M glucose and 0.01 M ethanol, all in phosphate buffer solutions at pH 7, where it was possible to obtain a maximum performance of 5.07 ± 0.1 mW cm−2, and there was a significant increase in current density compared to non-composite solutions (glucose or ethanol). In addition, different alcoholic beverages were used to evaluate the versatility and adaptability of the bi-enzymatic anode electrode with the perspective use in Lab-on-a-Chip systems.

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Section: Introductionmentioning

confidence: 99%

Development of bioanode for versatile applications: microfuel cell system in the presence of alcohol and glucose

Ledesma‐García

Gurrola

Trejo-Arroyo

et al. 2022

Mater Renew Sustain Energy

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“…The first generation uses oxygen as the electron acceptor of the enzyme reaction, and the resulting hydrogen peroxide is measured electrochemically. The second generation uses artificial electron acceptors (mediators) for the enzyme reactions, and the reduced electron acceptors are measured by electrodes [23,24]. The third generation employs enzymes with the ability to transfer electrons directly to the electrode without the need for mediators or oxygen [25].…”

Section: Introductionmentioning

confidence: 99%

Light‐Addressable Amperometric Sensor with Counter and Working Electrodes of the Same Material

Kosugi

Hasegawa

Uchida

2021

IEEJ Transactions Elec Engng

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High‐throughput screening is important in various fields, and electrochemical approaches are effective because of advantages such as fast response time and electrode array. Hence, we developed a two‐dimensional electrochemical array sensor. However, its signal intensities for lower concentrations of the redox pair (100 μM–100 pM [Fe[CN]6]3−/4−) were unstable; Therefore, in this study, we investigated and demonstrated a method for improving the signal stability at lower concentrations. Two types of counter electrodes were prepared to assess their influence; the material of the first electrode was the same as that of the working electrode (same material condition), and the other was prepared from a different material (different material condition). First, the differences in the electric potentials between different materials were investigated at lower concentrations. Furthermore, the concentration dependence was investigated using our newly developed light‐addressable amperometric sensor for the two conditions. In the different condition, it was observed that differences in the electric potentials between the electrodes at lower concentrations. It was also observed that the signal intensity was more stable for the same material condition than the different material condition. Limit of detection(LOD) for the same material condition was lower than that under the different material condition. The proposed method using the same materials is thus an effective means to measure low concentrations of redox ions and expected to contribute to a fabrication of a smaller and more stable device without reference electrode. © 2021 Institute of Electrical Engineers of Japan. Published by Wiley Periodicals LLC.

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“…This research has focused on osmium‐based redox polymers as they exhibit several advantages such as lower ionization energy, which leads to a subsequent stabilization of higher oxidation states, lower redox potentials and greater extension of the metal d orbitals, leading to enhanced metal‐ligand back bonding and providing increased complex stability . From the first applications of these redox polymers for reagentless mediated biosensing , they attract much attention due to their efficient electron shuttling properties combined with the polymeric structure promoting a stable adsorption as well as the possibility for multiple layers of immobilized protein molecules, biological membranes or bacterial cells on the electrode surface . So far they have been used for immobilizing many biological catalysts including sugar oxidizing enzymes GOx, oligosaccharide dehydrogenase, galactose oxidase (GaOx), PyOx, pyranose dehydrogenase, PQQ‐ and FAD‐dependent glucose dehydrogenase, cellobiose dehydrogenase, fructose dehydrogenase etc.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Flow Injection Analysis of Glucose and Galactose Based on Engineered Pyranose 2‐Oxidases and Osmium Polymers for Biosensor Applications

et al. 2018

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In the present study, wild type and three mutants of pyranose 2‐oxidase (PyOx‐WT, PyOx‐MT1, PyOx‐MT2, PyOx‐MT3), which showed improved properties for D‐galactose oxidation, were investigated for their oxidising ability when immobilised on graphite electrodes. Four different flexible Os polymers with formal potentials ranging between −0.140 and 0.270 V vs. Ag|AgCl0.1 M KCl were applied together with the various forms of PyOx to wire graphite electrodes using polyethylene glycol diglycidyl ether as crosslinking reagent. The pH profiles for the electrodes modified with wild type and all PyOx mutants in combination with the Os polymers were investigated with both glucose and galactose, respectively, since the PyOx variants showed an improved catalytic activity for galactose. All modified electrodes showed highest response in the pH range between 8.5–10 and KM, Imax values for both glucose and galactose were determined. To prove the catalytic activity, the biosensors were also characterized with cyclic voltammetry. A protein amount 0.26 U was found optimum for PyOx‐WT, 0.36 U for PyOx‐MT1, 0.41 U for PyOx‐MT2 and 0.28 U for PyOx‐MT3 and the analytical characterization of the enzyme electrodes was performed for glucose and galactose under optimized conditions.

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Theoretical treatment of diffusion and kinetics of osmium redox polymer mediated glucose oxidase enzyme electrodes: Analytical expression of current density for varying potential

Cited by 13 publications

References 26 publications

Development of bioanode for versatile applications: microfuel cell system in the presence of alcohol and glucose

Development of bioanode for versatile applications: microfuel cell system in the presence of alcohol and glucose

Light‐Addressable Amperometric Sensor with Counter and Working Electrodes of the Same Material

Amperometric Flow Injection Analysis of Glucose and Galactose Based on Engineered Pyranose 2‐Oxidases and Osmium Polymers for Biosensor Applications

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