Theory and Practice of Histological Techniques

Drury, R. A. B.

doi:10.1136/jcp.36.5.609-d

Cited by 30 publications

(12 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dehydrated brain tissue was cleaned by xylene and entrenched in paraffin wax. Brain sections of 0.5 μm thickness were prepared and then stained with hematoxylin and eosin dye for photomicroscopic observation ( Drury, 1983 ).…”

Section: Methodsmentioning

confidence: 99%

Comparative Evaluation of Prosopis cineraria (L.) Druce and Its ZnO Nanoparticles on Scopolamine Induced Amnesia

et al. 2018

View full text Add to dashboard Cite

Over recent years, utilization of green synthesized nanomaterials has been widely growing on human body because of its special properties. With the increasing acceptance of nanoparticle approach for various clinical treatments, the biosafety and toxicological effects on the vital organs such as central nervous system, have received more concern. Main focus of this study was to evaluate acute exposure of n-butanol fraction of Prosopis cineraria (L.) Druce hydroethanolic extract (BuPC) and green synthesized zinc oxide nanoparticles of BuPC (ZnOPC) on spatial cognition behavior, and to assess underlying mechanism by estimation of enzymatic antioxidative status along with acetylcholinesterase (AChE) activity in mice brain. Strongest in vitro antioxidant and AChE inhibitory activity exhibiting fraction, BuPC, was examined for inhibition kinetic study by Lineweaver–Burk and Dixon plots. BuPC was further used for fabrication ZnOPC and characterized by UV-visible spectroscopy, Fourier Transform Infrared (FTIR), Field Emission Scanning Electron Microscopy (FESEM), Energy Dispersive X ray (EDX), and Dynamic Light Scattering (DLS) analysis. Old male swiss albino mice were randomly divided into seven groups and treated for 21 days. Subsequently spatial memory was determined by two behavioral models [Elevated plus maze (EPM) and Hebbs William maze (HWM)] and supernatant of brain homogenate was analyzed for enzymatic antioxidant level and AChE inhibitory activity. Zinc content of blood plasma and brain was estimated. Results showed prolonged transfer latency (TL) and time taken to reach reward chamber (TRC) by scopolamine was not ameliorated by the ZnOPC group, whereas BuPC group showed significant reduction in scopolamine induced increase in TL and TRC compared to control and scopolamine treated groups. ZnOPC alleviated enzymatic antioxidant activity and AChE as compared to donepezil and BuPC treated groups. Study concludes that ZnOPC attenuated spatial learning and memory by increase in oxidative stress and decrease in AChE activity at both dose levels. Our results suggest that BuPC exhibited a strong neuroprotective effect on cognitive deficit mice and it may be employed as a strong substance for the treatment of dementia whereas the green synthesized ZnOPC was not proficient to reverse the memory impairment induced by scopolamine.

show abstract

Section: Methodsmentioning

confidence: 99%

Comparative Evaluation of Prosopis cineraria (L.) Druce and Its ZnO Nanoparticles on Scopolamine Induced Amnesia

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Three pieces of 5 mm-thick sections of bone biopsies were submerged in three 250 ml Pyrex Squat Beakers each containing 100 ml of 5% aqueous hydrochloric acid (HCl), 5% aqueous nitric acid (HNO 3 ), and 10% ethylenediaminetetraacetic acid (EDTA) placed at room temperature (average 28°C), see Table 1 . The end point of decalcification was checked using the calcium oxalate method [ 14 ] for the two acid decalcifiers (5% HNO 3 and 5% HCl) after a two-hour interval as follows: 5 ml of the used decalcifying fluid was taken and placed in a test tube, then litmus paper was added, and ammonia hydroxide was added drop by drop until the litmus paper changed, indicating alkaline pH decalcifying fluid was clear. 5 ml saturated ammonium oxalate solution was added when the decalcifying solution became turbid, indicating the presence of calcium within bone tissue so that the decalcifying solution was replaced with new solution, and the process was repeated every 30 minutes until the completion of the decalcification process.…”

Section: Methodsmentioning

confidence: 99%

“…The tissues were embedded in paraffin blocks and were sectioned to a thickness of 5-6 μ m using a rotary microtome. Sections were stained with Mayer's haematoxylin, as described by Mayer in 1903, and the counterstain in each was 1% eosin (HE) [ 14 ]. Gridley's stain was used for fungi demonstration as described by Gridley in 1953); after deparaffinization and rehydration, tissue sections were placed in 2% chromic acid for 30 minutes.…”

Section: Methodsmentioning

confidence: 99%

Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma

Salih

2020

Biochemistry Research International

View full text Add to dashboard Cite

Mycetoma is a lifelong granulomatous disease of subcutaneous tissues and bones. Histopathology is a substantiated indicative method based on the assumption of a definitive diagnosis of mycetoma. It requires efficient processing of tissues including bone decalcification. The decalcification process must ensure complete removal of calcium and also a proper preservation of tissue and microorganisms’ staining ability. Objectives. To compare the conventional method used in decalcification with the microwave method using different decalcification solutions. Different characteristics were tested, including the speed of decalcification and morphological and fungal preservation in bone tissue affected with mycetoma. Materials and Methods. Three decalcification solutions were employed to remove calcium from 50 bone tissue samples affected with mycetoma, including 10% neutral buffered EDTA (pH 7.4), 5% nitric acid, and 5% hydrochloric acid. Conventional and microwave methods were used. Haematoxylin-eosin (HE) stain, Gridley’s stain, and Grocott hexamine-silver stain were employed to evaluate the bone and fungi morphologies. Results. The decalcification time of the conventional method compared with the microwave method with 10% EDTA (pH 7.4) took 120 hours and 29 hours, while 5% hydrochloric acid and 5% nitric acid took 8 hours and 3 hours, separately. Also, 10% EDTA is the best decalcifying agent for HE staining and fungal stains. 5% hydrochloric acid and 5% nitric acid can be used for fungal staining. Conclusion. The current study investigated the effects of different decalcifying agents as well as two decalcification procedures on the preservation of the bone structure and fungal staining, which will help to develop suitable protocols for the analyses of the bone tissue affected with mycetoma infection.

show abstract

“…After 24 hours, tissue was allowed to dehydrate in ethanol, processed with xylene and fixed in paraffin wax. Hippocampus sections (0.5 μm) were cut and dyed with hematoxylin and eosin for photomicroscopic examination 50 …”

Section: Methodsmentioning

confidence: 99%

“…Hippocampus sections (0.5 μm) were cut and dyed with hematoxylin and eosin for photomicroscopic examination. 50…”

Section: Histological Studiesmentioning

confidence: 99%

Biofabricated zinc oxide nanoparticles impair cognitive function via modulating oxidative stress and acetylcholinesterase level in mice

Yadav

2020

Environmental Toxicology

View full text Add to dashboard Cite

Current work was designed to explore the effect of ZnO nanoparticles (ZnONP) biofabricated by using Trianthema portulacastrum (TP) leaves extract on mice brain hippocampus. ZnO nanoparticles of TP leaves (ZnOTP) were synthesized by co‐precipitation method and further characterized by using various techniques such as UV‐Vis spectrophotometer, Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infrared (FTIR), and Energy Dispersive X‐ray (EDX). ZnOTP were evaluated for in vitro antioxidant activity, in vivo behavior models (for assessment of cognitive ability), acetylcholinesterase (AChE) activity along with other neurotransmitters content determination, estimation of various oxidative stress parameters and analysis of zinc content in the brain as well as plasma. Histopathological evaluation of the brain hippocampus of each group was performed to corroborate the statistical results. Spherical ZnOTP of 10 to 20 nm size embedded with different phytoconstituents of TP was confirmed. Results of our study revealed a significant memory deficit in mice treated with ZnOTP. Neuronal degeneration was also observed via a significant increase in AChE activity and oxidative stress levels in the brain of mice administered with ZnOTP. Exposure of ZnOTP was also found responsible for modulation of neurotransmission in hippocampus area. Further, ZnOTP disturbed the zinc homeostasis in hippocampus via elevation of zinc content in brain as well as plasma. Histopathology of hippocampus supported the damaging impact of ZnOTP by an increase in vacuolated cytoplasm and focal gliosis in groups treated with ZnOTP. Results demonstrated the neurotoxic effect of ZnOTP on brain hippocampus via cognitive impairment by alteration of neurotransmitter level, zinc content and oxidative stress.

show abstract

Theory and Practice of Histological Techniques

Abstract: Book reviews Evaluation and Management of Hospital Infections. New Perspectives in Clinical Microbiology no 5. Ed Ralph van Furth.

Cited by 30 publications

References 0 publications

Comparative Evaluation of Prosopis cineraria (L.) Druce and Its ZnO Nanoparticles on Scopolamine Induced Amnesia

Comparative Evaluation of Prosopis cineraria (L.) Druce and Its ZnO Nanoparticles on Scopolamine Induced Amnesia

Comparison between Conventional Decalcification and a Microwave-Assisted Method in Bone Tissue Affected with Mycetoma

Biofabricated zinc oxide nanoparticles impair cognitive function via modulating oxidative stress and acetylcholinesterase level in mice

Contact Info

Product

Resources

About