Theory for the sequence of human and rat enamel dissolution by acid and by EDTA: A correlated scanning and transmission electron microscope study

Simmelink, J.W.; Nygaard, V.K.; Scott, David B.

doi:10.1016/0003-9969(74)90213-1

Cited by 51 publications

(31 citation statements)

References 18 publications

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“…3 ± 13.4 nm, respectively (Kerebel et al, 1979); however, the length is in the range of 100-1000 nm (Tesch et al, 2001). Therefore, the central defects on the large crystallite faces provide active dissolution sites, and result in rapid fragmentation of the crystals (Simmelink et al, 1974;Jongebloed et al, 1975) into small pieces (Figs. 4a, 4b).…”

Section: Discussionmentioning

confidence: 99%

Enamel Demineralization in Primary and Permanent Teeth

et al. 2006

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Although enamel demineralization is important for our understanding of caries formation, no consensus has been reached regarding the possible differences in susceptibility of primary and permanent enamel. We used the constant composition (CC) technique to investigate the acid-induced demineralization of these tissues at a relative undersaturation with respect to hydroxyapatite (HAP) of 0.902, pH = 4.5, and ionic strength = 0.15 mol L −1 . The demineralization rates showed significant differences, primary enamel having the greater susceptibility to dissolution during an initial linear stage: 1.5 ± 0.5 × 10 −10 mol mm −2 min −1 compared with 2.6 ± 0.5 × 10 −11 mol mm −2 min −1 for permanent enamel. During the reactions, we observed nanosized crystallites which attached to the enamel surfaces or escaped into the bulk solution. These nanosized crystallites were kinetically protected against further dissolution, even though the solutions remained undersaturated. It is hypothesized that they may contribute to the remarkable mechanical and dynamic characteristics of enamel.

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Section: Discussionmentioning

confidence: 99%

Enamel Demineralization in Primary and Permanent Teeth

et al. 2006

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“…Ultrastructural studies con ducted later were not able to confirm Darl ing 's [1958] hypothesis, but suggested that structural configurations at the prismatic level represented major potential path ways for diffusion [Frank and Brendel, 1966;Johnson, 1967;Mortimer and Tran ter, 1971;Scott et al, 1974;Jongehloed et al, 1975a, b;Haikelel al., 1983;Theuns et al, 1982;Ingram and Fejerskov, 1984], Some workers using the transmission electron microscope also noted that carious destruction may not be as dependent on the structural detail of the tissue as previously believed [Johnson, 1967]; and that carious invasion is, in ad dition to larger pathways, able to follow narrow lanes, such as intercrystalline spaces [Frank and Brendel, 1966]. Ultrastructural studies aimed at the effect of acids on individual enamel crystals have focussed mainly on the role of disloca tions [Daculsi and Kerehel, 1977], with particular emphasis on the preferential central dissolution of individual crystals [Johnson, 1967;Simmelink et al, 1974;Jongehloed et al, 1975a, b;Arends and Jongehloed, 1977;Voegel and Frank, 1977]. The resolving power of our SEM equipment is close to the size of central hole diameters reported in the literature (5-10 nm [Arends, 1982]) which may ex plain why the phenomenon was never re cognized in the surface layer of lesions.…”

Section: Discussionmentioning

confidence: 99%

A Scanning Electron Microscopic Study of Progressive Stages of Enamel Caries in vivo

Holmen

Thylstrup

Øgaard³

et al. 1985

Caries Res

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The present study was carried out to describe surface changes in the scanning electron microscope as related to different periods of carious dissolution in vivo. To ensure accumulation of microbial plaque, orthodontic bands with a standardized buccal space were placed for periods of 1, 2, 3 and 4 weeks on permanent premolars prior to extraction. Examinations in polarized light disclosed a gradual development of a subsurface lesion over time. In the SEM direct dissolution of the surface itself was a prominent feature at all stages of lesion development causing a universal enlargement of intercrystalline spaces. Results indicate that enlarged intercrystalline pathways provide the most important routes for the transport of minerals out of the enamel. The relative protection offered to the outermost crystals at any stage of lesion formation may be an indication of inhibitors acting at the plaque/enamel interface and/or calcium and phosphate present in the plaque.

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“…The maximum depth mea sured by a micrometer was about 20-30 pm or 100 pm, so that ground planes up to 20-30 pm from the enamel surfaces and the middle enamel layers [Matsui et al, 1958] were obtained. The ground planes and the ground sections already observed by polar ized light and the DIC were etched with EDTA which markedly af fected prism boundaries [Hoffman et al 1969: Johnsen et al, 1971Simmelink et al, 1974]. In the present study, the specimens were treated with I % EDTA at pH 7.2 for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Structure of the So-Called ‘Prismless’ Enamel in Human Deciduous Teeth

Kodaka

Nakajima

Higashi³

1989

Caries Res

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The surface enamel of human deciduous teeth showing a more negative birefringence by polarized light was investigated by differential interference contrast microscopy and by scanning electron microscopy (SEM) following EDTA etching. Though this surface enamel has been generally called the ‘prismless’ enamel, in this study, the so-called ‘prismless’ enamel was categorized into ‘false’, ‘moderate’, ‘essential’ and ‘complex’ types according to whether they took the form of distinct prisms which bended at the subsurface, indistinct circularly based prisms or not. Based on the SEM observations some types of the ‘prismless’ enamel showed parallel crystallites and no prism boundaries. However, if indistinct prisms showing centripetal crystallites within the circular boundaries are admitted into the prismless enamel, other types except the ‘false’ one will generally belong to it.

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Theory for the sequence of human and rat enamel dissolution by acid and by EDTA: A correlated scanning and transmission electron microscope study

Cited by 51 publications

References 18 publications

Enamel Demineralization in Primary and Permanent Teeth

Enamel Demineralization in Primary and Permanent Teeth

A Scanning Electron Microscopic Study of Progressive Stages of Enamel Caries in vivo

Structure of the So-Called ‘Prismless’ Enamel in Human Deciduous Teeth

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