Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly

Lamson, Adam; Edelmaier, C. J.; Glaser, Matthew A.; Betterton, Meredith D.

doi:10.1101/419135

Cited by 4 publications

(1 citation statement)

References 103 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In the "unbounded" growth regime, the average MT length increases indefinitely. Forms of the J equation, most commonly J DI , have been utilized in many other papers (e.g., (Gliksman et al, 1992;Bicout, 1997;Maly, 2002;Vorobjev & Maly, 2008;Mirny & Needleman, 2010;Yarahmadian, Barker, Zumbrun, & Shaw, 2011;Mahrooghy, Yarahmadian, Menon, Rezania, & Tuszynski, 2015;Ishihara et al, 2016;Aparna, Padinhateeri, & Das, 2017;Lamson, Edelmaier, Glaser, & Betterton, 2019;Kuo, Trottier, Mahamdeh, & Howard, 2019)).…”

Section: Outline Of Approachmentioning

confidence: 99%

Relationship between Dynamic Instability of Individual Microtubules and Flux of Subunits into and out of Polymer

Mauro

Jonasson

Goodson

2019

Preprint

View full text Add to dashboard Cite

Behaviors of dynamic polymers such as microtubules and actin are frequently assessed at one or both of two scales: (i) net assembly or disassembly of bulk polymer, (ii) growth and shortening of individual filaments. Previous work has derived various forms of an equation to relate the rate of change in bulk polymer mass (i.e., flux of subunits into and out of polymer, often abbreviated as "J") to individual filament behaviors. However, these versions of this "J equation" differ in the variables used to quantify individual filament behavior, which correspond to different experimental approaches. For example, some variants of the J equation use dynamic instability parameters, obtained by following particular individuals for long periods of time. Another form of the equation uses measurements from many individuals followed over short time steps. We use a combination of derivations and computer simulations that mimic experiments to (i) relate the various forms of the J equation to each other; (ii) determine conditions under which these J equation forms are and are not equivalent; and (iii) identify aspects of the measurements that can affect the accuracy of each form of the J equation. Improved understanding of the J equation and its connections to experimentally measurable quantities will contribute to efforts to build a multi-scale understanding of steady-state polymer behavior.

show abstract

Section: Outline Of Approachmentioning

confidence: 99%

Relationship between Dynamic Instability of Individual Microtubules and Flux of Subunits into and out of Polymer

Mauro

Jonasson

Goodson

2019

Preprint

View full text Add to dashboard Cite

show abstract

Distinct regions of the kinesin-5 C-terminal tail are essential for mitotic spindle midzone localization and sliding force

Gergely,

Jones,

Zhou

et al. 2023

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

View full text Add to dashboard Cite

Kinesin-5 motor proteins play essential roles during mitosis in most organisms. Their tetrameric structure and plus-end-directed motility allow them to bind to and move along antiparallel microtubules, thereby pushing spindle poles apart to assemble a bipolar spindle. Recent work has shown that the C-terminal tail is particularly important to kinesin-5 function: The tail affects motor domain structure, ATP hydrolysis, motility, clustering, and sliding force measured for purified motors, as well as motility, clustering, and spindle assembly in cells. Because previous work has focused on presence or absence of the entire tail, the functionally important regions of the tail remain to be identified. We have therefore characterized a series of kinesin-5/Cut7 tail truncation alleles in fission yeast. Partial truncation causes mitotic defects and temperature-sensitive growth, while further truncation that removes the conserved BimC motif is lethal. We compared the sliding force generated by cut7 mutants using a kinesin-14 mutant background in which some microtubules detach from the spindle poles and are pushed into the nuclear envelope. These Cut7-driven protrusions decreased as more of the tail was truncated, and the most severe truncations produced no observable protrusions. Our observations suggest that the C-terminal tail of Cut7p contributes to both sliding force and midzone localization. In the context of sequential tail truncation, the BimC motif and adjacent C-terminal amino acids are particularly important for sliding force. In addition, moderate tail truncation increases midzone localization, but further truncation of residues N-terminal to the BimC motif decreases midzone localization.

show abstract

Distinct regions of the kinesin-5 C-terminal tail are essential for mitotic spindle midzone localization and sliding force

Gergely

Jones

Zhou

et al. 2023

Preprint

View full text Add to dashboard Cite

Kinesin-5 motor proteins play essential roles during mitosis in most organisms. Their tetrameric structure and plus-end-directed motility allow them to bind to and move along antiparallel microtubules, thereby pushing spindle poles apart to assemble a bipolar spindle. Recent work has shown that the C-terminal tail is particularly important to kinesin-5 function: the tail affects motor domain structure, ATP hydrolysis, motility, clustering, and sliding force measured for purified motors, as well as motility, clustering, and spindle assembly in cells. Because previous work has focused on presence or absence of the entire tail, the functionally important regions of the tail remain to be identified. We have therefore characterized a series of kinesin-5/Cut7 tail truncation alleles in fission yeast. Partial truncation causes mitotic defects and temperature-sensitive growth, while further truncation that removes the conserved BimC motif is lethal. We compared the sliding force generated bycuts7mutants using a kinesin-14 mutant background in which some microtubules detach from the spindle poles and are pushed into the nuclear envelope. These Cut7 driven protrusions decreased as more of the tail was truncated, and the most severe truncations produced no observable protrusions. Our observations suggest that the BimC motif and adjacent C-terminal amino acids are essential for sliding force, while the residues N-terminal to the BimC motif are more important for midzone localization. Spindle microtubule protrusions may be a useful tool to study sliding force generated by kinesin-5 motors.

show abstract

Theory of cytoskeletal reorganization during crosslinker-mediated mitotic spindle assembly

Cited by 4 publications

References 103 publications

Relationship between Dynamic Instability of Individual Microtubules and Flux of Subunits into and out of Polymer

Relationship between Dynamic Instability of Individual Microtubules and Flux of Subunits into and out of Polymer

Distinct regions of the kinesin-5 C-terminal tail are essential for mitotic spindle midzone localization and sliding force

Distinct regions of the kinesin-5 C-terminal tail are essential for mitotic spindle midzone localization and sliding force

Contact Info

Product

Resources

About