Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Slater, Gary W.; Guillouzic, Steve; Gauthier, Michael G.; Mercier, Jean François; Kenward, Martin; McCormick, Laurette C.; Tessier, Frédéric

doi:10.1002/elps.200290002

Cited by 76 publications

(82 citation statements)

References 210 publications

(320 reference statements)

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“…Ogston sieving ͑Ogston, 1958; Rodbard and Chrambach, 1970͒ is fundamental for describing the mobility of globular molecules in gel electrophoresis, which is widely used to separate molecules by size ͑Giddings et de Gennes, 1971;Semenov et al, 1995;Viovy, 2000;Slater et al, 2002͒. The sieving mechanism is described by a partitioning process ͑see Sec.…”

Section: B Ogston Sievingmentioning

confidence: 99%

Transport phenomena in nanofluidics

2008

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The transport of fluid in and around nanometer-sized objects with at least one characteristic dimension below 100 nm enables the occurrence of phenomena that are impossible at bigger length scales. This research field was only recently termed nanofluidics, but it has deep roots in science and technology. Nanofluidics has experienced considerable growth in recent years, as is confirmed by significant scientific and practical achievements. This review focuses on the physical properties and operational mechanisms of the most common structures, such as nanometer-sized openings and nanowires in solution on a chip. Since the surface-to-volume ratio increases with miniaturization, this ratio is high in nanochannels, resulting in surface-charge-governed transport, which allows ion separation and is described by a comprehensive electrokinetic theory. The charge selectivity is most pronounced if the Debye screening length is comparable to the smallest dimension of the nanochannel cross section, leading to a predominantly counterion containing nanometer-sized aperture. These unique properties contribute to the charge-based partitioning of biomolecules at the microchannel-nanochannel interface. Additionally, at this free-energy barrier, size-based partitioning can be achieved when biomolecules and nanoconstrictions have similar dimensions. Furthermore, nanopores and nanowires are rooted in interesting physical concepts, and since these structures demonstrate sensitive, label-free, and real-time electrical detection of biomolecules, the technologies hold great promise for the life sciences. The purpose of this review is to describe physical mechanisms on the nanometer scale where new phenomena occur, in order to exploit these unique properties and realize integrated sample preparation and analysis systems.

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Section: B Ogston Sievingmentioning

confidence: 99%

Transport phenomena in nanofluidics

2008

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“…4A demonstrates the length independence of μ for long NAs. Experimental and theoretical studies of polyion electrophoresis phenomena have been reviewed extensively (Allison et al, 2007;Hoagland et al, 1999;Slater et al, 2009;Slater et al, 2002;Viovy, 2000). …”

Section: Electrophoretic Mobility Of Polymeric Nucleic Acidsmentioning

confidence: 99%

Role and Applications of Electrostatic Effects on Nucleic Acid Conformational Transitions and Binding Processes

Ballin¹,

Wilson²

2011

Application of Thermodynamics to Biological and Materials Science

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“…(For recent reviews of theory and simulation of GE, see Refs. [7,11,12].) On the other hand, the observable nonlinear field dependence of the velocity of the model molecule migrating over the lattice mimicking the gel opens a possibility to investigate the nature of Brownian ratchets [3] relevant to GE.…”

Section: Introductionmentioning

confidence: 99%

Diffusion of DNA Molecules in Gel at High Electric Fields

Paściak¹,

Krawczyk²,

Gudowska–Nowak³

et al. 2005

J Biol Phys

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Abstract. Problems related to the gel electrophoretic migration of polymers can be investigated by models based on a Brownian-type ratchet where a particle can undergo a net transport on a potential energy surface that is externally driven to fluctuate between several distinct states. Here we describe the method of polymer transport and separation by means of the cellular automata technique. Numerical simulations of the polymer reptation in the model system allow us to understand the band-broadening processes in the gel electrophoresis experiments. They indicate also possible ways of fine-tuning the parameters in designing desired resolution of the experiments.

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Theory of DNA electrophoresis (∼ 1999 –2002 ½)

Cited by 76 publications

References 210 publications

Transport phenomena in nanofluidics

Transport phenomena in nanofluidics

Role and Applications of Electrostatic Effects on Nucleic Acid Conformational Transitions and Binding Processes

Diffusion of DNA Molecules in Gel at High Electric Fields

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